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Atp6v0a1 (BC071182) Mouse Tagged ORF Clone
CAT＃: MG210870
- TrueORF®
Atp6v0a1 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184)
ORF Plasmid: DDK
"BC071182" in other vectors (4)
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CNY 5,230.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ATP6a1, Atpv0a1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210870 representing BC071182
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAGCTTTTCCGGAGTGAGGAGATGACGCTGGCCCAGCTATTTCTACAGTCTGAAGCTGCTTATT GCTGTGTCAGTGAATTAGGAGAGCTTGGGAAGGTCCAATTTCGGGATTTAAATCCAGATGTGAATGTTTT CCAGAGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAAATGGATCGAAAACTCCGATTTGTTGAGAAA GAGATAAGGAAAGCTAACATCCCAATTATGGACACCGGTGAAAACCCAGAGGTGCCCTTCCCTCGGGACA TGATCGACTTAGAGGCCAATTTCGAGAAGATTGAAAATGAACTGAAGGAAATCAACACTAACCAGGAAGC TCTGAAGAGAAACTTCCTGGAACTGACTGAATTAAAATTTATCCTGCGAAAAACCCAGCAGTTTTTCGAT GAGATGGCGGATCCAGACCTGTTGGAAGAGTCCTCATCACTCTTGGAGCCAAACGAGATGGGAAGAGGCG CACCCTTACGACTTGGCTTTGTGGCTGGTGTGATTAACCGGGAGCGGATCCCGACCTTTGAGCGCATGCT TTGGCGAGTGTGCCGAGGGAATGTGTTCCTGAGACAAGCTGAAATCGAAAACCCGCTGGAGGATCCTGTG ACTGGCGACTACGTGCACAAGTCTGTGTTCATCATTTTTTTCCAAGGCGATCAGCTGAAAAACAGGGTCA AGAAAATCTGTGAAGGGTTCCGAGCCTCCCTCTATCCCTGCCCTGAGACTCCACAGGAAAGGAAAGAAAT GGCTTCCGGAGTCAATACCAGGATCGACGACCTCCAAATGGTTCTGAATCAGACGGAGGACCACCGCCAG AGGGTTCTGCAGGCAGCTGCTAAGAACATCCGCGTCTGGTTCATCAAGGTGCGGAAGATGAAGGCTATCT ACCATACCCTGAACCTGTGCAATATAGACGTGACCCAGAAGTGCCTGATCGCAGAAGTCTGGTGCCCTGT CACTGACCTGGACTCCATCCAGTTTGCACTACGAAGAGGCACGGAACACAGTGGGTCCACTGTCCCCTCC ATTCTGAACAGGATGCAGACAAACCAGACACCGCCGACATATAATAAAACCAACAAGTTCACGCACGGCT TCCAGAACATAGTAGATGCTTATGGGATCGGCACTTACCGAGAGATTAACCCAGCTCCGTACACTGTCAT CACCTTCCCCTTTCTGTTTGCTGTGATGTTCGGAGATTTTGGGCACGGCATTTTGATGACGCTGTTTGCT GTGTGGATGGTGTTGAGGGAGAGCCGGATCCTCTCCCAGAAGCACGAGAATGAGATGTTTAGCATGGTGT TCAGCGGCCGGTACATTATTCTTCTGATGGGACTGTTCTCCATCTATACTGGACTCATCTACAATGACTG CTTTTCCAAGTCTCTGAATATCTTTGGGTCATCGTGGAGTGTACGGCCAATGTTCACTCAAGGGAACTGG ACGGAGGAGACGCTTCTGGGGAGTTCTGTTCTCCAGTTGAACCCCGCTATCCCTGGAGTTTTTGGTGGCC CGTACCCGTTTGGCATTGATCCGATTTGGAACATTGCAACCAACAAGCTGACCTTCCTCAACTCCTTCAA GATGAAGATGTCTGTTATCCTCGGCATCATCCACATGCTGTTTGGAGTCAGCCTGAGCCTTTTCAACCAT ATCTATTTCAAGAAGCCCCTGAACATCTACTTTGGCTTTATTCCTGAGATCATCTTCATGTCCTCGTTGT TTGGCTACCTGGTCATCCTTATCTTTTACAAGTGGACAGCCTACGATGCCCACTCGTCTAGGAATGCCCC GAGCCTCCTGACCCACTTCATAAACATGTTCCTCTTCTCCTACCCAGAGTCTGGTAATGCAATGCTGTAC TCTGGACAGAAAGGAATTCAGTGTTTCCTCATCGTGGTGGCGATGCTCTGCGTCCCTTGGATGCTTCTGT TTAAGCCGCTGATCCTTCGCCATCAGTACCTGAGGAAGAAGCATTTGGGAACTCTCAACTTTGGTGGGAT CAGGGTGGGCAACGGACCGACAGAGGAGGATGCTGAAATTATTCAGCATGACCAGCTTTCCACCCATTCA GAGGACGCAGAAGAGTTTGACTTTGGCGACACCATGGTCCACCAGGCCATCCACACCATTGAGTACTGCT TGGGTTGTATCTCCAACACCACGTCGTACCTGCGACTCTGGGCCCTCAGCCTGGCCCACGCACAGCTCTC TGAAGTACTTTGGACCATGGTGATCCACATTGGCCTGCACGTCCGGAGTTTGGCCGGGGGTCTAGGGCTG TTTTTCATCTTCGCCGCCTTTGCCACGCTGACCGTGGCTATCCTGCTGATCATGGAGGGCCTCTCGGCCT TCCTCCACGCGCTGCGGTTACACTGGGTGGAGTTCCAGAACAAATTCTACACTGGGACTGGCTTCAAGTT CCTCCCCTTCTCCTTTGAGCACATTCGGGAAGGGAAGTTTGATGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210870 representing BC071182
Red=Cloning site Green=Tags(s) MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEK EIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFD EMADPDLLEESSSLLEPNEMGRGAPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPV TGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQ RVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPS ILNRMQTNQTPPTYNKTNKFTHGFQNIVDAYGIGTYREINPAPYTVITFPFLFAVMFGDFGHGILMTLFA VWMVLRESRILSQKHENEMFSMVFSGRYIILLMGLFSIYTGLIYNDCFSKSLNIFGSSWSVRPMFTQGNW TEETLLGSSVLQLNPAIPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHMLFGVSLSLFNH IYFKKPLNIYFGFIPEIIFMSSLFGYLVILIFYKWTAYDAHSSRNAPSLLTHFINMFLFSYPESGNAMLY SGQKGIQCFLIVVAMLCVPWMLLFKPLILRHQYLRKKHLGTLNFGGIRVGNGPTEEDAEIIQHDQLSTHS EDAEEFDFGDTMVHQAIHTIEYCLGCISNTTSYLRLWALSLAHAQLSEVLWTMVIHIGLHVRSLAGGLGL FFIFAAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYTGTGFKFLPFSFEHIREGKFDE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC071182 |
| ORF Size | 2496 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC071182.1 |
| RefSeq Size | 3980 bp |
| RefSeq ORF | 2498 bp |
| Locus ID | 11975 |
| Gene Summary | Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220706 | Atp6v0a1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184), (10ug) |
CNY 9,120.00 |
|
| MR210870 | Atp6v0a1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184) |
CNY 6,096.00 |
|
| MR210870L3 | Lenti ORF clone of Atp6v0a1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184) |
CNY 6,650.00 |
|
| MR210870L4 | Lenti ORF clone of Atp6v0a1 (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184) |
CNY 6,650.00 |
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