Hk1 (BC072628) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Hk1-s, mHk1-s
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211174 representing BC072628 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCCGCGCAACTACTGGCATATTACTTCACCGAGCTGAAGGATGACCAAGTCAAAAAGATTGATA AGTATCTGTATGCCATGCGGCTCTCTGATGAAATTCTGATAGATATCCTGACACGCTTCAAGAAAGAGAT GAAGAATGGCCTCTCCCGGGATTATAACCCAACGGCCTCCGTCAAGATGCTGCCAACCTTTGTCCGGTCC ATTCCGGACGGCTCAGAAAAGGGGGATTTCATTGCACTGGATCTCGGCGGGTCTTCCTTTCGAATCCTGC GGGTGCAGGTGAACCACGAGAAGAGTCAGAACGTCAGCATGGAGTCTGAGGTCTACGACACCCCAGAGAA CATCGTGCACGGCAGTGGAAGCCAGCTTTTTGATCACGTCGCTGAATGCCTCGGAGACTTCATGGAGAAA AGGAAGATCAAGGACAAGAAATTACCCGTGGGATTCACGTTTTCCTTCCCGTGCCGACAATCCAAAATAG ACGAGGCCGTACTGATCACGTGGACAAAGCGGTTCAAAGCCAGTGGCGTGGAAGGGGCGGATGTGGTCAA GCTGCTGAATAAAGCCATTAAGAAGCGAGGGGACTATGACGCTAACATTGTAGCTGTGGTGAATGACACA GTGGGGACCATGATGACCTGCGGCTACGATGACCAACAGTGTGAAGTCGGCCTGATCATTGGCACTGGCA CCAATGCTTGCTACATGGAGGAACTGCGACACATCGACCTGGTGGAAGGCGATGAGGGGAGGATGTGTAT TAACACGGAATGGGGAGCCTTTGGGGATGATGGGTCCCTGGAAGACATTCGAACAGAGTTTGACAGAGAG TTAGACCGGGGATCCCTCAACCCTGGGAAACAGCTGTTCGAGAAGATGGTGAGCGGCATGTACATGGGGG AGCTGGTCCGGCTGATCCTGGTGAAGATGGCCAAGGAAAGCCTCTTATTTGAAGGGCGCATTACTCCAGA GCTGCTCACGAGGGGCAAGTTCACCACTAGCGACGTAGCCGCCATTGAAACGGATAAGGAAGGCGTTCAA AATGCCAAGGAAATCTTGACCCGCCTGGGAGTGGAGCCGTCTCACGATGACTGCGTATCGGTCCAGCACG TATGCACGATCGTCTCCTTCCGATCAGCCAACCTGGTGGCTGCCACGCTCGGTGCCATCTTGAACCGCCT GCGGGACAATAAGGGCACGCCCAGGCTGCGGACCACAGTTGGCGTAGACGGTTCTCTCTACAAGATGCAC CCACAGTATTCCCGGCGGTTCCACAAGACCCTGAGGCGCCTGGTGCCTGACTCGGACGTCCGGTTCCTCC TCTCGGAGAGTGGCAGTGGCAAGGGAGCCGCCATGGTGACCGCTGTGGCCTACCGCCTGGCCGAGCAGCA CCGGCAGATTGAGGAAACCCTGTCCCACTTCCGCCTCAGCAAGCAGGCACTGATGGAGGTGAAGAAGAAG CTGCGGTCAGAGATGGAAATGGGGCTGAGAAAGGAGACCAACAGCAGAGCTACGGTCAAAATGCTGCCTT CTTATGTTCGGAGCATCCCAGATGGGACTGAGCATGGTGACTTCCTGGCCTTGGATCTCGGAGGAACGAA TTTCCGAGTCCTACTGGTAAAGATCCGTAGTGGGAAAAAGAGAACAGTGGAGATGCACAACAAGATCTAC TCCATTCCCCTGGAAATCATGCAGGGCACCGGGGATGAGCTGTTTGATCACATTGTCTCCTGCATCTCCG ACTTCCTGGACTACATGGGGATCAAAGGCCCCCGGATGCCTCTGGGCTTCACCTTCTCGTTTCCCTGCAA GCAGACGAGCCTAGATTGCGGAATCTTGATCACGTGGACAAAGGGATTCAAAGCCACCGACTGTGTGGGT CACGATGTAGCCACTTTACTGAGGGATGCTGTAAAAAGGAGAGAGGAATTTGACCTGGATGTGGTGGCTG TGGTCAACGACACCGTGGGCACCATGATGACTTGTGCTTATGAAGAACCTTCTTGTGAGATTGGACTCAT CGTGGGGACTGGCAGCAATGCCTGCTACATGGAGGAGATGAAAAACGTGGAGATGGTGGAGGGGAACCAG GGCCAGATGTGCATCAATATGGAATGGGGTGCCTTTGGTGACAACGGCTGTCTGGACGACATCAGAACAG ACTTCGACAAAGTGGTGGACGAATATTCTCTAAACAGTGGGAAACAAAGGTTTGAGAAGATGATCAGTGG AATGTACCTGGGTGAGATCGTCCGTAACATCCTGATTGACTTCACCAAGAAAGGCTTCCTCTTCCGGGGA CAGATCTCTGAGCCACTCAAGACCCGAGGCATCTTCGAGACCAAGTTTCTCTCTCAGATCGAGAGTGACC GATTAGCGCTGCTCCAGGTGCGGGCCATCCTTCAGCAGCTGGGTCTAAACAGCACGTGCGACGACAGTAT CCTGGTCAAGACCGTGTGTGGGGTGGTGTCCAAGCGGGCGGCCCAGCTGTGTGGTGCCGGCATGGCCGCC GTGGTGGAAAAGATCCGAGAGAACAGAGGCCTAGACCACCTGAATGTAACCGTGGGCGTGGACGGGACGC TCTACAAACTCCATCCACACTTCTCCAGAATCATGCACCAAACAGTGAAGGAACTGTCACCAAAGTGTAC CGTGTCCTTCCTCCTGTCTGAAGACGGCAGCGGCAAGGGGGCCGCCCTTATCACAGCTGTGGGCGTGCGG CTCAGAGGAGACCCTACGAACGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211174 representing BC072628 Red=Cloning site Green=Tags(s) MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDEILIDILTRFKKEMKNGLSRDYNPTASVKMLPTFVRS IPDGSEKGDFIALDLGGSSFRILRVQVNHEKSQNVSMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEK RKIKDKKLPVGFTFSFPCRQSKIDEAVLITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDT VGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDRE LDRGSLNPGKQLFEKMVSGMYMGELVRLILVKMAKESLLFEGRITPELLTRGKFTTSDVAAIETDKEGVQ NAKEILTRLGVEPSHDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKMH PQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLSHFRLSKQALMEVKKK LRSEMEMGLRKETNSRATVKMLPSYVRSIPDGTEHGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIY SIPLEIMQGTGDELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCKQTSLDCGILITWTKGFKATDCVG HDVATLLRDAVKRREEFDLDVVAVVNDTVGTMMTCAYEEPSCEIGLIVGTGSNACYMEEMKNVEMVEGNQ GQMCINMEWGAFGDNGCLDDIRTDFDKVVDEYSLNSGKQRFEKMISGMYLGEIVRNILIDFTKKGFLFRG QISEPLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAA VVEKIRENRGLDHLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCTVSFLLSEDGSGKGAALITAVGVR LRGDPTNA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC072628
ORF Size	2756 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC072628, AAH72628
RefSeq Size	4045 bp
RefSeq ORF	2756 bp
Locus ID	15275
Gene Summary	Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).[UniProtKB/Swiss-Prot Function]

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