Aldh1l1 (BC028817) Mouse Tagged ORF Clone

CAT#: MG211113

  • TrueORF®

Aldh1l1 (tGFP-tagged) - Mouse aldehyde dehydrogenase 1 family, member L1 (cDNA clone MGC:36396 IMAGE:5101815)

ORF Plasmid: DDK tGFP



  "BC028817" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,610.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1810048F20Rik; FDH; Fthfd; Neut2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211113 representing BC028817
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATTGCAGTAATCGGGCAGAGCCTGTTTGGCCAGGAGGTTTACTGCCAGCTAAGGAAGGAGGGCC
ATGAGGTGGTGGGTGTGTTCACCATCCCAGACAAGGATGGGAAAGCAGATCCCCTGGGCCTGGAGGCCGA
GAAGGATGGCGTGCCCGTGTTTAAGTTCCCTCGGTGGCGGGCTCGAGGACAGGCTCTGCCCGAGGTCGTG
GCCAAGTACCAGGCTCTGGGTGCAGAACTCAACGTGCTCCCCTTCTGCAGCCAGTTCATACCCATGGAGG
TCATCAATGCCCCCCGGCATGGCTCCATCATCTACCATCCATCTCTGCTGCCCAGGCACCGAGGGGCCTC
AGCCATCAACTGGACCCTCATTCATGGAGATAAGAAAGGAGGCTTCACTATCTTCTGGGCTGATGACGGT
CTGGACACTGGTGACCTTCTGCTGCAGAAGGAGTGTGACGTGCTTCCAGATGACACCGTGAGCACGCTGT
ACAACCGTTTCCTCTTCCCAGAGGGCATCAAAGGGATGGTTCAGGCTGTGCGACTGATTGCAGAGGGCAC
AGCCCCACGCCGTCCCCAGCCTGAGGAAGGAGCCACCTATGAGGGCATTCAGAAAAAGGAGACAGCCATG
ATCAACTGGGACCAGCCAGCAGAGGCCATTCACAACTGGATCCGTGGGAATGACAAGGTGCCAGGTGCCT
GGACAGAGGCTTGTGGGCAGAAGCTGACGTTTTTCAACTCGACACTCAACACTTCAGGACTGGTGGCTCA
GGGAGAAGCTCTACCCATCCCAGGAGCCCATCGTCCAGGCCTAGTCACCAAAGCGGGACTCATCCTCTTT
GGGAATGATGATAGAATGTTGCTGGTGAAGAATATCCAGCTGGAAGATGGCAAGATGATGCCAGCCTCCC
AGTTCTTCAAGGGGTCTGCTAGCAGTGCCCTGGAGCTGACCGAGGAGGAGCTGGCCACAGCAGAGGCCGT
GCGGAGCTCTTGGATGCGAATTCTGCCCAATGTCCCAGAGGTAGAAGACTCTACAGATTTCTTCAAGTCA
GGAGCTGCATCCGTAGATGTTGTGAGGCTGGTGGAGGAGGTGAAGGAGCTGTGTGACGGGCTGGAGTTAG
AAAATGAGGATGTTTACATGGCCACCACCTTCGGGGACTTCATCCAGCTCCTAGTGAGGAAGTTGAGAGG
GGAGGACGGCGAGAGCGAGTGTGTCATTAACTACGTGGAAAAGGCAGTGAAGAAACTGACTCTCCAAATG
CCCTACCAGCTCTTCATAGGCGGCGAGTTTGTGGATGCTGAGGGCGCGAAGACCTACAGTACCATAAACC
CAACGGATGGAAGTGTCATCTGCCAGGTGTCTCTAGCTCAGGTGAGTGATGTTGACAAGGCGGTGGCAGC
AGCGAAGGAGGCCTTTGAGAATGGACTGTGGGGAAAGATAAATGCGCGTGACCGGGGCCGGCTCCTGTAC
AGGTTGGCGGACCTCATGGAGCAGCACCAGGAAGAGCTAGCCACCATTGAGGCCCTGGACGCAGGTGCCG
TCTACACGCTGGCCCTGAAGACGCATGTGGGCATGTCCATCCAGACCTTCCGATACTTCGCTGGCTGGTG
TGATAAGATCCAGGGTGCCACCATCCCCATCAACCAGGCTAGACCCAACCGCAACCTGACCTTGACCAAG
AAGGAACCTGTTGGGGTCTGTGGTATTGTCATCCCCTGGAACTATCCCTTAATGATGCTGTCCTGGAAGA
CAGCAGCCTGCCTGGCTGCCGGGAACACCGTGGTGATCAAGCCTGCCCAGGTGACCCCACTCACAGCCTT
GAAGTTTGCAGAGCTGACACTGAAGGCTGGCATTCCCAAGGGTGTGGTCAACATCCTCCCAGGATCTGGC
TCGCTGGTTGGCCAGAGACTCTCAGACCACCCTGATGTGAGGAAAATAGGGTTCACAGGCTCCACGGAGG
TGGGAAAACACATCATGAAAAGCTGTGCCCTGAGTAATGTGAAGAAGGTCTCCCTGGAGCTGGGTGGAAA
GTCACCCCTTATCATCTTTGCTGACTGTGACCTCAACAAAGCTGTGCAGATGGGCATGAGCTCCGTTTTC
TTCAACAAAGGGGAGAACTGCATTGCGGCAGGCCGGCTCTTTGTGGAGGACTCCATCCATGACCAGTTTG
TGCAGAAAGTGGTGGAGGAAGTAGGGAAGATGAAAATCGGCAACCCCCTGGACAGAGATACCAACCATGG
CCCGCAGAACCATGAGGCCCACCTGAGGAAGCTGGTGGAGTATTGCCAACGTGGTGTGAAGGAAGGGGCC
ACACTGGTCTGTGGTGGGAACCAAGTCCCAAGGCCAGGCTTCTTCTTTCAGCCAACCGTTTTCACAGACG
TGGAGGACCACATGTACATCGCTAAGGAGGAGTCCTTCGGGCCCATCATGATCATCTCTCGGTTTGCTGA
TGGGGACGTGGATGCAGTGCTATCTCGGGCCAATGCTACAGAATTTGGCCTGGCCTCTGGTGTCTTCACT
CGGGATATCAACAAGGCCCTGTATGTCAGTGACAAACTGCAGGCGGGCACTGTGTTTGTCAACACATACA
ACAAGACCGATGTGGCCGCACCTTTTGGAGGATTCAAGCAGTCTGGATTTGGCAAAGACCTGGGAGAGGC
GGCCCTGAATGAGTACCTGCGGATCAAGACTGTGACTTTTGAGTAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211113 representing BC028817
Red=Cloning site Green=Tags(s)

MKIAVIGQSLFGQEVYCQLRKEGHEVVGVFTIPDKDGKADPLGLEAEKDGVPVFKFPRWRARGQALPEVV
AKYQALGAELNVLPFCSQFIPMEVINAPRHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFTIFWADDG
LDTGDLLLQKECDVLPDDTVSTLYNRFLFPEGIKGMVQAVRLIAEGTAPRRPQPEEGATYEGIQKKETAM
INWDQPAEAIHNWIRGNDKVPGAWTEACGQKLTFFNSTLNTSGLVAQGEALPIPGAHRPGLVTKAGLILF
GNDDRMLLVKNIQLEDGKMMPASQFFKGSASSALELTEEELATAEAVRSSWMRILPNVPEVEDSTDFFKS
GAASVDVVRLVEEVKELCDGLELENEDVYMATTFGDFIQLLVRKLRGEDGESECVINYVEKAVKKLTLQM
PYQLFIGGEFVDAEGAKTYSTINPTDGSVICQVSLAQVSDVDKAVAAAKEAFENGLWGKINARDRGRLLY
RLADLMEQHQEELATIEALDAGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGATIPINQARPNRNLTLTK
KEPVGVCGIVIPWNYPLMMLSWKTAACLAAGNTVVIKPAQVTPLTALKFAELTLKAGIPKGVVNILPGSG
SLVGQRLSDHPDVRKIGFTGSTEVGKHIMKSCALSNVKKVSLELGGKSPLIIFADCDLNKAVQMGMSSVF
FNKGENCIAAGRLFVEDSIHDQFVQKVVEEVGKMKIGNPLDRDTNHGPQNHEAHLRKLVEYCQRGVKEGA
TLVCGGNQVPRPGFFFQPTVFTDVEDHMYIAKEESFGPIMIISRFADGDVDAVLSRANATEFGLASGVFT
RDINKALYVSDKLQAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRIKTVTFEY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC028817
ORF Size 2708 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC028817, AAH28817
RefSeq Size 3045 bp
RefSeq ORF 2708 bp
Locus ID 107747
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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