Rbm12 (BC052473) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SWAN, MGC30712, MGC38279, mKIAA0765
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211399 representing BC052473 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGTCATCCGTTTGCAAGGTCTCCCAATTGTGGCGGGGACCATGGACATTCGCCACTTCTTCT CTGGATTAACCATCCCCGATGGGGGCGTGCATATTGTAGGGGGTGAACTGGGTGAGGCTTTCATCGTTTT TGCCACTGATGAAGATGCAAGGCTTGGTATGATGCGCACAGGTGGTACTATTAAAGGGTCAAAAGTAACA CTATTATTGAGTAGTAAAACAGAAATGCAAAATATGATTGAACTGAGTCGTAGGCGTTTTGAAACTGCCA ACTTAGATATTCCGCCAGCAAATGCTAGTAGATCAGGACCACCACCTAGCTCAGGGATGAGCAGCAGGGT GAACTTGCCAGCAATAGTACCCAACTTTAATAATCCTTCGCCAAGTGTAGTAACTGCTACCACTTCTGTT CATGAGAGCAACAAAAACATACAGACATTTTCCACAGCCAGCGTAGGAACCGCTCCTCCAAGCATGGGGA CTTCTTTTGGGAGCCCAACGTTCAGTTCAACCATTCCAAGTACAGCTTCTCCAATGAACACGGTCCCACC GCCACCGATTCCTCCAATCCCAGCGATGCCATCTTTGCCACCGCTGCCATCCATTCCCCCAATACCAGTT CCTCCTCCGGTACCAACATTGCCTCCTGTGCCACCTGTTCCTCCCATTCCCCCAGTCCCTTCGGTGCCAC CTATGACCACACTGCCTCCTATGTCAGGCATGCCACCCTTGAATCCACCACCTGTGGCACCTTTACCTGC TGGAATGAATGGCTCTGGAGCACCTATCGGTCTGAACAATAATATGAATCCTGTGTTTCTGGGTCCATTA AATCCTGTTAACTCTATACAGATGAATTCTCAAAGCAGCGTGAAGTCTCTTCCCATCAACCCTGATGATC TGTATGTGAGTGTGCATGGAATGCCCTTTTCTGCAATGGAAAATGATGTCAGAGAGTTTTTCCATGGGCT CCGAGTCGATGCAGTGCATTTGTTAAAAGATCATGTAGGTCGAAATAATGGGAATGGATTGGTTAAGTTT CTCTCCCCTCAAGATACATTTGAAGCTTTGAAACGGAACAGAATGCTGATGATTCAACGCTATGTGGAAG TCAGTCCTGCCACAGAGAGACAGTGGGTAGCTGCTGGAGGTCATATCACTTTTAAGCAAAGTATGGGGCC TTCTGGACAAGCCCACCCTCCTCCACAGACACTTCCAAGGTCAAAATCGCCCAGTGGGCAGAAAAGGTCA AGATCAAGATCACCACATGAGGCTGGCTTTTGTGTTTACTTAAAAGGGCTACCATTTGAAGCAGAAAACA AACATGTCATTGATTTTTTTAAGAAGTTGGATATTGTGGAAGATAGTATTTATATAGCTTATGGACCCAA TGGGAAAGCAACTGGTGAAGGCTTTGTAGAATTTAGGAATGATGCTGACTATAAGGCTGCTCTCTGTCGT CATAAACAATACATGGGCAATCGCTTTATTCAAGTTCATCCAATAACTAAGAAAGGTATGCTTGAAAAGA TAGATATGATTCGAAAACGACTTCAGAACTTCAGCTATGACCAGAGGGAACTGGTGTTAAATCCAGAGGG GGAAGTCAGTTCTGCCAAAGTCTGTGCCCATATAACAAACATTCCATTCAGCATCACCAAGATGGATGTT CTTCAGTTCCTAGAAGGAATCCCAGTGGATGAGAGTGCTGTGCATGTTCTTGTTGATAACAATGGGCAAG GTCTAGGGCAAGCATTGGTTCAGTTTAAAACTGAAGATGACGCACATAAATCTGAACACTTACACCGTAA AAAGCTGAATGGGAGAGAAGCTTTTGTTCATATAGTTACCCTAGAAGATATGAGAGAGATTGAGAAAAAT CCCCCAGCCCAAGGAAAAAAGGGTCTAAAGATATCTGTTCCAGGTAATCCTGCAGTACCAGTGATACCTA GTGCAGGGATGCCTGCTGCTGGAATTCCCACTGCCGGGATACCAGGGGCTGGATTGCCTAGTGCTGGAAT GCCTGGTGCTGGGATGCCCAGCTCTGGAATGCCTGGTCCTGGAATGCCCGGTCCCGGAATACCCGGTGCG GGAATACCTGGTCCCGCAATGCCTGGTCCTGCAATGCCTGGTCCCGCAATGCCTGGTCCCGCAATGCCCG GTCCCGCAATGCCCGGTCCCGCAATGCCCGGTCCCGCAATGCCCGGTCCCGCAATGCCCGGTCCCGCAAT ACCTGGTCCCGCAATACCTGGTCCCGCAATACCTGGTGCTGGCATCCCCAGTGCAGGAGGAGAAGAGCAT GTCTTCTTGACTGTAGGATCAAAGGAGGCCAATAATGGGCCACCATTTAACTTCCCTGGTAATTTTGGTG GGCCCAATGCCTTTGGGCCACCACTCCCCCCTCCAGGATTAGGGGGTGCCTTTGGTGATGTTAGGCCTGT TATGCCTTCGGTTGGAAATAGTGGTTTGCCTGGCCTAGGACTGGAAGTTCCAGGTTTTGGAGGTGCACCA AATAATATAAGTGGGCCATCAGGATTTGGGGGGATCCCTCAGAACTTTGGAAATGGCCCAGGTAGTTTAA ATGCTCCTCCTGGTTTTGGAAGTGGACCCCCTGGTCTTGGAAGTGTTCCTGGGCATTTGAGTGGGCCTCC AGCCTTTGGGCCTGGGCCTGGGCCTGGCCCAATCCATATTGGGGGTCCCCCTGGCTTTGGAGCTAGCTCT GGAAAACCAGGACCTACAATAATTAAGGTGCAGAACATGCCTTTTACTGTATCTATTGATGAAATTTTAG ATTTCTTTTATGGTTATCAAGTAATTCCAGGTTCAGTATGTTTAAAATACAATGAAAAAGGTATGCCTAC AGGTGAAGCCATGGTGGCCTTTGAATCTCGGGATGAAGCCACAGCTGCTGTCATTGACTTAAATGATAGG CCTATAGGTTCAAGAAAGGTAAAACTTGTTTTAGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211399 representing BC052473 Red=Cloning site Green=Tags(s) MAVVIRLQGLPIVAGTMDIRHFFSGLTIPDGGVHIVGGELGEAFIVFATDEDARLGMMRTGGTIKGSKVT LLLSSKTEMQNMIELSRRRFETANLDIPPANASRSGPPPSSGMSSRVNLPAIVPNFNNPSPSVVTATTSV HESNKNIQTFSTASVGTAPPSMGTSFGSPTFSSTIPSTASPMNTVPPPPIPPIPAMPSLPPLPSIPPIPV PPPVPTLPPVPPVPPIPPVPSVPPMTTLPPMSGMPPLNPPPVAPLPAGMNGSGAPIGLNNNMNPVFLGPL NPVNSIQMNSQSSVKSLPINPDDLYVSVHGMPFSAMENDVREFFHGLRVDAVHLLKDHVGRNNGNGLVKF LSPQDTFEALKRNRMLMIQRYVEVSPATERQWVAAGGHITFKQSMGPSGQAHPPPQTLPRSKSPSGQKRS RSRSPHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPNGKATGEGFVEFRNDADYKAALCR HKQYMGNRFIQVHPITKKGMLEKIDMIRKRLQNFSYDQRELVLNPEGEVSSAKVCAHITNIPFSITKMDV LQFLEGIPVDESAVHVLVDNNGQGLGQALVQFKTEDDAHKSEHLHRKKLNGREAFVHIVTLEDMREIEKN PPAQGKKGLKISVPGNPAVPVIPSAGMPAAGIPTAGIPGAGLPSAGMPGAGMPSSGMPGPGMPGPGIPGA GIPGPAMPGPAMPGPAMPGPAMPGPAMPGPAMPGPAMPGPAMPGPAIPGPAIPGPAIPGAGIPSAGGEEH VFLTVGSKEANNGPPFNFPGNFGGPNAFGPPLPPPGLGGAFGDVRPVMPSVGNSGLPGLGLEVPGFGGAP NNISGPSGFGGIPQNFGNGPGSLNAPPGFGSGPPGLGSVPGHLSGPPAFGPGPGPGPIHIGGPPGFGASS GKPGPTIIKVQNMPFTVSIDEILDFFYGYQVIPGSVCLKYNEKGMPTGEAMVAFESRDEATAAVIDLNDR PIGSRKVKLVLG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC052473
ORF Size	2976 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052473, AAH52473
RefSeq Size	3739 bp
RefSeq ORF	2978 bp
Locus ID	75710
Gene Summary	This gene encodes a protein that contains several RNA-binding motifs, potential transmembrane domains, and proline-rich regions. This gene and the gene for copine I overlap at map location 2 H2. Two alternatively spliced transcript variants have been identified for this gene. Both variants encode the same protein. [provided by RefSeq, Jul 2008]

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