Vav3 (BC052739) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC27838
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210938 representing BC052739 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGTGGAAGCAGTGCGCTCAGTGGCTCATCCACAGCAAGGTGCTGCCCCCCAACCATCGGGTGA CCTGGGACTCGGCGCAGGTGTTCGACCTGGCGCAGACCCTTCGCGATGGAGTCCTGCTCTGCCAGCTGCT CAACAACCTGCGGCCACACTCCATCAATCTGAAGGAGATCAACCTGAGGCCCCAGATGTCCCAGTTTCTC TGTTTGAAGAACATCCGGACATTCCTGGCCGCCTGCTGCGATACCTTTGGAATGAGGAAAAGTGAACTTT TTGAGGCATTTGACCTATTTGATGTTCGAGACTTTGGAAAGGTTATAGAAACATTATCTCGGCTATCCCG AACACCAATAGCATTGGCCACAGGAATCAGGCCGTTCCCCACTGAAGAAAGTATAAATGATGAAGACATC TACAAAGGCCTTCCCGACTTAATAGATGAAACCCGCGTGGAAGATGAAGAAGACCTCTATGACTGTGTGT ACGGCGAGGATGAAGGCGGAGAAGTCTATGAAGACTTGATGAAGGCGGAGGAAGCCCAGCAACCTAAATC ACAAGAAAATGATATACGGAGTTGTTGCCTAGCAGAAATTAGGCAGACAGAAGAAAAATACACAGAAACG TTGGAGTCAATAGAAAAATATTTCATGGCACCATTGAAAAGATTTCTGACGGCGGCGGAATTTGATTCAG TATTTATTAACATTCCTGACCTTGTAAAAGTTCATCGGAGCTTAATGCAAGAGATTCATGATTCCATTGT AAATAAAGATGACCAGAACTTGTATCAAGTTTTTATTAACTACAAGGAAAGATTGGTTATTTATGGGCAG TACTGCAGTGGAGTGGAGTCAGCCATCTCTAACCTAGACTACATCTCTAAGACAAAAGAAGATGTCAAAC TGAAATTAGAGGAATGTTCAAAAAGAGCCAATAACGGGAAGTTTACTCTGCGCGATTTGCTGGTCGTTCC AATGCAGCGCGTGCTGAAGTATCACCTCCTCCTTCAGGAGCTGGTAAAACACACCCATGACCCTATGGAG AAGGCGAACCTGAAGCTGGCTCTGGATGCAATGAAGGACTTGGCACAATATGTGAATGAAGTAAAAAGAG ATAATGAGACCCTTCGTGAAATTAAGCAGTTTCAGCTATCTATAGAGAATTTGAACCAGCCAGTTTTGCT CTTTGGACGACCACAGGGAGACGGTGAAATCCGAATAACCACTCTGGACAAGCATACAAAACAAGAAAGG CATATCTTCTTATTCGACTTGGCAGTGATTGTCTGTAAAAGGAAAGGCGATAACTACGAGATGAAGGAAA TAATAGATCTCCAGCAGTACAAGATAGCCAACAACCCTACCACTGACAAAGAGAATAAAAAGTGGTCTTA TGGCTTCTACCTCATCCATACCCAAGGACAAAATGGGTTAGAATTTTATTGCAAAACAAAAGATTTAAAG AAAAAATGGCTGGAACAGTTTGAAATGGCTCTATCCAACATAAGGCCAGACTATGCAGACTCCAATTTCC ATGATTTCAAGATGCACACCTTCACTCGGGTGACATCCTGCAGGGTCTGCCAGATGCTCCTGAGGGGAAC ATTTTATCAAGGCTACTTATGTTTTAAGTGTGGAGCAAAAGCACATAAAGAATGTTTGGGAAGAGTAGAC AATTGTGGCAGAGTTAATTCCGTTGAACAAGGGCCATTCAAACCACCAGAGAAACGGACCAATGGACTTC GGAGAGCTTCCAGACAGGTGGACCCAGGTTTGCCGAAGATGCAGGTCATTAGGAACTACACTGGCACCCC GGCCCCAGGGCTTCATGAAGGACCACCATTGCATATCCAGGCAGGCGACACTGTTGAACTTCTGAGAGGA GATGCACACAGTGTGTTCTGGCAGGGACGAAACTTAGCTTCAGGAGAGGTTGGATTTTTCCCAAGTGATG CTGTCAAACCCTCTCCATGTGTACCTAAACCAGTAGATTATTCTTGCCAACCCTGGTATGCTGGCCCAAT GGAAAGATTGCAGGCAGAAACTGAACTTATTAACAGGGTGAATAGTACTTACCTTGTAAGGCACAGGACC AAAGAATCTGGAGAATATGCAATTAGCATTAAGTACAATAATGAAGCAAAACACATCAAGATTTTAACAA GAGATGGCTTTTTTCACATTGCAGAAAATAGAAAATTTAAAAGCTTAATGGAACTTGTGGAGTACTACAA GCACCATTCTCTCAAAGAAGGGTTCAGGACCTTAGATACAACGCTACAGTTTCCATACAAGGAGCCAGAA CAGCCAGCTGGACAGAGGGGTAATAGAACAGGCAACAGCTTGTTGAGTCCCAAAGTACTGGGTATTGCCA TCGCTCGGTACGACTTCTGTGCAAGAGATATGCGGGAGCTCTCCCTATTGAAAGGAGACATGGTGAAGAT TTACACGAAGATGAGTGCAAATGGCTGGTGGAGAGGTGAAGTAAATGGCAGGGTGGGCTGGTTTCCATCC ACATATGTGGAAGAAGATGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210938 representing BC052739 Red=Cloning site Green=Tags(s) MEPWKQCAQWLIHSKVLPPNHRVTWDSAQVFDLAQTLRDGVLLCQLLNNLRPHSINLKEINLRPQMSQFL CLKNIRTFLAACCDTFGMRKSELFEAFDLFDVRDFGKVIETLSRLSRTPIALATGIRPFPTEESINDEDI YKGLPDLIDETRVEDEEDLYDCVYGEDEGGEVYEDLMKAEEAQQPKSQENDIRSCCLAEIRQTEEKYTET LESIEKYFMAPLKRFLTAAEFDSVFINIPDLVKVHRSLMQEIHDSIVNKDDQNLYQVFINYKERLVIYGQ YCSGVESAISNLDYISKTKEDVKLKLEECSKRANNGKFTLRDLLVVPMQRVLKYHLLLQELVKHTHDPME KANLKLALDAMKDLAQYVNEVKRDNETLREIKQFQLSIENLNQPVLLFGRPQGDGEIRITTLDKHTKQER HIFLFDLAVIVCKRKGDNYEMKEIIDLQQYKIANNPTTDKENKKWSYGFYLIHTQGQNGLEFYCKTKDLK KKWLEQFEMALSNIRPDYADSNFHDFKMHTFTRVTSCRVCQMLLRGTFYQGYLCFKCGAKAHKECLGRVD NCGRVNSVEQGPFKPPEKRTNGLRRASRQVDPGLPKMQVIRNYTGTPAPGLHEGPPLHIQAGDTVELLRG DAHSVFWQGRNLASGEVGFFPSDAVKPSPCVPKPVDYSCQPWYAGPMERLQAETELINRVNSTYLVRHRT KESGEYAISIKYNNEAKHIKILTRDGFFHIAENRKFKSLMELVEYYKHHSLKEGFRTLDTTLQFPYKEPE QPAGQRGNRTGNSLLSPKVLGIAIARYDFCARDMRELSLLKGDMVKIYTKMSANGWWRGEVNGRVGWFPS TYVEEDE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC052739
ORF Size	2543 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052739, AAH52739
RefSeq Size	4770 bp
RefSeq ORF	2543 bp
Locus ID	57257
Gene Summary	Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases (By similarity). Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3-mediated adhesion. Does not affect integrin beta-1-mediated adhesion.[UniProtKB/Swiss-Prot Function]

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