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Ptchd2 (BC070441) Mouse Tagged ORF Clone
CAT＃: MG210841
- TrueORF®
Ptchd2 (tGFP-tagged) - Mouse patched domain containing 2 (cDNA clone MGC:99871 IMAGE:30535318)
ORF Plasmid: DDK
"BC070441" in other vectors (4)
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CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Disp3; G630052C06Rik; RNDEu-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210841 representing BC070441
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCAGAGGATGATCCCCTGCTGCAGGATGTGTGGCTAGAAGAGGAACAGCCGGAAGATGAAGCCT GCAGGGGTATCCCCGGGCCAGGGCTGCAGTCCGGAGCACAGGGCTGCTGGCGACGCTGGACACTGCCTTC CAGACCCCCGACTTTGGGGTTCTGGAGCACTCTGGGCTGGGCCTTCACCAACCCGTGCTGCGCAGGGCTG GTGCTGTTCCTTGGCTGCAGCATCCCCATGGTGCTGTCTGCCTTCATGTTCCTCTACTACCCGCCCCTGG ACATCGACATCTCCTACAACGCCTTTGAGATCCGCAACCACGAGGCATCACAGCGCTTTGACGCCCTGGC CTTGGCCCTCAAGTCCCAGTTTGGATCTTGGGGGCGCAACAGGCGCGACCTGGCGGACTTCACCTCCGAG ACGTTGCAGCGCCTCATCTCAGAGCAGCTTCAGCAGCTGCACCTGGGGAATCATTCTCGACCTGCATCCC GAGCTCCTCGCTCAGCACCGAGGGACACTGTTGCCACTCAGACATCAGCAGCCAATTCGAGTGAGCGACG TCGCCGGGAGGCTCCGTCCCCGGAGGGTCAGGTAACCAATCAGAGCCGGGCCCGTCGGGGCGCCTCGCGC TGGGATTACTCGCGCACCTATGTGAGCGCCAACACCCAAACACACGCGCATTGGCGAATCGAGCTCATCT TTCTGGCTCGCGGAGACGCGGAGCGCAACATTTTCACGAGCGAGCGTCTAGTCACGATCCACGAGATCGA GCGTAAAATCATGGACCACCCGGGTTTCCGGGAGTTCTGCTGGAAGCCCCATGAGGTGCTCAAGGACCTG CCGCTCGGATCCTATTCCTACTGCTCCCCGCCTAGTTCTCTCATGACTTACTTTTTCCCCACCGAGAGGG GCGGCAAGATCTACTACGACGGCATGGGCCAGGACCTGGCGGATATCCGGGGCTCCCTAGAACTGGCCAT GACTCACCCTGAGTTCTACTGGTATGTGGATGAGGGGCTCTCCGTGGATAATCTGAAGAGCTCCCTCCTG CGCAGTGAGATCCTGTTTGGAGCGCCGCTTCCCAACTACTACTCAGTGGATGATCGCTGGGAGGAGCAGA GAGCCAAGTTTCAGAGCTTCGTAGTCACGTATGTGGCCATGCTCGCCAAGCAGTCCACTAGCAAAGTCCA GGTCCTCTATGGGGGGACAGACCTCTTCGACTATGAGGTACGCCGAACCTTCAATAACGACATGCTCCTT GCCTTCATTAGCAGTAGCTGCATCGCGGCCCTCGTCTACATCCTAACCTCCTGCTCAGTGTTCCTGTCCT TCTTTGGGATCGCCAGCATCGGTCTCAGCTGCCTGGTGGCACTCTTTCTCTACCACGTGGTCTTTGGCAT CCAGTACTTGGGCATCCTCAATGGAGTGGCCGCCTTTGTGATTGTGGGCATTGGTGTGGACGATGTCTTT GTATTCATCAACACTTACCGCCAGGCCACCCACCTGGAAGACCCCCAACTGCGGATGATCCACACCATCC AGACTGCAGGCAAAGCCACCTTCTTCACCTCGCTGACCACCGCCGCCGCTTATGCGGCCAACGTCTTCTC CCAGATCCCAGCTGTGCATGACTTCGGCTTGTTCATGTCCCTCATTGTGACCTGCTGCTGGCTGGCTGTG CTGTTCACCATGCCTGCGGCCCTGGGCCTCTGGAGCCTCTACATGGCGCCCCTGGAGAGCTCCTGCCAGA ACAGCTGCCACCAGAAGTGCGGCCGCAAGAGCTCCTTGCATTTTCCTGGGGACCTGTTCACGGCTCCGGA GCGGGCTGGGGGTGGCCCTGCTCAGGGTCCCCTCCCCTATCTGGATGATGATATCCCCTTGTTGAATGTG GAGGATGAGCCAGCGTCCCTAGAGCTGGGAGATGTGTCACTGGTCTCTGTGCACTGTGAGGGTTTGCAGC CCACCCCTGATGCAAACAGCAGGGGCCAGCTCCTCGCCCAGCTGCAAGAGCTGCTGCACCACTGGGTCTT ATGGGCAGCCGTCAAGAGCCGCTGGGTGATCGTAGGGCTTTTCGCCTCCATCCTTATCCTGTCCCTGGTG TTCGCCAGCCGGCTCCGCCCTGCCAGCCGGGCCCCACTCCTCTTCCGGCCGGACACCAACATTCAGGTGC TACTGGACCTCAAGTACAACCTGAGCGCCGAGGGGATATCCTGCATCACCTGCTCAGGTCTGTTCCAGGA GAAGCCCCACAGCCTGCAGAACAATGTCAGGACATCCCTGGAGAAGAAGAAGCGAGGTTCTGGGGTTTCC TGGGCCAGCCGCACTGAGACCACTGCACAGGAGTCCATGAGCACCGTGTACATCTCCAAAGTGAAGAGCA AAGGCCACCCAGCGGTCTACCGGCTCTCTCTCAATGCCAGCCTACCAGCACCCTGGCAAGCTGTGTCCCC TGGAGATGGAGAGGTGCCCTCCTTCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210841 representing BC070441
Red=Cloning site Green=Tags(s) MDSEDDPLLQDVWLEEEQPEDEACRGIPGPGLQSGAQGCWRRWTLPSRPPTLGFWSTLGWAFTNPCCAGL VLFLGCSIPMVLSAFMFLYYPPLDIDISYNAFEIRNHEASQRFDALALALKSQFGSWGRNRRDLADFTSE TLQRLISEQLQQLHLGNHSRPASRAPRSAPRDTVATQTSAANSSERRRREAPSPEGQVTNQSRARRGASR WDYSRTYVSANTQTHAHWRIELIFLARGDAERNIFTSERLVTIHEIERKIMDHPGFREFCWKPHEVLKDL PLGSYSYCSPPSSLMTYFFPTERGGKIYYDGMGQDLADIRGSLELAMTHPEFYWYVDEGLSVDNLKSSLL RSEILFGAPLPNYYSVDDRWEEQRAKFQSFVVTYVAMLAKQSTSKVQVLYGGTDLFDYEVRRTFNNDMLL AFISSSCIAALVYILTSCSVFLSFFGIASIGLSCLVALFLYHVVFGIQYLGILNGVAAFVIVGIGVDDVF VFINTYRQATHLEDPQLRMIHTIQTAGKATFFTSLTTAAAYAANVFSQIPAVHDFGLFMSLIVTCCWLAV LFTMPAALGLWSLYMAPLESSCQNSCHQKCGRKSSLHFPGDLFTAPERAGGGPAQGPLPYLDDDIPLLNV EDEPASLELGDVSLVSVHCEGLQPTPDANSRGQLLAQLQELLHHWVLWAAVKSRWVIVGLFASILILSLV FASRLRPASRAPLLFRPDTNIQVLLDLKYNLSAEGISCITCSGLFQEKPHSLQNNVRTSLEKKKRGSGVS WASRTETTAQESMSTVYISKVKSKGHPAVYRLSLNASLPAPWQAVSPGDGEVPSFQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC070441 |
| ORF Size | 2478 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC070441.1 |
| RefSeq Size | 5174 bp |
| RefSeq ORF | 2480 bp |
| Locus ID | 242748 |
| Gene Summary | Plays a role in neuronal proliferation and differentiation. Plays a role in the accumulation of cellular cholesterol. Involved in intracellular lipid droplet formation. May contribute to cholesterol homeostasis in neuronal cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220928 | Ptchd2 (untagged) - Mouse patched domain containing 2 (cDNA clone MGC:99871 IMAGE:30535318), (10ug) |
CNY 9,120.00 |
|
| MR210841 | Ptchd2 (Myc-DDK-tagged) - Mouse patched domain containing 2 (cDNA clone MGC:99871 IMAGE:30535318) |
CNY 8,270.00 |
|
| MR210841L3 | Lenti ORF clone of Ptchd2 (Myc-DDK-tagged) - Mouse patched domain containing 2 (cDNA clone MGC:99871 IMAGE:30535318) |
CNY 10,170.00 |
|
| MR210841L4 | Lenti ORF clone of Ptchd2 (mGFP-tagged) - Mouse patched domain containing 2 (cDNA clone MGC:99871 IMAGE:30535318) |
CNY 10,170.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
