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Sart3 (NM_016926) Mouse Tagged ORF Clone
CAT＃: MG211322
- TrueORF®
Sart3 (tGFP-tagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 3 (Sart3)
ORF Plasmid: DDK
"NM_016926" in other vectors (3)
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CNY 12,168.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AU045857; mKIAA0156 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211322 representing NM_016926
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGACGGCCGCATCTTCGGCGTCCGAGCCGGAGGTTGAGCCCCAGGCCGGGCCTGAGGCCGAGG GAGAAGAGGATGAGGCGAAGCCGGCCGGTGTGCAGCGGAAGGTGCTGTCCGGCGCTGTGGCCGCGGAGGC GGCGGAGGCCAAGGGCCCCGGATGGGACCTGCAGCGGGAAGGCGCGAGCGGGAGCGATGGGGATGAGGAG GACGCCATGGCTTCCTCCGCCGAGAGCTCCGCCGGGGAGGACGAGTGGGAGTACGACGAGGAGGAGGAGA AGAACCAGCTGGAGATCGAGCGGCTGGAGGAGCAGCTGTCCATCAATGGCTATGATTACAACTGCCACGT GGAGCTCATCAGGCTGCTGCGGCTGGAAGGCGAGCTCAGCAGAGTGAGGGCGGCCCGCCAGAAGATGAGT GAGCTCTTCCCCCTGACCGAAGAGCTCTGGCTGGAGTGGCTCCACGATGAGATCAGCATGGCCATGGACG GCCTGGACCGCGAGCACGTGTACGAGCTCTTTGAGAGAGCCGTGAAGGACTACATCTGTCCAAACATTTG GCTAGAGTATGGCCAGTACTCAGTTGGTGGCATTGGTCAGAAAGGTGGCCTTGAGAAGGTTCGCTCTGTC TTTGAAAGAGCCCTGTCCTCTGTTGGCCTGCACATGACGAAAGGCCTGGCCATCTGGGAGGCCTACCGAG AGTTTGAAAGCGCCATCGTGGAGGCTGCTCGGCTGGAGAAAGTGCACAGTCTCTTCCGGCGACAGCTGGC GATCCCACTGTACGAGATGGAGGCCACCTTTGCAGAGTATGAAGAATGGTCAGAGGAGCCCATGCCGGAG TCTGTACTTCAGAGCTATCAGAAAGCGCTGGGGCAGCTAGAGAAGTACAAGCCTTACGAGGAAGCGCTGC TGCAGGCAGAGGCCCCTCGGCTGGCGGAATACCAAGCTTACATCGACTTCGAGATGAAAATCGGGGATCC TGCCCGTATTCAGTTGATCTTTGAGCGTGCCCTGGTGGAGAACTGCCTGGTTCCAGACTTATGGATCCGC TACAGTCAGTACCTAGATCGACAGCTGAAAGTTAAGGACTTGGTTTTATCTGTACACAGCCGTGCTGTGC GGAACTGCCCATGGACAGTTGCCCTGTGGAGTCGGTACCTTCTGGCCATGGAGCGACATGGACTGGACCA TCAAACGATTTCTGCGACCTTCGAGAACGCTCTGAGTGCCGGCTTCATCCAGGCCACTGACTATGTGGAG ATCTGGCAGGTGTACCTCGACTACCTGAGGAGAAGGGTTGACTTCAGACAGGACTCTAGCAAGGAGCTGG AAGAGCTGCGGTCCATGTTCACGCGAGCTCTGGAGTACCTGCAGCAGGAGGTTGAGGAGCGTTTCAGCGA GAGTGGGGATCCAAGCTGCCTGATCATGCAGAGCTGGGCTCGGGTTGAGGCTCGCCTGTGCAATAACATG CAGAAAGCCCGAGAGCTCTGGGACAGCATCATGACCAGAGGGAATGCCAAGTACGCCAACATGTGGCTGG AGTATTACAACCTGGAACGGGCACACGGTGACACACAACACTGTCGGAAGGCTCTGCACCGAGCTGTCCA GTGCACGAGTGACTACCCTGAGCACGTCTGTGAAGTGTTGCTCACCATGGAGAGGACAGAAGGGACCTTA GAAGATTGGGATCTAGCCATTCAGAAAACGGAGACGCGCTTGGCTCGTGTGAATGAGCAGAGAATGAAGG CCGCAGAGAAGGAAGCAGCTCTTGTGCAGCAGGAAGAAGAAAAGGCCGAGCAGCGGAAGAAGGTGCGGGC GGAGAAGAAAGCCCTGAAAAAGAAGAAGAAAACGCGAGGTGCCGACAAGCGCAGGGAGGACGAGGACGAG GAGAACGAGTGGGGCGAAGAGGAGGAAGAGCAGCCTTCCAAACGCAGAAGGACGGAGAACAGTCTGGCCT CTGGAGAGGCTTCGGCTATGAAGGAAGAAACAGAGCTCTCCGGGAAATGCTTAACGATAGATGTTGGTCC TCCTTCCAAGCAGAAAGAGAAGGCAGCCTCCCTTAAGCGGGACATGCCCAAGGTGGCTCACGACAGCAGT AAGGACAGTGTCACCGTGTTTGTCAGCAACCTGCCCTACAGCATAGAAGAGCCCGAGGTGAAGCTCAGGC CGCTCTTTGAGGTCTGTGGGGAGGTGGTCCAGATCAGGCCAATTTTCAGCAACCGCGGGGACTTCCGGGG CTACTGCTATGTGGAGTTTGGAGAGGAGAAGTCAGCCCAGCAGGCCCTGGAGCTGGACAGGAAGATTGTG GAGGGCAGGCCGATGTTTGTGTCCCCCTGTGTGGATAAGAGCAAAAACCCTGATTTTAAGGTGTTCAGAT ACAGTACCACCCTGGAGAAACACAAACTCTTCATCTCTGGCCTGCCCTTTTCCTGCACCAAAGAGGAGCT CGAGGACATTTGTAAGGCCCACGGCACCGTCAAGGACCTCAGGCTGGTCACTAACAGGGCTGGCAAGCCG AAGGGCCTGGCGTATGTGGAGTATGAAAACGAGTCCCAGGCGTCCCAGGCAGTGATGAAGATGGACGGCA TGACCATCAGAGAGAATGTCATCAAGGTGGCAATCAGCAATCCCCCTCAGCGAAAAGTCCCAGAGAAGCC AGAAGTGAGGACAGCACCAGGGGCCCCCATGCTCCCCCGGCAGATGTATGGCGCGCGCGGGAAGGGACGG ACCCAGCTCTCTCTTCTTCCTCGAGCTCTGCAGCGCCAGGGTGCTGCTCCTCAGGCTGAGAACGGCCCAG CTCCGGGGCCCGCGGTCGCCCCGTCTGTGGCCACAGAGGCTCCTAAGATGTCCAATGCTGATTTTGCGAA GTTGCTTCTGAGAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211322 representing NM_016926
Red=Cloning site Green=Tags(s) MATTAASSASEPEVEPQAGPEAEGEEDEAKPAGVQRKVLSGAVAAEAAEAKGPGWDLQREGASGSDGDEE DAMASSAESSAGEDEWEYDEEEEKNQLEIERLEEQLSINGYDYNCHVELIRLLRLEGELSRVRAARQKMS ELFPLTEELWLEWLHDEISMAMDGLDREHVYELFERAVKDYICPNIWLEYGQYSVGGIGQKGGLEKVRSV FERALSSVGLHMTKGLAIWEAYREFESAIVEAARLEKVHSLFRRQLAIPLYEMEATFAEYEEWSEEPMPE SVLQSYQKALGQLEKYKPYEEALLQAEAPRLAEYQAYIDFEMKIGDPARIQLIFERALVENCLVPDLWIR YSQYLDRQLKVKDLVLSVHSRAVRNCPWTVALWSRYLLAMERHGLDHQTISATFENALSAGFIQATDYVE IWQVYLDYLRRRVDFRQDSSKELEELRSMFTRALEYLQQEVEERFSESGDPSCLIMQSWARVEARLCNNM QKARELWDSIMTRGNAKYANMWLEYYNLERAHGDTQHCRKALHRAVQCTSDYPEHVCEVLLTMERTEGTL EDWDLAIQKTETRLARVNEQRMKAAEKEAALVQQEEEKAEQRKKVRAEKKALKKKKKTRGADKRREDEDE ENEWGEEEEEQPSKRRRTENSLASGEASAMKEETELSGKCLTIDVGPPSKQKEKAASLKRDMPKVAHDSS KDSVTVFVSNLPYSIEEPEVKLRPLFEVCGEVVQIRPIFSNRGDFRGYCYVEFGEEKSAQQALELDRKIV EGRPMFVSPCVDKSKNPDFKVFRYSTTLEKHKLFISGLPFSCTKEELEDICKAHGTVKDLRLVTNRAGKP KGLAYVEYENESQASQAVMKMDGMTIRENVIKVAISNPPQRKVPEKPEVRTAPGAPMLPRQMYGARGKGR TQLSLLPRALQRQGAAPQAENGPAPGPAVAPSVATEAPKMSNADFAKLLLRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016926 |
| ORF Size | 2886 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016926.2 |
| RefSeq Size | 3586 bp |
| RefSeq ORF | 2889 bp |
| Locus ID | 53890 |
| UniProt ID | Q9JLI8 |
| Gene Summary | U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation. Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites. May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly. May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (By similarity). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (PubMed:21447833).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211322 | Sart3 (Myc-DDK-tagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 3 (Sart3) |
CNY 10,568.00 |
|
| MR211322L3 | Lenti ORF clone of Sart3 (Myc-DDK-tagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 3 (Sart3) |
CNY 11,500.00 |
|
| MR211322L4 | Lenti ORF clone of Sart3 (mGFP-tagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 3 (Sart3) |
CNY 11,500.00 |
