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Eef2 (NM_007907) Mouse Tagged ORF Clone
CAT＃: MG210973
- TrueORF®
Eef2 (tGFP-tagged) - Mouse eukaryotic translation elongation factor 2 (Eef2)
ORF Plasmid: DDK
"NM_007907" in other vectors (4)
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CNY 7,888.00
CNY 9,410.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ef-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210973 representing NM_007907
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAACTTCACAGTAGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCCGGAACATGTCAG TCATCGCCCATGTGGACCACGGCAAGTCCACGCTGACCGACTCCCTTGTGTGCAAGGCTGGCATCATTGC CTCTGCCCGAGCTGGGGAGACGCGCTTCACTGACACTCGCAAGGATGAGCAGGAGCGCTGCATCACAATC AAATCCACCGCCATCTCCCTCTTCTACGAGCTCTCTGAGAACGACCTGAACTTCATTAAGCAGAGCAAGG ATGGCTCGGGCTTCCTCATCAACCTCATCGACTCTCCAGGCCATGTGGACTTCTCTTCAGAGGTGACAGC TGCCTTGCGTGTCACCGATGGAGCTCTGGTGGTGGTGGACTGTGTGTCTGGCGTGTGTGTGCAGACAGAA ACTGTGCTGCGCCAGGCCATCGCTGAACGCATCAAGCCCGTCCTGATGATGAACAAGATGGACCGGGCCC TGCTGGAGCTGCAGCTGGAGCCCGAGGAGCTCTACCAGACCTTCCAGCGCATCGTGGAGAACGTCAACGT CATCATCTCTACCTACGGCGAGGGCGAGAGTGGGCCCATGGGCAATATCATGATTGACCCCGTCCTGGGC ACCGTAGGCTTTGGTTCTGGCCTGCATGGCTGGGCCTTCACCCTGAAGCAGTTTGCGGAGATGTATGTGG CCAAGTTTGCAGCCAAGGGTGAGGGCCAGCTGAGCGCAGCCGAGCGTGCCAAGAAAGTAGAGGACATGAT GAAGAAGCTGTGGGGAGACCGGTACTTTGATCCGGCCAATGGCAAGTTCAGTAAGTCAGCCAATAGCCCA GATGGGAAAAAACTTCCCCGCACCTTCTGCCAGCTCATCCTGGACCCCATCTTCAAGGTGTTCGACGCCA TCATGAACTTCAGGAAGGAGGAGACAGCCAAGCTGATCGAGAAGCTGGACATCAAGCTGGACAGCGAGGA CAAAGACAAGGAGGGCAAGCCATTGCTCAAGGCTGTGATGCGCCGCTGGCTGCCTGCAGGGGACGCCCTC CTGCAGATGATCACCATCCACTTACCATCCCCCGTCACTGCACAGAAGTACCGTTGTGAGCTGCTATACG AGGGGCCTCCCGACGATGAGGCCGCCATGGGTATTAAGAGCTGCGACCCCAAAGGCCCACTTATGATGTA CATTTCCAAGATGGTGCCAACCTCTGACAAAGGCCGCTTCTATGCCTTTGGTAGAGTGTTCTCTGGGGTG GTGTCCACGGGCCTGAAGGTCCGGATCATGGGCCCCAACTACACGCCTGGGAAGAAAGAGGACCTATACC TGAAGCCTATCCAGAGAACCATTCTGATGATGGGCCGCTACGTGGAGCCGATTGAGGACGTGCCATGTGG AAACATTGTGGGGCTGGTCGGAGTGGACCAGTTCCTTGTGAAGACGGGGACCATCACTACCTTTGAGCAC GCTCACAACATGCGCGTGATGAAGTTCAGCGTCAGCCCTGTCGTCCGTGTGGCAGTGGAGGCCAAGAACC CAGCTGACCTGCCCAAGCTGGTGGAGGGGCTGAAGCGGCTGGCTAAGTCTGACCCTATGGTGCAGTGCAT CATTGAGGAGTCTGGAGAACATATTATTGCTGGCGCCGGTGAGCTGCACCTGGAGATCTGCCTTAAGGAC CTGGAGGAGGACCATGCCTGCATCCCCATCAAGAAATCTGACCCTGTTGTGTCATATCGGGAGACAGTCA GTGAGGAATCCAACGTGCTGTGTCTGTCCAAGTCCCCCAATAAGCACAACCGGCTGTACATGAAGGCCAG GCCCTTCCCTGATGGCCTGGCAGAGGACATCGATAAGGGTGAGGTGTCTGCCCGCCAGGAGCTCAAGGCA CGTGCCCGCTACCTGGCCGAAAAGTATGAGTGGGACGTTGCTGAAGCCCGCAAGATCTGGTGCTTTGGCC CTGATGGCACTGGCCCCAACATTCTCACCGACATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGA CAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTGAGGAAAACATGCGTGGTGTG CGGTTTGATGTTCATGATGTGACCCTGCATGCTGATGCCATTCACCGGGGAGGTGGCCAGATCATCCCCA CAGCACGCCGCTGCCTGTATGCCAGTGTGCTGACCGCACAGCCCCGCCTCATGGAGCCTATCTATCTGGT GGAGATCCAGTGTCCTGAGCAAGTGGTGGGTGGCATCTACGGTGTCCTGAACAGGAAGCGTGGCCATGTG TTTGAGGAGTCCCAGGTGGCTGGTACCCCCATGTTTGTGGTCAAGGCATACCTGCCTGTCAATGAGTCCT TTGGCTTCACCGCTGATCTGCGATCCAACACCGGCGGCCAGGCCTTCCCCCAGTGCGTGTTTGACCACTG GCAGATCCTGCCTGGGGATCCTTTTGACAACAGCAGCCGCCCCAGCCAAGTGGTAGCTGAGACGCGCAAG CGCAAGGGCCTGAAAGAGGGCATCCCAGCGCTGGACAACTTCCTGGACAAACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210973 representing NM_007907
Red=Cloning site Green=Tags(s) MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITI KSTAISLFYELSENDLNFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTE TVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLG TVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLSAAERAKKVEDMMKKLWGDRYFDPANGKFSKSANSP DGKKLPRTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDAL LQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGV VSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEH AHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKD LEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKA RARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGV RFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHV FEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRK RKGLKEGIPALDNFLDKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007907 |
| ORF Size | 2574 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007907.2 |
| RefSeq Size | 3111 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 13629 |
| UniProt ID | P58252 |
| Gene Summary | Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200488 | Eef2 (untagged) - Mouse eukaryotic translation elongation factor 2 (Eef2), (10ug) |
CNY 6,288.00 |
|
| MR210973 | Eef2 (Myc-DDK-tagged) - Mouse eukaryotic translation elongation factor 2 (Eef2) |
CNY 8,550.00 |
|
| MR210973L3 | Lenti ORF clone of Eef2 (Myc-DDK-tagged) - Mouse eukaryotic translation elongation factor 2 (Eef2) |
CNY 10,450.00 |
|
| MR210973L4 | Lenti ORF clone of Eef2 (mGFP-tagged) - Mouse eukaryotic translation elongation factor 2 (Eef2) |
CNY 10,450.00 |
