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Mis18bp1 (NM_172578) Mouse Tagged ORF Clone
CAT＃: MG211410
- TrueORF®
Mis18bp1 (tGFP-tagged) - Mouse expressed sequence C79407 (C79407)
ORF Plasmid: DDK
"NM_172578" in other vectors (4)
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CNY 9,600.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6720403H11; C79407; Knl2; mKIAA1903 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211410 representing NM_172578
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGTAACACCTTTGAAGCATTCAGGAATTCATTTGTCTTCTGGAACATTGCAAAGAAGAAATATGC CTCTAGATGCAGTCTTTATTGACAGCATCCCTTCAGGCACACTCACTCCTTTGAAAGATTTGGTAAAATA TCAGAAGTCCTCTCTAAAAGTTAATGGTCATAAAAAGAATCAGTTATTAGAAATAAGAACTTCTAACAAT AAAGATCTATTTCAATCCACCATGTTATCAGAGGCTACCCTTCCAAACAGCTCTCTTGATATCAGTGTTA TAAAGCCCAGCATGGATAGATTAAGAAATGAAATGATTTATGAATCACCAGGTAAAATCTTTCAAAGAAT GAAAGCAAAAGTACAGCGTGACAAACAAGAACAACTGACAAGGAGCAGTAGCATGTTGGGATCACCACAG GGTGAACACACCAAAGACTTTCCTCCTAACACGGATAAGAAAGCTCAGTTGCAACAAACCTATATCTGTG AGGAAAAACAAACGTCGGTCCAATCCAATGACCCTTCATTGGGAGATCCTCCAATTCTGAACCAAGAACA AAAAAATGTTTCAGCTTCGTGCATTTCAAAGAAGGCATTAACTAGAGCCCAGTTTGGCGGACAAGTTCTT CATTCAAAAGAGAGTCCTGTTAGGATCACTGTGTCCAAAAAGAACACATTTGTTTTGGGAGGCATTGATT GTACTTATGAAAAGTTTGAAAATACTGATGTTAACACCATTAGTTCTCTTTGTGTTCCTATTAAGAATCA TAGCCAATCTATTACTTCTGATAATGATGTGACAACAGAAAGGACTGCAAAAGAGGATATTACAGAACCA AATGAAGAGATGATGTCCAGAAGAACTATTCTTCAAGATCCCATAAAGAATACATCTAAAATTAAACGTT CAAGTCCAAGACCTAATTTAACACTATCTGGCCGGTCTCAAAGAAAATGTACAAAGCTTGAAACTGTTGT AAAAGAAGTAAAAAAATATCAGGCAGTCCACCTACAGGAATGGATGATTAAAGTCATCAATAATAATACT GCTATATGTGTAGAAGGAAAGCTGGTAGATATGACTGATGTTTATTGGCATAGCAATGTAATTATAGAGC GGATTAAACACAATGAACTTAGGACCTTATCAGGCAACATTTATATCTTAAAAGGATTGATAGACTCGGT CTCCATGAAAGAAGCAGGATATCCCTGTTATCTCACAAGAAAATTTATGTTTGGATTTCCCCACAACTGG AAGGAACACATTGATAAATTTCTAGAACAATTAAGGGCTGAAAAAAAGAACAAGACCAGACAGGAAACAG CAAGAGTCCAAGAAAAACAAAAATCAAAAAAAAAAGATGCAGAAGATAAAGAAACTTATGTCCTCCAAAA GGCCAGCATCACGTATGACCTTAATGATAATAGCTTAGAGAGAACTGAAGTACCCACTGATCCCTTGAAC TCACTGGAACAGCCTACCTCCGGCAAAGAAAGAAGACACCCGCTTCTCAGTCAGAAGAGAGCTTATGTTT TAATAACACCACTTAGAAACAAAAAGTTGATAGAGCAAAGATGTATAGACTACAGTCTCTCTATTGAAGG AATATCGGACTTTTTCAAAGCAAAGCATCAAGAAGAAAGTGACTCAGATATACATGGAACTCCAAGTTCT ACCAGTAAGTCTCAAGAGACCTTTGAACATAGAGTGGGATTTGAAGGCAATACCAAGGAGGACTGCAATG AATGTGACATAATCACTGCCAGACATATTCAGATACCTTGCCCGAAAAGTAAACAAATGCTCACCAATGA TTTTATGAAAAAGAACAAGTTGCCCTCAAAACTGCAGAAAACTGAAAATCAAATAGGTGTATCACAGTAT TGCCGGTCCTCATCACATTTGTCAAGTGAAGAGAATGAAGTAGAAATTAAAAGTAGAACCAGAGCAAGAA ATACAAAAGAAAGGCTGAATCGGGAGAGAGAAAACACAAACCACATCACAAAAGATATCCTCCTCATTTC AGAAACTGAAGGTGAGAGAGCTTGTTATATCACACCAAAGAGACCTCGATCTTGTTATATCACACCAAAG AGACCTCGATCTTCTGCTAAAGAGTCACATTACAAATCTGCTGTTAGCAAGGACTTTCTCACTGAAGGAA AGGCATCTGATAGAACTAGCAGACAGCTGTTAGACCATCTACCTGGTTTAACTGATGATGAAGAATGGAG TGAGCAAGAGTTACAGAAGCTACATTGTGCTTTTACATCTCTTCCAAAGCACAAACCTGGCTTTTGGTCA GATGTGGCTATGGCAGTAGGTTCTCGAACTGCTGATGAGTGCCAGAAGAAATACACAGAAGAACCCCAAG GCCAAGGATCCCGAAAACATGGCTCTAAGAAAAAGCAAGCCAACAAGGTCCAAAATGGTGAGAAAGACAG TGCTGATGCCAAGACTATTAAGATAACTGCCAAAGTGGGAACTCTTAAAAGGAAACGACAAATGAGGGAT TGCCTAGAACATTTGGCAAAAGACAACCATGATGATTTTTTCACTGCAACACCCTTGCAGAAACAAAGAA TTCAGTTGCCAAGTTTTCAATACAGTCAAGATGATGATTTTCTGCTAGATATGGACAGGGATCCAGCATC TCCATCATCAATTATTACTTCACCATTGAGAAGCACTACTCCACAATGTCAGCATTTCAGCCCCAGCATG CTAGCCGCTATAGAAAGGAATAATTGTGATAGATATGTTTACCAAATGCAAAAAAATGCTAAAAAATATG GCAAGAGTAATGGTGGCCTTGTCTGGGGCAACATTAGGAAAAAAACAGTTAAAACTGATCTCTCATCTCC ACCACCAACACGGAAAGCCCTGTTTAACAAAGATTTAGGTAAAAACACTGATATTTCAAAGTATTTCATT GATGATACAGAATCAGATGAAGAAGAGAAAGATTATTACTTTTCCAATTCTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211410 representing NM_172578
Red=Cloning site Green=Tags(s) MIVTPLKHSGIHLSSGTLQRRNMPLDAVFIDSIPSGTLTPLKDLVKYQKSSLKVNGHKKNQLLEIRTSNN KDLFQSTMLSEATLPNSSLDISVIKPSMDRLRNEMIYESPGKIFQRMKAKVQRDKQEQLTRSSSMLGSPQ GEHTKDFPPNTDKKAQLQQTYICEEKQTSVQSNDPSLGDPPILNQEQKNVSASCISKKALTRAQFGGQVL HSKESPVRITVSKKNTFVLGGIDCTYEKFENTDVNTISSLCVPIKNHSQSITSDNDVTTERTAKEDITEP NEEMMSRRTILQDPIKNTSKIKRSSPRPNLTLSGRSQRKCTKLETVVKEVKKYQAVHLQEWMIKVINNNT AICVEGKLVDMTDVYWHSNVIIERIKHNELRTLSGNIYILKGLIDSVSMKEAGYPCYLTRKFMFGFPHNW KEHIDKFLEQLRAEKKNKTRQETARVQEKQKSKKKDAEDKETYVLQKASITYDLNDNSLERTEVPTDPLN SLEQPTSGKERRHPLLSQKRAYVLITPLRNKKLIEQRCIDYSLSIEGISDFFKAKHQEESDSDIHGTPSS TSKSQETFEHRVGFEGNTKEDCNECDIITARHIQIPCPKSKQMLTNDFMKKNKLPSKLQKTENQIGVSQY CRSSSHLSSEENEVEIKSRTRARNTKERLNRERENTNHITKDILLISETEGERACYITPKRPRSCYITPK RPRSSAKESHYKSAVSKDFLTEGKASDRTSRQLLDHLPGLTDDEEWSEQELQKLHCAFTSLPKHKPGFWS DVAMAVGSRTADECQKKYTEEPQGQGSRKHGSKKKQANKVQNGEKDSADAKTIKITAKVGTLKRKRQMRD CLEHLAKDNHDDFFTATPLQKQRIQLPSFQYSQDDDFLLDMDRDPASPSSIITSPLRSTTPQCQHFSPSM LAAIERNNCDRYVYQMQKNAKKYGKSNGGLVWGNIRKKTVKTDLSSPPPTRKALFNKDLGKNTDISKYFI DDTESDEEEKDYYFSNSD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172578 |
| ORF Size | 2994 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172578.2, NP_766166.2 |
| RefSeq Size | 4038 bp |
| RefSeq ORF | 2997 bp |
| Locus ID | 217653 |
| UniProt ID | Q80WQ8 |
| Gene Summary | Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204462 | Mis18bp1 (untagged) - Mouse MIS18 binding protein 1 (Mis18bp1), (10ug) |
CNY 7,320.00 |
|
| MR211410 | Mis18bp1 (Myc-DDK-tagged) - Mouse MIS18 binding protein 1 (Mis18bp1) |
CNY 7,312.00 |
|
| MR211410L3 | Lenti ORF clone of Mis18bp1 (Myc-DDK-tagged) - Mouse MIS18 binding protein 1 (Mis18bp1) |
CNY 10,640.00 |
|
| MR211410L4 | Lenti ORF clone of Mis18bp1 (mGFP-tagged) - Mouse MIS18 binding protein 1 (Mis18bp1) |
CNY 10,640.00 |
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