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Tmem16b (BC033409) Mouse Tagged ORF Clone
CAT＃: MG211157
- TrueORF®
Tmem16b (tGFP-tagged) - Mouse transmembrane protein 16B (cDNA clone MGC:38715 IMAGE:5357763)
ORF Plasmid: DDK
"BC033409" in other vectors (3)
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CNY 10,070.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC38715 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211157 representing BC033409
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTTTCACGACAACCAGAGGAAAGTCGACTATGTACTTGCATACCACTACCGGAAACGAGGCGCAC ACCTGGGTCACGGCTCCCCTGGACACTCTCTGGCTGTTATCTCCAATGGGGAGACAGGCAAGGAGCGCCA TGGCGGGGGCCCTGGTGATGTTGAACTTGGGCCACTGGATGCTCTGGAAGAGGAAAGGAGGGAGCAGCGA GATGAATTTGAGCACAACCTGATGGCTGCTGGGCTGGAGCTGGAGAAGGACTTGGAGAGCAAAAGCCAGG GATCTGTCTTTGTCCGGATCCACGCCCCATGGCAGGTGCTGGCCAGGGAAGCAGAGTTCTTGAAGATCAA AGTCCCCACCAAGAAAATGTACGAGATCAAAGCAGGAGGCAGCATCGCAAAGAAGTTCAGCGCCATTCTG CAGACGCTCAGTTCACCCCTGCAGCCCAGAGTTCCTGAACACAGCAACAACAGGATGAAAAACCTCTCCT ACCCATTCTCCAGGGAAAAAATGTACCTGTATAACATCCAGGAAAAGGACACCTTCTTTGACAATGCTAC CCGGAGCCGCATTGTACACGAGATTCTGAAGCGGACGGCCTGTTCTCGAGCCAACAACACGATGGGTATT AACTCTCTAATAGCAAACAATATCTACGAGGCTGCCTACCCGCTTCATGATGGTGAATATGACAGTCCAG GGGATGACATGAATGACAGAAAGCTGCTGTATCAGGAATGGGCACGCTATGGAGTGTTTTACAAATTTCA ACCCATTGACCTCATCAGGAAGTATTTTGGAGAGAAAATTGGGCTATATTTTGCTTGGCTGGGGTTGTAT ACATCATTCCTGATCCCATCTTCTGTCATAGGCGTGATTGTGTTTCTTTATGGATGTGCAACAATCGAGG AAGATATTCCCAGCAAAGAGATGTGTGACCACCAGAATGCCTTCACCATGTGTCCCCTGTGTGACAAGTC CTGTGACTATTGGAACCTCAGCTCAGCCTGTGGGACTGCACGGGCCAGTCATCTGTTTGACAATCCAGCC ACCGTCTTCTTTTCCATCTTCATGGCTCTGTGGGCTACCATGTTTCTGGAAAACTGGAAGAGACTCCAGA TGCGGTTGGGCTACTTCTGGGACCTGACTGGGATCGAAGAGGAAGAAGAACGTTCCCAGGAACACTCTCG GCCTGAATATGAAACCAAAGTACGAGAGAAACTGTTAAAGGAGAGTGGCAAGTCAGCGGTTCAGAAGCTG GAAGCAAATTCACCTGAGGATGATGAGGATGATGAAGATAAACTGACTTGGAAAGATCGTTTCCCAGGAT ACTTGATGAACTTTGCCTCCATCTTGTTTATGATTGCCCTGACGTTCTCCATTGTCTTTGGGGTCATTGT GTATCGGATCACAACTGCAGCTGCCCTGTCTCTCAACAAGGCCACACGCTCCAATGTCCGGGTGACAGTG ACTGCAACAGCAGTCATCATCAACCTCGTGGTCATCCTCATCTTGGATGAGATCTATGGTGCTGTGGCCA AGTGGCTTACCAAAATCGAGGTTCCGAAAACAGAACAGACTTTTGAAGAACGGTTGATCCTCAAAGCTTT CTTGCTCAAGTTTGTCAATGCCTACTCCCCCATCTTCTATGTGGCCTTTTTCAAGGGGAGGTTTGTGGGC AGACCTGGAAGCTATGTCTACGTGTTCGACGGTTACCGCATGGAAGAGTGTGCTCCGGGAGGCTGTCTCA TGGAGCTGTGCATCCAACTCAGCATCATCATGTTGGGCAAGCAGCTGATCCAGAACAATATCTTTGAGAT TGGAGTCCCGAAGCTAAAGAAACTCTTTCGGAAGCTGAAAGATGAGACAGAGCCTGGAGAAAGTGACCCT GACCACTCAAAACGTCCAGAGCAGTGGGACCTGGACCACAGCCTAGAGCCTTACACTGGGCTGACTCCTG AGTACATGGAAATGATCATCCAGTTTGGTTTTGTCACCCTTTTTGTGGCCTCCTTTCCTCTGGCCCCTGT GTTTGCTCTCCTCAACAATGTCATTGAAGTGCGGCTGGATGCAAAAAAGTTTGTTACAGAACTGAGACGC CCAGATGCTGTGAGGACCAAAGACATTGGAATTTGGTTTGACATTCTCTCTGGAATTGGCAAGTTCTCCG TCATCATTAATGCTTTTGTCATTGCTGTCACCTCCGACTTTATCCCCCGCCTTGTGTACCAGTACTCCTA TAGCCACAACGGGACACTACATGGCTTTGTGAACCATACACTCTCCTTTTTCAACGTCAGTCAGCTGAAG GAGGGAACACAGCCTGAAAACTCACAGTTCGACCAGGAGGTTCAGTTTTGCAGGTTCAAAGATTATCGAG AACCACCATGGGCACCAAACCCTTATGAGTTTTCCAAGCAGTACTGGTCTGTTTTGTCTGCCCGTCTGGC CTTTGTTATCATCTTCCAGAACCTAGTGATGTTCCTGAGTGTCCTGGTGGACTGGATGATTCCGGATATC CCCACTGACATCAGCGACCAGATCAAGAAAGAGAAGAGCTTGCTGGTAGACTTCTTTCTGAAGGAGGAGC ATGAGAAGGTCAAGCTGGCTGATGAGCCCACCCAGAGGAGCCAGGGAGGTGGGGATCGGAGCAGGAGGAG CCGTGCAGCCAGCTCTGCACCATCAGGCCGGAGCCAGCCAGGCAGCATAGCATCCTCAGGGTCCCAGCAC ACCAATGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211157 representing BC033409
Red=Cloning site Green=Tags(s) MHFHDNQRKVDYVLAYHYRKRGAHLGHGSPGHSLAVISNGETGKERHGGGPGDVELGPLDALEEERREQR DEFEHNLMAAGLELEKDLESKSQGSVFVRIHAPWQVLAREAEFLKIKVPTKKMYEIKAGGSIAKKFSAIL QTLSSPLQPRVPEHSNNRMKNLSYPFSREKMYLYNIQEKDTFFDNATRSRIVHEILKRTACSRANNTMGI NSLIANNIYEAAYPLHDGEYDSPGDDMNDRKLLYQEWARYGVFYKFQPIDLIRKYFGEKIGLYFAWLGLY TSFLIPSSVIGVIVFLYGCATIEEDIPSKEMCDHQNAFTMCPLCDKSCDYWNLSSACGTARASHLFDNPA TVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEERSQEHSRPEYETKVREKLLKESGKSAVQKL EANSPEDDEDDEDKLTWKDRFPGYLMNFASILFMIALTFSIVFGVIVYRITTAAALSLNKATRSNVRVTV TATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEERLILKAFLLKFVNAYSPIFYVAFFKGRFVG RPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGKQLIQNNIFEIGVPKLKKLFRKLKDETEPGESDP DHSKRPEQWDLDHSLEPYTGLTPEYMEMIIQFGFVTLFVASFPLAPVFALLNNVIEVRLDAKKFVTELRR PDAVRTKDIGIWFDILSGIGKFSVIINAFVIAVTSDFIPRLVYQYSYSHNGTLHGFVNHTLSFFNVSQLK EGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWSVLSARLAFVIIFQNLVMFLSVLVDWMIPDI PTDISDQIKKEKSLLVDFFLKEEHEKVKLADEPTQRSQGGGDRSRRSRAASSAPSGRSQPGSIASSGSQH TNV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC033409 |
| ORF Size | 2741 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC033409, AAH33409 |
| RefSeq Size | 3544 bp |
| RefSeq ORF | 2741 bp |
| Locus ID | 243634 |
| Gene Summary | Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211157 | Tmem16b (Myc-DDK-tagged) - Mouse transmembrane protein 16B (cDNA clone MGC:38715 IMAGE:5357763) |
CNY 9,120.00 |
|
| MR211157L3 | Lenti ORF clone of Tmem16b (Myc-DDK-tagged) - Mouse transmembrane protein 16B (cDNA clone MGC:38715 IMAGE:5357763) |
CNY 11,020.00 |
|
| MR211157L4 | Lenti ORF clone of Tmem16b (mGFP-tagged) - Mouse transmembrane protein 16B (cDNA clone MGC:38715 IMAGE:5357763) |
CNY 11,020.00 |
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