Tmc5 (BC108382) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4932443L08Rik; AU016085
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211339 representing BC108382 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCCTTCCACCAGAATAGCAGCTACATGGAGGATGCTGACTACCCTGGTTACTCAGGGTCTCGGA ACCACACACACAACTATTTGGAAACTCAAGATTATCCGGAATTCCCAGGCTCTCAGAACAATCCAGGCTT CCACCACCCCAGAAGCAACCCGCATTCTTCGGGCTCTAGAACAAACCCAGACTACCCCTACTTCCAAGAA GAACCAGATTATCCTGGATCTCTGAGTAACACGGTCTATCAGGACACCAGGAGTCACCCGTTCTCTGCAC ATTCCAGAACAAGGCCAGACTATGAGTCTTCAATTGAGCCAGACTATAATGACTTCAGGAGTGAATCTTA CCACGCAGGTTTACCCATGGAGCCTGACTACCCTGGCTCTCAGAGCCATCCTGGTTTTGCAGGCGTCGGA AGAAGCAGTATGAACTATACAGGCCCTAGAACCAATCTAGGCTATTTGGGATCTCTGGAAGAACCAGACT ACCCTGGAGCTCAGGATAACTCTTATTATCATTCTGGCTCCAGACCCCATTCCAACCTGCCAGGCTCCAG AAGAGATGCAGGTTATGCTGGGTCCAGAATCAATTCCTACCCAGATGATTTGGGGGAACCTGATTACCCA GGTGCTGAGAATCAACCAAACTCTCCCCGTTTTTACGGAAAGCCAGACTATCCTGGTGCTGAAGAGGGTG ATGGCTATAGCTCTTCCAAGTCCCTGGCAGTCATCAGGAGAGGGAATGGCCCCTTTGGCATGCTCGGCAG GAGGAATGAAGATTACCCTGAAGGCATTGGAATGGTATCCATGGAGATGGCAGGTGATCCTAGAAATGGC TATGTGAATCCTGCTTACACAAGAAGAATTAGTCCTGTCTGTCCAGATAGAAACCTATTGCTGTGCGCAC ATGATTGGCACACATCAGTCCAAGGACAGAAACTAATTGCATCCCTGATTCCCATGACAACTAGAGACAG AATCAAAACCATCAGGAACCAGCCAAGGACCATGCAAGAGAAGAGAGAGCTTAGAAAAATAGTTGACAAA GAAAAAAACAAACAGTCACATGGCATCCTCGAGGCCAACTGCTGTGCTCAGTGTCTGGGCTCCCTTTCAC TGACTTACCGCAGGACCAGGAACGGCCTTTCAGAGCTCCTCAATTACATCACCTTGTGGCAGAAGAGATT CAAGGTCATCGGAGGCAAGTTTGGAACCAGCGTCCTGTCCTATTTCAGCTTCCTGAGGTGGCTTTTGAAG TTCAACATCTTCTCATTCGTCATGAACTTCAGCTTCATCATCATCCCACAATTCACCGTGGGTGCAAAGA ACACCCTCCAGTTCACTGGCTTGGAGTTTTTCACAGGGGCGGGTTATTTTGGGGACACGGTGATGTACTA TGGGTTCTACACCAATTCTACAATCCGACATAGGATGGGTGGAGCATCCTACAACATGCAGCTGGCCTAC ATCTTCACGATCGGAGCCTGCCTGGTCGTCTGTTTCTTCAGTCTACTCTTCAGTATGGCCAAGTACTTCC GGAACAACTTCATCAACCCCCACATATACTCCAGAGGGATTGCTAAGCTCATCTTTTGCTGGGACTTCAC TGTCACCCATGAAAAAGCTGTAAAGCTAAAACAGAAGAATCTGAGCACGGAGATCAGGGAGAACCTGTCT GAGCTCCGCCAGGAGAACTACAGGCTCACATTCAACCAGCAGCTGACCCGATTTTCTGCTCACGTAGCCG CTTGGCTCGTCTCTACTGGAGTGACCGCAGCCTGCTGTGTAGCTGTCTACTACTTGGCTGAGTATAACTC TGAGTTCCTGAAGACTCACAGGAACCCTGGGGCGGTGTTGTTGCTGCCCTTTGTTGTGTCCTGCATCAAC CTGGCCGTGCCTCGCTTCTACTCCATGTTCCGCCTGGTGGAGAGGTATGAGATACCAAGACAGGAAGTCT ACGTCCTCTTGGTCCGAAACATCTTTTTGAAGATCTCCATTGTTGGAATTCTTTGTTACTATTGGCTCAA CATCGTGGCCCTGTCTGGCGAAGAGTGCTGGGAGACCCTCATTGGCCAGGACATCTACCGGCTCCTTCTC ATGGACTTCGTGTTCTCCTTGGCCGATTCCTTACTGGGGGAGTTTCTGAGGAGGCTCATTGGGATGAAGT TCACCAGTCTCAGCCTACAGGAGTTTGACATTGCCAGGAATGTTCTAGAACTGATCTACGCACAAACTCT GACCTGGCTTGGAATTTTCTTCTGCCCTTTGCTGCCTTTTATCCAAATGATCACTCTCTTCATTATGTTC TATGTCAAAAACGTCAGCCTGATGATGAACTTCCAGCCCCCAAGCAAGGCCTGGCGGGCCTCACAGATGA TAACTTTCTTCATTTTCTTGCTCTTCTTCCCATCCTTCACGGGAGTGCTGTGCACCCTGGCTATCACCAT CTGGAGACTGAAACCTTCGGCTGACTGTGGCCCGTTCCGAGGTCTGCCTTCCTTCATCCAATCCATCTAC AGCTGGATTGACACTCTGAGCCGCAGGCCTGGCTACCTGTGGGTCGTCTGGATCTACCAGAATCTCATTG GAAGTGTGCACTTCTTCTTCATCCTCACCCTCATTGTGCTAATCATCACCTATCTTTACTGGCAGATCAC AGAGGGGAGGAAGGTCATGATTCGATTGCTCCATGAACAGATCATTAATGAGGGCAAAGATAAGATGTTC CTGATAGAGAAATTGACCAAACTACAGGATATGGAGAAGAGAGTCAACCCCAGTGCCCTTGACCTGGAAA GGAGAGAGGTGGAGCCACAAATTCCTTTGCATTTGGAGGAACTTGGTGCTGCTCCTGACCTGCGATTGAG GCGGTCAGCACAAGAGGAGAATCCCATAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211339 representing BC108382 Red=Cloning site Green=Tags(s) MSSFHQNSSYMEDADYPGYSGSRNHTHNYLETQDYPEFPGSQNNPGFHHPRSNPHSSGSRTNPDYPYFQE EPDYPGSLSNTVYQDTRSHPFSAHSRTRPDYESSIEPDYNDFRSESYHAGLPMEPDYPGSQSHPGFAGVG RSSMNYTGPRTNLGYLGSLEEPDYPGAQDNSYYHSGSRPHSNLPGSRRDAGYAGSRINSYPDDLGEPDYP GAENQPNSPRFYGKPDYPGAEEGDGYSSSKSLAVIRRGNGPFGMLGRRNEDYPEGIGMVSMEMAGDPRNG YVNPAYTRRISPVCPDRNLLLCAHDWHTSVQGQKLIASLIPMTTRDRIKTIRNQPRTMQEKRELRKIVDK EKNKQSHGILEANCCAQCLGSLSLTYRRTRNGLSELLNYITLWQKRFKVIGGKFGTSVLSYFSFLRWLLK FNIFSFVMNFSFIIIPQFTVGAKNTLQFTGLEFFTGAGYFGDTVMYYGFYTNSTIRHRMGGASYNMQLAY IFTIGACLVVCFFSLLFSMAKYFRNNFINPHIYSRGIAKLIFCWDFTVTHEKAVKLKQKNLSTEIRENLS ELRQENYRLTFNQQLTRFSAHVAAWLVSTGVTAACCVAVYYLAEYNSEFLKTHRNPGAVLLLPFVVSCIN LAVPRFYSMFRLVERYEIPRQEVYVLLVRNIFLKISIVGILCYYWLNIVALSGEECWETLIGQDIYRLLL MDFVFSLADSLLGEFLRRLIGMKFTSLSLQEFDIARNVLELIYAQTLTWLGIFFCPLLPFIQMITLFIMF YVKNVSLMMNFQPPSKAWRASQMITFFIFLLFFPSFTGVLCTLAITIWRLKPSADCGPFRGLPSFIQSIY SWIDTLSRRPGYLWVVWIYQNLIGSVHFFFILTLIVLIITYLYWQITEGRKVMIRLLHEQIINEGKDKMF LIEKLTKLQDMEKRVNPSALDLERREVEPQIPLHLEELGAAPDLRLRRSAQEENPIA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC108382
ORF Size	2901 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC108382, AAI08383
RefSeq Size	3442 bp
RefSeq ORF	2903 bp
Locus ID	74424
Gene Summary	Probable ion channel.[UniProtKB/Swiss-Prot Function]

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