United States
Germany
Japan
United Kingdom
China
Usp16 (NM_024258) Mouse Tagged ORF Clone
CAT＃: MG210833
- TrueORF®
Usp16 (tGFP-tagged) - Mouse ubiquitin specific peptidase 16 (Usp16)
ORF Plasmid: DDK
"NM_024258" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1200004E02Rik; 2810483I07Rik; 6330514E22Rik; UBPM |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210833 representing NM_024258
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGAAACGGACCAAGGGGAGAAGTGCTCCAGACACGGTGGCCTCAGAGTCTGCAGAACCAGTGT GCAGACACCTTAGAAAAGGGTTGGAACAAGGTAATTTGAAAAAAGCTTTAGTAAATGTGGAGTGGAATAT CTGCCAAGACTGTAAGACTGACAATAAAGTGAAAGATAAACCTGAGGAGGAAGCAGAAGACCCTTCGGTT TGGCTCTGTCTTAAATGTGGCCATCAGGGCTGTGGCAGAGATTCTCAGGAGCAGCATGCCTTGAAGCACT ACACGACACCGAGATCCGAGCCTCACTACCTGGTGCTCAGTCTGGACAACTGGAGCGTCTGGTGCTACAA GTGTGACGAGGAAGTCAAGTACTGTAGCTCAAACCGATTGGGCCAAGTGGTTGATTATGTTAGAAAACAA GCTGGCGTAAGAACTTCAAAACCAGCAGAGAAAAATAATGGACACATTGAGCTCGAAAATAAAAAATTGG AGAAAGAGAGTAAAAATGAACAAGAGAAAGAGAAATCGGAAAACCTGGCTAAAGAAACTATTCCCATGGA CTCTGCTTCCCAGATAACTGTGAAAGGACTCAGTAATTTGGGGAATACTTGTTTCTTCAATGCAGTTATG CAGAACTTGTCACAAACGCCAGTGCTTAGAGAACTACTAAAAGAAGTGAAGATGTCTGGAACGATTGTAA AAATAGAGCCACCTGATCTGGCACTAACAGAACCTTTAGAAGTAAACCTCGAGCCTCCAGGTCCTCTTAC TTTAGCCATGAGCCAGTTTCTCAGTGAGATGCAAGAGAACAAAAAGCGAGTTGTGACACCTAAAGAGCTC TTTTCTCAGGTCTGTAAAAAAGCAACACGTTTTAAAGGGTACCAGCAACAAGACAGCCAGGAGCTGCTTC GCTACCTACTGGATGGGATGAGAGCGGAAGAACACCAAAGAGTGAGTAAAGGAATTCTTAAAGCATTTGG TAATTCTACTGAAAAATTGGATGAAGAAGTAAAAAATAAAGTTAAAGATTATGAAAAGAAAAAGGCAATC CCGAGTTTTGTGGACCGCATCTTTGGTGGCGAGCTGACTAGTACGATCATGTGTGATGAATGCAGGACTG TCTCCTTAGTGCATGAATCGTTCCTTGATTTGTCTCTTCCAGTTTTAGATGATCAGAGTGGTAAGAAAAG TATAAATGATAAAAATGTGAAAATGACAATGGAGGAAGAAGATAAAGACAGTGAGGAAGAGAAAGATGAC AGCTACATGAAATCAAGGAGCGATCTTCCGTCAGGGACAAGCAAGCACCTACAGAAAAAGGCAAAGAAGC AGGCCAAAAAGCAGGCCAAGAACCAACGAAGGCAACAAAAAATTCAAGAAAGATTTCTTCACTTCAATGA GCTCTGCGCCACTGACTACACGGAAGACAATGAACGTGAAGCTGACACAGCACTTGCGGGAGAAGTGGAA GTGGATACCGACTCCACCCATGGTTCTCAAGAGGAGGCCACACAGATAGAGCTGTCTGTTAACCAGAAGG ATTTGGATGGCCAAGAGAGCATGATAGAAAGGACACCTGATGTGCAGGAAAGCCCAGAGGACCTAGGAGT GAAAAGTGCTAACACCGAGAGTGATCTGGGGATTGTGACGCCTGCTCCTGAATGTCCTAGGGATTTCAAT GGTGCCTTCCTGGAAGAAAGGACCAGTGGAGAACTAGACATTATCAATGGTTTAAAAAACCTTAATTTGA ATGCTGCTGTTGATCCTGATGAAATAAATATAGAGATTCCGAATGACAGTCATTCTGCACCCAAGGTATA TGAGGTCATGAACGAGGACCCAGAAACTGCTTTCTGTACCCTCGCGAACCGAGAAGCGTTTAGTACTGAT GAGTGTTCCATTCAACATTGCTTATATCAGTTCACCCGGAATGAGAAACTTCAAGATGCCAATAAACTGC TTTGTGAAGTGTGTTCAAGACGGCAGTGTAATGGACCAAAGGCAAATATAAAAGGTGACAGGAGACATGT TTACACCAATGCCAAGAAGCAGATGCTGGTCTCCCTCGCGCCTCCTGTCCTCACTCTGCATTTAAAGCGA TTCCAGCAGGCTGGTTTTAACCTGCGCAAAGTTAACAAACACATAAAGTTTCCAGAAATCTTAGATTTGG CTCCTTTTTGTACCCTTAAATGTAAGAATGTTGCTGAAGAAAGTACACGAGTGCTGTATTCCTTATATGG AGTTGTTGAACACAGTGGTACTATGAGGTCAGGGCATTACACTGCCTATGCGAAGGAGAGAACTGCAAGC TGTCACCTCTCCAATCTTGTTCTTCACGGTGACATTCCACAAGATTGTGAAATGGAATCAACCAAAGGGC AGTGGTTTCACATCAGCGATACACATGTGCAAGCTGTGCCTATAACTAAAGTACTGAACTCACAAGCATA TCTCCTATTTTATGAGAGAATACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210833 representing NM_024258
Red=Cloning site Green=Tags(s) MGKKRTKGRSAPDTVASESAEPVCRHLRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKPEEEAEDPSV WLCLKCGHQGCGRDSQEQHALKHYTTPRSEPHYLVLSLDNWSVWCYKCDEEVKYCSSNRLGQVVDYVRKQ AGVRTSKPAEKNNGHIELENKKLEKESKNEQEKEKSENLAKETIPMDSASQITVKGLSNLGNTCFFNAVM QNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEVNLEPPGPLTLAMSQFLSEMQENKKRVVTPKEL FSQVCKKATRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEEVKNKVKDYEKKKAI PSFVDRIFGGELTSTIMCDECRTVSLVHESFLDLSLPVLDDQSGKKSINDKNVKMTMEEEDKDSEEEKDD SYMKSRSDLPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQERFLHFNELCATDYTEDNEREADTALAGEVE VDTDSTHGSQEEATQIELSVNQKDLDGQESMIERTPDVQESPEDLGVKSANTESDLGIVTPAPECPRDFN GAFLEERTSGELDIINGLKNLNLNAAVDPDEINIEIPNDSHSAPKVYEVMNEDPETAFCTLANREAFSTD ECSIQHCLYQFTRNEKLQDANKLLCEVCSRRQCNGPKANIKGDRRHVYTNAKKQMLVSLAPPVLTLHLKR FQQAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEESTRVLYSLYGVVEHSGTMRSGHYTAYAKERTAS CHLSNLVLHGDIPQDCEMESTKGQWFHISDTHVQAVPITKVLNSQAYLLFYERIL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024258 |
| ORF Size | 2475 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024258.1, NP_077220.1 |
| RefSeq Size | 2926 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 74112 |
| UniProt ID | Q99LG0 |
| Gene Summary | Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes (PubMed:24034696). Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221839 | Usp16 (untagged) - Mouse ubiquitin specific peptidase 16 (Usp16), (10ug) |
CNY 10,550.00 |
|
| MR210833 | Usp16 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 16 (Usp16) |
CNY 6,048.00 |
|
| MR210833L3 | Lenti ORF clone of Usp16 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 16 (Usp16) |
CNY 7,700.00 |
|
| MR210833L4 | Lenti ORF clone of Usp16 (mGFP-tagged) - Mouse ubiquitin specific peptidase 16 (Usp16) |
CNY 7,700.00 |
