Tlr6 (BC055366) Mouse Tagged ORF Clone

CAT#: MG210761

  • TrueORF®

Tlr6 (tGFP-tagged) - Mouse toll-like receptor 6 (cDNA clone MGC:60565 IMAGE:30056593)



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Mouse Protein / Lysate Request

CNY 6,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210761 representing BC055366
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAAAGTCCCTCTGGGATAGCCTCTGCAACATGAGCCAAGACAGAAAACCCATCGTGGGGAGTTTCC
ACTTTGTTTGCGCCCTGGCCTTAATAGTCGGAAGCATGACCCCGTTCTCTAATGAACTTGAGTCTATGGT
AGACTATTCAAACAGGAACCTTACTCATGTCCCCAAAGACCTGCCACCAAGAACAAAAGCCCTGAGTCTG
TCTCAAAACTCTATATCTGAGCTTCGGATGCCTGATATCAGCTTTCTGTCAGAGCTGAGAGTTCTGAGAC
TCTCCCACAACAGGATACGGAGCCTTGATTTCCATGTATTCTTGTTCAATCAGGACTTAGAATACCTGGA
TGTCTCACACAATCGGTTGCAAAACATCTCTTGCTGCCCTATGGCGAGCCTGAGGCATCTAGACCTCTCA
TTCAATGACTTTGATGTACTGCCTGTGTGTAAGGAATTTGGCAACCTGACGAAGCTGACTTTCCTGGGAT
TAAGTGCTGCAAAGTTCCGACAACTGGATCTGCTCCCAGTTGCTCACTTGCATCTAAGCTGCATTCTTCT
GGACTTAGTGAGTTATCATATAAAAGGCGGGGAAACAGAAAGTCTTCAGATTCCCAATACCACCGTTCTC
CATTTGGTCTTTCATCCAAATAGCTTGTTCTCTGTTCAAGTGAACATGTCTGTAAACGCTTTAGGACATT
TACAACTGAGTAATATTAAATTGAATGATGAAAACTGTCAAAGGTTAATGACATTTTTATCAGAACTCAC
CAGAGGTCCAACCTTATTGAATGTGACCCTCCAGCACATAGAAACAACCTGGAAGTGCTCGGTTAAACTT
TTCCAATTCTTTTGGCCCCGACCGGTGGAGTACCTCAATATTTACAACTTAACGATAACTGAGAGAATCG
ACAGGGAAGAATTTACTTACTCGGAGACAGCACTGAAGTCACTGATGATAGAGCACGTCAAAAACCAAGT
GTTCCTCTTTTCAAAGGAGGCGCTATACTCGGTGTTTGCTGAGATGAACATCAAGATGCTCTCTATCTCA
GACACCCCTTTCATCCACATGGTGTGCCCGCCATCCCCAAGCTCATTTACATTTCTGAACTTTACCCAGA
ATGTTTTTACTGACAGTGTTTTTCAAGGCTGTTCCACCTTAAAGAGATTGCAGACACTTATCTTACAAAG
GAATGGTTTGAAGAACTTTTTTAAAGTAGCTCTCATGACTAAGAATATGTCCTCTCTGGAAACTTTGGAT
GTTAGTTTGAATTCTTTGAACTCTCATGCATATGACAGGACATGCGCCTGGGCTGAGAGCATATTGGTGT
TGAATTTGTCTTCGAATATGCTTACAGGCTCTGTCTTCAGATGCTTACCTCCCAAGGTCAAGGTCCTTGA
CCTTCACAACAACAGGATAATGAGCATCCCTAAAGATGTCACCCACCTGCAGGCTTTGCAGGAACTCAAT
GTAGCATCCAACTCCTTAACTGACCTTCCTGGGTGTGGGGCCTTCAGCAGCCTTTCTGTGCTGGTCATCG
ACCATAACTCAGTTTCCCATCCCTCTGAGGATTTCTTCCAGAGCTGTCAGAATATTAGATCCCTAACAGC
GGGAAACAACCCATTCCAATGCACATGTGAGCTGAGGGACTTTGTCAAGAACATAGGCTGGGTAGCAAGA
GAAGTGGTGGAGGGCTGGCCTGACTCTTACAGGTGTGACTACCCAGAAAGCTCTAAGGGAACTGCACTGA
GGGACTTCCACATGTCTCCACTGTCCTGTGATACTGTTCTGCTGACTATCACCATCGGGGCCACTATGCT
GGTGCTGGCTGTCACTGGGGCTTTCCTCTGTCTCTACTTTGACCTGCCCTGGTATGTGAGGATGCTGTGT
CAGTGGACACAGACCAGGCACAGGGCCAGGCACATCCCCTTAGAGGAACTCCAGAGAAACCTCCAGTTCC
ATGCTTTTGTCTCATACAGTGAGCATGATTCTGCCTGGGTGAAGAACGAATTACTACCCAACCTAGAGAA
AGATGACATCCGGGTTTGCCTCCATGAGAGGAACTTTGTCCCTGGCAAGAGCATTGTGGAGAACATCATC
AATTTCATTGAGAAGAGTTACAAGGCCATCTTTGTGCTGTCTCCCCACTTCATCCAGAGTGAGTGGTGCC
ATTATGAACTCTATTTTGCCCATCATAATCTCTTCCATGAAGGCTCTGATAACTTAATCCTCATCTTGCT
GGAACCCATTCTACAGAACAACATTCCCAGTAGATACCACAAGCTGCGGGCTCTCATGGCACAGCGGACT
TACTTGGAATGGCCTACTGAGAAGGGCAAACGTGGGCTGTTTTGGGCCAACCTTAGAGCTTCATTTATTA
TGAAGTTAGCCTTAGTCAATGAGGATGATGTGAAAACT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210761 representing BC055366
Red=Cloning site Green=Tags(s)

MVKSLWDSLCNMSQDRKPIVGSFHFVCALALIVGSMTPFSNELESMVDYSNRNLTHVPKDLPPRTKALSL
SQNSISELRMPDISFLSELRVLRLSHNRIRSLDFHVFLFNQDLEYLDVSHNRLQNISCCPMASLRHLDLS
FNDFDVLPVCKEFGNLTKLTFLGLSAAKFRQLDLLPVAHLHLSCILLDLVSYHIKGGETESLQIPNTTVL
HLVFHPNSLFSVQVNMSVNALGHLQLSNIKLNDENCQRLMTFLSELTRGPTLLNVTLQHIETTWKCSVKL
FQFFWPRPVEYLNIYNLTITERIDREEFTYSETALKSLMIEHVKNQVFLFSKEALYSVFAEMNIKMLSIS
DTPFIHMVCPPSPSSFTFLNFTQNVFTDSVFQGCSTLKRLQTLILQRNGLKNFFKVALMTKNMSSLETLD
VSLNSLNSHAYDRTCAWAESILVLNLSSNMLTGSVFRCLPPKVKVLDLHNNRIMSIPKDVTHLQALQELN
VASNSLTDLPGCGAFSSLSVLVIDHNSVSHPSEDFFQSCQNIRSLTAGNNPFQCTCELRDFVKNIGWVAR
EVVEGWPDSYRCDYPESSKGTALRDFHMSPLSCDTVLLTITIGATMLVLAVTGAFLCLYFDLPWYVRMLC
QWTQTRHRARHIPLEELQRNLQFHAFVSYSEHDSAWVKNELLPNLEKDDIRVCLHERNFVPGKSIVENII
NFIEKSYKAIFVLSPHFIQSEWCHYELYFAHHNLFHEGSDNLILILLEPILQNNIPSRYHKLRALMAQRT
YLEWPTEKGKRGLFWANLRASFIMKLALVNEDDVKT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC055366
ORF Size 2420 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC055366, AAH55366
RefSeq Size 2625 bp
RefSeq ORF 2420 bp
Locus ID 21899
Gene Summary Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides (PubMed:19931471). In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion (PubMed:20037584, PubMed:23812099).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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