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Nrp1 (NM_008737) Mouse Tagged ORF Clone
CAT＃: MG211197
- TrueORF®
Nrp1 (tGFP-tagged) - Mouse neuropilin 1 (Nrp1)
ORF Plasmid: DDK
"NM_008737" in other vectors (4)
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CNY 11,736.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C530029I03; NP-1; NPN-1; Npn1; Nrp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211197 representing NM_008737
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGGGGGCTGCCGTTGCTGTGCGCCACGCTCGCCCTTGCCCTCGCCCTGGCGGGCGCTTTCCGCA GCGACAAATGTGGCGGGACCATAAAAATCGAAAACCCAGGGTACCTCACATCTCCCGGTTACCCTCATTC TTACCATCCAAGTGAGAAGTGTGAATGGCTAATCCAAGCTCCGGAACCCTACCAGAGAATCATGATCAAC TTCAACCCACATTTCGATTTGGAGGACAGAGACTGCAAGTATGACTACGTGGAAGTAATCGATGGGGAGA ATGAAGGCGGCCGCCTGTGGGGGAAGTTCTGTGGGAAGATTGCACCTTCTCCTGTGGTGTCTTCAGGGCC CTTTCTCTTCATCAAATTTGTCTCTGACTATGAGACACATGGGGCAGGGTTTTCCATCCGCTATGAAATC TTCAAGAGAGGGCCCGAATGTTCTCAGAACTATACAGCACCTACTGGAGTGATAAAGTCCCCTGGGTTCC CTGAAAAATACCCCAACAGCTTGGAGTGCACCTACATCATCTTTGCACCAAAGATGTCTGAGATAATCCT GGAGTTTGAAAGTTTTGACCTGGAGCAAGACTCGAATCCTCCCGGAGGAATGTTCTGTCGCTATGACCGG CTGGAGATCTGGGATGGATTCCCTGAAGTTGGCCCTCACATTGGGCGTTATTGTGGGCAGAAAACTCCTG GCCGGATCCGCTCCTCTTCAGGCGTTCTATCCATGGTCTTTTACACTGACAGCGCAATAGCAAAAGAAGG TTTCTCAGCCAACTACAGTGTGCTACAGAGCAGCATCTCTGAAGATTTTAAGTGTATGGAGGCTCTGGGC ATGGAATCTGGAGAGATCCATTCTGATCAGATCACTGCATCTTCACAGTATGGTACCAACTGGTCTGTAG AGCGCTCCCGCCTGAACTACCCTGAAAATGGGTGGACTCCAGGAGAAGACTCCTACAAGGAGTGGATCCA GGTGGACTTGGGCCTCCTGCGATTCGTTACTGCTGTAGGGACACAGGGTGCCATTTCCAAGGAAACCAAG AAGAAATATTATGTCAAGACTTACAGAGTAGACATCAGCTCCAACGGAGAGGACTGGATCTCCCTGAAAG AGGGAAATAAAGCCATTATCTTTCAGGGAAACACCAACCCCACAGATGTTGTCTTAGGAGTTTTCTCCAA ACCACTGATAACTCGATTTGTCCGAATCAAACCTGTATCCTGGGAAACTGGTATATCTATGAGATTTGAA GTTTATGGCTGCAAGATAACAGATTATCCTTGCTCTGGAATGTTGGGCATGGTGTCTGGACTTATTTCAG ACTCCCAGATTACAGCATCCAATCAAGCCGACAGGAATTGGATGCCAGAAAACATCCGTCTGGTGACCAG TCGTACCGGCTGGGCACTGCCACCCTCACCCCACCCATACACCAATGAATGGCTCCAAGTGGACCTGGGA GATGAGAAGATAGTAAGAGGTGTCATCATTCAGGGTGGGAAGCACCGAGAAAACAAGGTGTTCATGAGGA AGTTCAAGATCGCCTATAGTAACAATGGCTCTGACTGGAAAACTATCATGGATGACAGCAAGCGCAAGGC TAAGTCGTTCGAAGGCAACAACAACTATGACACACCTGAGCTTCGGACGTTTTCACCTCTCTCCACAAGG TTCATCAGGATCTACCCTGAGAGAGCCACACACAGTGGGCTTGGGCTGAGGATGGAGCTACTGGGCTGTG AAGTGGAAGCACCTACAGCTGGACCAACCACACCCAATGGGAACCCAGTGGATGAGTGTGACGACGACCA GGCCAACTGCCACAGTGGCACAGGTGATGACTTCCAGCTCACAGGAGGCACCACTGTCCTGGCCACAGAG AAGCCAACCATTATAGACAGCACCATCCAATCAGAGTTCCCGACATACGGTTTTAACTGCGAGTTTGGCT GGGGCTCTCACAAGACATTCTGCCACTGGGAGCATGACAGCCATGCACAGCTCAGGTGGAGTGTGCTGAC CAGCAAGACAGGGCCGATTCAGGACCATACAGGAGATGGCAACTTCATCTATTCCCAAGCTGATGAAAAT CAGAAAGGCAAAGTAGCCCGCCTGGTGAGCCCTGTGGTCTATTCCCAGAGCTCTGCCCACTGTATGACCT TCTGGTATCACATGTCCGGCTCTCATGTGGGTACACTGAGGGTCAAACTACGCTACCAGAAGCCAGAGGA ATATGATCAACTGGTCTGGATGGTGGTTGGGCACCAAGGAGACCACTGGAAAGAAGGACGTGTCTTGCTG CACAAATCTCTGAAACTATATCAGGTTATTTTTGAAGGTGAAATCGGAAAAGGAAACCTTGGTGGAATTG CTGTGGATGATATCAGTATTAACAACCATATTTCTCAGGAAGACTGTGCAAAACCAACAGACCTAGATAA AAAGAACACAGAAATTAAAATTGATGAAACAGGGAGCACTCCAGGATATGAAGGAGAAGGGGAAGGTGAC AAGAACATCTCCAGGAAGCCAGGCAATGTGCTTAAGACCCTGGATCCCATCCTGATCACCATCATAGCCA TGAGTGCCCTGGGAGTACTCCTGGGTGCAGTCTGTGGAGTTGTGCTGTACTGTGCCTGTTGGCACAATGG GATGTCAGAAAGGAACCTATCTGCCCTGGAGAACTATAACTTTGAACTTGTGGATGGTGTAAAGTTGAAA AAAGATAAACTGAACCCACAGAGTAATTACTCAGAGGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211197 representing NM_008737
Red=Cloning site Green=Tags(s) MERGLPLLCATLALALALAGAFRSDKCGGTIKIENPGYLTSPGYPHSYHPSEKCEWLIQAPEPYQRIMIN FNPHFDLEDRDCKYDYVEVIDGENEGGRLWGKFCGKIAPSPVVSSGPFLFIKFVSDYETHGAGFSIRYEI FKRGPECSQNYTAPTGVIKSPGFPEKYPNSLECTYIIFAPKMSEIILEFESFDLEQDSNPPGGMFCRYDR LEIWDGFPEVGPHIGRYCGQKTPGRIRSSSGVLSMVFYTDSAIAKEGFSANYSVLQSSISEDFKCMEALG MESGEIHSDQITASSQYGTNWSVERSRLNYPENGWTPGEDSYKEWIQVDLGLLRFVTAVGTQGAISKETK KKYYVKTYRVDISSNGEDWISLKEGNKAIIFQGNTNPTDVVLGVFSKPLITRFVRIKPVSWETGISMRFE VYGCKITDYPCSGMLGMVSGLISDSQITASNQADRNWMPENIRLVTSRTGWALPPSPHPYTNEWLQVDLG DEKIVRGVIIQGGKHRENKVFMRKFKIAYSNNGSDWKTIMDDSKRKAKSFEGNNNYDTPELRTFSPLSTR FIRIYPERATHSGLGLRMELLGCEVEAPTAGPTTPNGNPVDECDDDQANCHSGTGDDFQLTGGTTVLATE KPTIIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDSHAQLRWSVLTSKTGPIQDHTGDGNFIYSQADEN QKGKVARLVSPVVYSQSSAHCMTFWYHMSGSHVGTLRVKLRYQKPEEYDQLVWMVVGHQGDHWKEGRVLL HKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHISQEDCAKPTDLDKKNTEIKIDETGSTPGYEGEGEGD KNISRKPGNVLKTLDPILITIIAMSALGVLLGAVCGVVLYCACWHNGMSERNLSALENYNFELVDGVKLK KDKLNPQSNYSEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008737 |
| ORF Size | 2769 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008737.2, NP_032763.2 |
| RefSeq Size | 5921 bp |
| RefSeq ORF | 2772 bp |
| Locus ID | 18186 |
| UniProt ID | P97333 |
| Gene Summary | Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis (By similarity). Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (PubMed:26503042).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222621 | Nrp1 (untagged) - Mouse neuropilin 1 (Nrp1), (10ug) |
CNY 10,152.00 |
|
| MR211197 | Nrp1 (Myc-DDK-tagged) - Mouse neuropilin 1 (Nrp1) |
CNY 10,136.00 |
|
| MR211197L3 | Lenti ORF clone of Nrp1 (Myc-DDK-tagged) - Mouse neuropilin 1 (Nrp1) |
CNY 12,536.00 |
|
| MR211197L4 | Lenti ORF clone of Nrp1 (mGFP-tagged) - Mouse neuropilin 1 (Nrp1) |
CNY 12,536.00 |
