Pml (BC020990) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1200009E24Rik; AI661194; Trim19
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210910 representing BC020990 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACTGAACCAGTTTCCGTGCAGAAGGTACCTGCACCCCCTGGATCTCCCTGTCGACAACAGGACT CTGCCCTGACCCCCACGCCCACCATGCCTCCCCCAGAGGAACCCTCCGAAGACTATGAACACAGCCAAAG CCCTGCAGAGCAAGCCATACAGGAGGAATTTCAGTTTCTGCGCTGCCCGAGCTGCCAGGCCCAAGCCAAG TGCCCCAAACTGCTGCCTTGCCTGCACACGCTGTGCTCCGGATGCCTGGAGGCGCCTGGCCTGCAGTGCC CCATCTGCAAGGCTCCTGGGCAGGCCGATGCTAATGGGGAGGCCCTGGATAACGTGTTCTTCGAGAGCCT GCAGCGGCGCCTGGCGGTGTTCCGGCAGATCGTGGATGCTCAGGCTGCGTGCACCCGCTGCAAAGGCTTG GCCGACTTCTGGTGTTTCGAGTGTGAACAGCTCATTTGCAGCAAGTGCTTCGAAGCACACCAGTGGTACC TCAAGCATGAGGCCCGGCCCCTGGCCGATCTCCGCGACAATTCAGTGAGCAGCTTCCTCGACAGTACGCG CAAGTCCAATATCTTCTGCTCCAATACCAACCACCGCAACCCTGCGCTGACTGACATCTACTGCCGAGGC TGCGCCAAGCCTCTGTGTTGCACATGCGCGCTCCTGGACCGCAACCACAGCCATCTCCATTGCGATATTG GTGAGGAGATTCAGCAGTGGCATGAGGAACTAGGCACCATGACACAGACTCTGGAGGAGCAGGGCAGAAC CTTCGACAGTGCCCATGCACAGATGTGCTCAGCTATAGGACAGCTGGACCACGCACGCGCAGACATTGAG AAGCAGATCCGCGCACGCGTGCGCCAGGTGGTAGACTACGTGCAGGCTCAGGAGCGCGAGCTGCTCGAGG CGGTGAATGACCGCTACCAGCGCGACTACCAGGAAATAGCTGGCCAGCTGAGCTGCCTGGAAGCTGTGCT GCAGCGCATCCGCACTAGTGGGGCGCTGGTCAAGAGGATGAAGCTCTATGCCTCCGACCAGGAGGTGCTG GATATGCACAGCTTTCTGCGCAAGGCACTCTGTAGCTTGCGCCAGGAGGAGCCCCAGAACCAGAAAGTCC AGCTGCTCACCAGAGGTTTCGAGGAGTTCAAGCTGTGCCTGCAGGACTTCATCTCCTGCATCACCCAGAG GATAAATGCAGCTGTAGCCAGCCCAGAGGCAGCCAGCAATCAACCAGAGGCAGCCAGCACTCACCCAGTG ACAACCAGCACGCCTGAGGACCTTGTGCAGGAGGCTTCTCAGACAGTCGGCTCCATGAAGAGGAAGTGCT CCCACGAAGATTGCTCCAGGAAGATCATCAAGATGGAGTCCACAGAGGAGAACGAGGACAGGTTGGCCAC AAGCTCCCCCGAGCAGTCCTGGCCCAGCACTTTCAAGGCCACCTCCCCTCCCCATCTGGATGGGACTTCC AACCCTGAGAGCACCGTCCCTGAAAAAAAGATCCTCCTGCCCAACAACAATCATGTTACCAGTGACACAG GGGAAACAGAGGAGCGAGTTGTGGTGATCAGCAGCTCAGAGGATTCGGACACCGAGAATCTGTCCTCCCA CGAGTTGGACGATAGCAGCAGTGAGTCCAGCAGCCTGCAGCTGGAGGGCCCCAATTCCCTCAAGGCCTTG GATGAGAGCCTCGCCGAACCCCACTTAGAAGACAGGACCCTGGTTTTCTTTGACCTCAAGATTGACAATG AAACCCAGAAAATTAGCCAGCTGGCCGCGGTGAACCGGGAAAGCAAGTTCCGTGTGCTCATCCAGCCCGA GGCCTTCAGTGTCTACTCCAAAGCTGTCTCCCTGGAGGCAGGGCTCCGGCACTTCCTCAGCTTCCTCACC ACCATGCATCGTCCCATCTTGGCGTGTTCTAGGCTGTGGGGGCCGGGACTCCCCATCTTCTTCCAGACCC TGAGTGATATTAACAAGCTGTGGGAATTCCAGGACACCATCTCAGGTTTCTTGGCCGTGCTGCCCCTCAT CCGGGAACGCATACCCGGCGCTAGCAGCTTCAAACTTGGGAACCTAGCCAAGACCTACCTGGCGAGAAAC ATGAGCGAACGCAGCGCTCTGGCTTCTGTGCTGGCCATGAGGGACTTGTGCTGCCTCCTTGAGATCTCCC CAGGACTGCCGCTGGCCCAGCATATCTACTCCTTTAGTAGCTTGCAGTGTTTTGCTTCCCTGCAGCCCCT AATTCAGGCCAGCGTCCTGCCACAGTCCGAGGCCCGCCTCTTGGCCCTCCACAATGTGAGCTTTGTAGAG TTGCTGAATGCATATCGCACCAACAGGCAAGAAGGCTTGAAGAAGTATGTCCACTATCTGAGCCTGCAGA CCACCCCGTTGTCATCGTCGGCTTCCACCCAAGTTGCCCAATTCCTGCAGGCTCTTAGCACCCACATGGA AGGACTGTTGGAAGGCCATGCCCCTGCTGGGGCAGAAGGCAAAGCTGAAAGTAAGGGATGCCTGGCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210910 representing BC020990 Red=Cloning site Green=Tags(s) METEPVSVQKVPAPPGSPCRQQDSALTPTPTMPPPEEPSEDYEHSQSPAEQAIQEEFQFLRCPSCQAQAK CPKLLPCLHTLCSGCLEAPGLQCPICKAPGQADANGEALDNVFFESLQRRLAVFRQIVDAQAACTRCKGL ADFWCFECEQLICSKCFEAHQWYLKHEARPLADLRDNSVSSFLDSTRKSNIFCSNTNHRNPALTDIYCRG CAKPLCCTCALLDRNHSHLHCDIGEEIQQWHEELGTMTQTLEEQGRTFDSAHAQMCSAIGQLDHARADIE KQIRARVRQVVDYVQAQERELLEAVNDRYQRDYQEIAGQLSCLEAVLQRIRTSGALVKRMKLYASDQEVL DMHSFLRKALCSLRQEEPQNQKVQLLTRGFEEFKLCLQDFISCITQRINAAVASPEAASNQPEAASTHPV TTSTPEDLVQEASQTVGSMKRKCSHEDCSRKIIKMESTEENEDRLATSSPEQSWPSTFKATSPPHLDGTS NPESTVPEKKILLPNNNHVTSDTGETEERVVVISSSEDSDTENLSSHELDDSSSESSSLQLEGPNSLKAL DESLAEPHLEDRTLVFFDLKIDNETQKISQLAAVNRESKFRVLIQPEAFSVYSKAVSLEAGLRHFLSFLT TMHRPILACSRLWGPGLPIFFQTLSDINKLWEFQDTISGFLAVLPLIRERIPGASSFKLGNLAKTYLARN MSERSALASVLAMRDLCCLLEISPGLPLAQHIYSFSSLQCFASLQPLIQASVLPQSEARLLALHNVSFVE LLNAYRTNRQEGLKKYVHYLSLQTTPLSSSASTQVAQFLQALSTHMEGLLEGHAPAGAEGKAESKGCLA SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC020990
ORF Size	2519 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020990, AAH20990
RefSeq Size	4296 bp
RefSeq ORF	2519 bp
Locus ID	18854
Gene Summary	Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. Positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum. Regulates RB1 phosphorylation and activity. Acts as both a negative regulator of PPARGC1A acetylation and a potent activator of PPAR signaling and fatty acid oxidation. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates PER2 nuclear localization and circadian function. Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway. Required for normal development of the brain cortex during embryogenesis. Plays a role in granulopoiesis or monopoiesis of myeloid progenitor cells. May play a role regulating stem and progenitor cell fate in tissues as diverse as blood, brain and breast. Shows antiviral activity towards lymphocytic choriomeningitis virus (LCMV) and the vesicular stomatitis virus (VSV).[UniProtKB/Swiss-Prot Function]

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