Ect2 (BC032155) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI528536; mKIAA4037
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211053 representing BC032155 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACGACAGCGTGTTACCATCTCCTACTGAGATAACTAGCTTGGCAGACTCTTCAGTGTTTGATT CTAAAGTTGCTGGAATGTCTAAGGAAAACTTGTGCTTGGCGTCTACTTCAAATGTTGATGAGGAAATGCC GCAGGTTGAAGCAAGAGTGATAATGGTCCAGGATGCTGGGAAACAAGAAGAACTTCTAAAGGCCTTAAAG GAAATGAAAGTGCCCTGTGTAAAGATGGACTCAATGGAAGAATTTGAAAGTTTGGATTCCCCAGAATTTG AGAATATATTTGTAGTTACTGACTTCCAGAATTCTGTCTTCAATGACTTATACAAGGCTGATTGTAGGAT TGTTGGGCCACCAGTTATACTCAACTGTGCACAAAGGGGAGAGCCCTTGCCATTTTCCTGCCGGCCGCTG TATTGTACGAGTATGCTGAACCTGGTGCTCTGCTTCACTGGATTCAGGAAGAAGGAGGAGCTTGTCAAAT TGGTGACATTGGTTCATCATATGGGTGGAGTTATTCGAAAAGAGTGTAATTCCAAAGTAACACATCTGGT GGCAAATTGTACACAAGGCGAAAAATTTAGGGTTGCTGTGAGCCTGGGCACTCCAATTATGAAGCCAGAA TGGATTTATAAAGCGTGGGAAAGACGCAATGAACAGTGTTTCTGTGCAGCAGTTGATGACTTTAGAAATG AATTTAAAGTTCCTCCATTCCAAGATTGCATTTTAAGTTTCCTGGGATTTTCAGATGAAGAGAAACATAG TATGGAAGAAATGACTGAAATGCAAGGAGGTAGCTATTTACCAGTTGGGGATGAAAGGTGCACTCACCTT ATTGTTGAAGAGAATACAGTAAAGGACCTTCCATTCGAACCTTCAAAGAAACTTTTTGTTGTCAAGCAAG AGTGGTTCTGGGGAAGCATTCAGATGGACGCTCGTGCAGGAGAGACTATGTATTTGTATGAAAAGGCTAA TACTCCTGAGCTCAAGAAATCGGTGTCTCTGCTTTCTCTAAGTACTCCAAACAGCAACCGCAAGAGACGG AGATTGAAAGAGACCCTGGCTCAGCTCTCCAGGGAGACTGACCTCTCTCCTTTCCCTCCCCGGAAGCGGC CCTCAGCTGAGCATTCTCTTTCTATTGGCTCACTCCTGGATATCTCCAACACACCCGAGTCCAGCATCCA CTATGGAGAAACACCGAAGTCCTGTGCTAAGTCTTCTAGAAGTTCTACTCCAGTTCCTCCAAAGCAGTCA GCCAGGTGGCAGGTGGCGAAAGAGCTCTACCAGACTGAGAGCAATTATGTCAATATACTGGCCACAATCA TTCAATTATTTCAGGTGCCCTTAGAAGAAGAAGGACAGCGTGGTGGGCCTATTCTTGCACCTGAGGAGAT CAAGACTATTTTTGGGAGCATCCCAGATATCTTTGATGTGCATATGAAGATCAAGGATGATCTTGAAGAC CTTATTGCTAACTGGGATGAGAGCAGAAGTATTGGTGACATCTTTCTTAAATATGCAAAAGATTTGGTAA AAACCTACCCTCCGTTTGTAAACTTCTTTGAAATGAGCAAGGAAATGATCATTAAATGTGAGAAACAAAA GCCCAGATTTCATGCTTTCCTCAAGATAAATCAAGCTAAACCAGAATGTGGACGACAGAGCCTTGTGGAA CTTCTCATCCGGCCAGTGCAGAGGCTACCCAGTGTCGCTTTACTTTTAAACGATCTTAAAAAGCATACAG CTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCACTAAAAGAAGTAATGACACATAT CAATGAGGATAAGAGAAAAACAGAAGCACAGAAGCAAATTTTTGATGTTGTTTATGAAGTAGATGGATGC CCAGCTAATCTCTTATCTTCTCATCGGAGCCTGGTGCAACGAGTGGAAACGGTTTCCCTTGGTGAGCACC CTTGCGACCGAGGAGAACAAGTCACTCTCTTCCTCTTCAATGACTGCCTCGAGATAGCAAGAAAGCGGCA CAAGGTTATTGGCACTTTTAGAAGTCCTCACGACCGCACCCGGCCCCCCGCTTCTCTGAAGCACATTCAT CTCATGCCTCTTTCTCAGATTAAGAAGGTGCTGGACATCCGAGAGACAGAAGATTGTCACAATGCCTTTG CCTTGCTTGTAAGGCCACCAACAGAACAGGCCAATGTACTGCTCAGCTTCCAGATGACGTCAGAGGAGCT TCCAAAGGAGAGCTGGCTGAAGATGCTGTGCCGACATGTAGCCAACACCATTTGTAAGGCAGATGCTGAG AATCTTATGTATGTGGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGATAGTACATTGAGTC GAGCATCTAGAGCAATAAAGAAGACTTCAAAAAAGGTTACAAGGGCATTCTCTTTTTCCAAAACTCCCAA GAGAGCCCTGCGGATGGCTCTTTCATCATCCCATAGCTCAGAGGGAAGGAGTCCTCCGAGCAGTGGCAAG GTTGCTGTGAGCCGTCTGTCCAGCACATCATCCTTAGCAGGTATTCCATCTCCCTCCCTTGTCAGCCTCC CTTCTTTCTTTGAACGGAGAAGTCATACATTAAGTCGATCTACAACTCACTTGATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211053 representing BC032155 Red=Cloning site Green=Tags(s) MADDSVLPSPTEITSLADSSVFDSKVAGMSKENLCLASTSNVDEEMPQVEARVIMVQDAGKQEELLKALK EMKVPCVKMDSMEEFESLDSPEFENIFVVTDFQNSVFNDLYKADCRIVGPPVILNCAQRGEPLPFSCRPL YCTSMLNLVLCFTGFRKKEELVKLVTLVHHMGGVIRKECNSKVTHLVANCTQGEKFRVAVSLGTPIMKPE WIYKAWERRNEQCFCAAVDDFRNEFKVPPFQDCILSFLGFSDEEKHSMEEMTEMQGGSYLPVGDERCTHL IVEENTVKDLPFEPSKKLFVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSLLSLSTPNSNRKRR RLKETLAQLSRETDLSPFPPRKRPSAEHSLSIGSLLDISNTPESSIHYGETPKSCAKSSRSSTPVPPKQS ARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHMKIKDDLED LIANWDESRSIGDIFLKYAKDLVKTYPPFVNFFEMSKEMIIKCEKQKPRFHAFLKINQAKPECGRQSLVE LLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGC PANLLSSHRSLVQRVETVSLGEHPCDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHDRTRPPASLKHIH LMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSEELPKESWLKMLCRHVANTICKADAE NLMYVADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRMALSSSHSSEGRSPPSSGK VAVSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTTHLI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC032155
ORF Size	2648 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032155, AAH32155
RefSeq Size	4012 bp
RefSeq ORF	2648 bp
Locus ID	13605
Gene Summary	Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.[UniProtKB/Swiss-Prot Function]

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