H6pd (BC042677) Mouse Tagged ORF Clone

CAT#: MG210665

  • TrueORF®

H6pd (tGFP-tagged) - Mouse hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (cDNA clone MGC:25861 IMAGE:4195793)

ORF Plasmid: DDK tGFP



  "BC042677" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,650.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI785303; G6pd1; Gpd-1; Gpd1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210665 representing BC042677
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCTAGCGGCAATGTGCTTGGCCCTTCTGGGCTGCCTACAGGCGCAGGAACTCAAGGGTCATGTTT
CCATAATCCTGCTGGGAGCAACCGGGGACCTAGCTAAAAAGTACCTGTGGCAGGGACTGTTTCAGCTATA
CCTGGATGAGGCTGGGAAGGGCCACAGTTTCAGCTTCCACGGTGCTGCCCTGACAGCCCCCCAGCAAGGC
CAGAAGCTCATGGACAAGGCCTTGGAATCCCTCTCCTGTCCCAAGGACTTGGTACCCAGTCGCTGTGATG
AACTCAAGGGTCAGTTTCTACAGCTGAGCCAATACCACCAGCTGAAGACAGTGGAGGACTATCAGACCCT
CAACAAGGATATTGAGACCCAGGTCCAGCAGGATGGGCTCTGGGAGGCAGGCAGGATCTTCTACTTCAGT
GTGCCGCCCTTTGCCTACGCAGACATTGCCCGTAACATCAACAGCAGCTGCCGACCACACCCAGGTGCCT
GGCTACGGGTTGTTTTTGAAAAACCCTTTGGCCATGACCACCTCTCAGCCCAGCAGCTGGCTTCAGAACT
TGGAAGCTTTTTCCAGGAGGAGGAGATGTACCGTGTGGATCATTACCTCGGCAAGCAGGCGGTGGCTCAG
ATCCTGCCCTTCCGAGATCAGAACCGCAAGGCCTTGGATGGCCTCTGGAACAGGCACCATGTGGAGCGGG
TGGAGATCATTTTGAAGGAGACCATAGATGCGGAAGGTCGGGCCAGTTTCTATGAGGAGTATGGCGTCAT
CCGTGACACGCTGCAGAACCACCTGACTGAGATCCTCACGCTGGTGGCCATGGAACTGCCTCTCAACATC
AGCAGCTCAGCCACCGTGCTCCAGCATAAGCTCTGGGCCTTCCAGGCCCTGCGGGGGCTGCAGAAGAGCA
GTGCCATCCTGGGCCAGTACCAGGCCTACAGCGGACAGGTGCGCCGAGAGCTGCAGAAGCCAGACGGCTT
CCAGAGCCTGACACCAACCTTCGCAGGTGTCCTTGTCCACATCGACAACCTGCGTTGGGAGGGTGTCCCT
TTCATCCTGATGTCTGGCAAGGCCTTGGATGAGAGAGTGGGCTATGTTCGGATTGTGTTTAAGAATCGGG
CGTACTGCACCCAGAGCGAGAGACACTGGGTGCCGGAGCAGAGCCGGTGCCTGCCCCAGCAGATTATCTT
CTACATTGGACACGGTGAGCTGGGCCACCCTGCCATTCTGGTCAGCCGGAACCTGTTCAAACCCTCACTA
CCCACTCAGAAGTGGAAGGAGGTGCAGGACCAGCCTGGGCTCCGCCTGTTCGGCCGTCCTCTGTCTGATT
ACTACGCCTACAGGCCTGTGCGGGAGCAAGACGCCTACTCCACCCTCCTGTCGCACATCTTCCACTGCCG
AAAGGAGTCCTTCATCACCACCGAGAACCTCTTGGCCTCCTGGGTCTTCTGGACCCCGTTACTGGACAGC
CTGGCCTTTGAAGTTCCACGCCCGTACCCAGGGGGAGCCGAGAATGGCCAGCTGCTGGACTTCGAGTTTA
GCGGTGGTCAGCTGACCTTCTCCCAGCAGCAGCTGGAGGTGCTGATACCAGATCTAGGCTCAGTCCCAAA
GCCCAGTGACTTCCAGGTCCTCGGGGCCCGGTATAGACAGAGCCCACTGATTACCGCCTGGCCAGAGGAG
CTGATCTCTAAGCTGGCCAGTGACATTGAAGCCGCCGCGGTGCAGGCGGTACGGCACTTCGGCAAGTTCC
ACCTGGCACTGTCAGGTGGTTCGAGCCCCATAGCTCTCTTCCAGCAGCTGGCCACAGGGCATTACAGCTT
CCCCTGGGCCCACACACACCTCTGGCTGGTTGATGAGCGCTGTGTCCCTCTCTCAGATCCAGACTCCAAC
TTCCAGGGCCTGCAGGCTCACCTGCTACAGCACGTCAGGGTACCCTACTACAACATCCACCCAATGCCAG
TGCACCTGCACCAGCGGCTCTGTGCCGAGGAGGACCAGGGAGCTCAGACCTATGCCAGTGAGATCTCAGC
CCTGGTGGCCAACAGCAGCTTCGACCTGGTGCTCCTGGGCATGGGCACCGACGGGCACACGGCTTCTCTG
TTCCCACAGTCCCCCACAGGCCTGGATGGTGATCAGCTGGTGGTGCTGACGGAGAGCCCGTTCAGGCCAC
ACCAACGCATGAGCCTCAGCCTGCCCCTCATCAACCGAGCCAAGAAGGTAGCTGTCCTGGTCATGGGCAG
GACAAAACGTGAGATCACAACGTTGGTGAGCCGCGTGGGCCATGAACCCAAGAAGTGGCCCATCTCAGGT
GTGGTGCCTCTTTCTGGGCAGCTGGTTTGGTACATGGATTACGAGGCCTTTCTGGGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210665 representing BC042677
Red=Cloning site Green=Tags(s)

MLLAAMCLALLGCLQAQELKGHVSIILLGATGDLAKKYLWQGLFQLYLDEAGKGHSFSFHGAALTAPQQG
QKLMDKALESLSCPKDLVPSRCDELKGQFLQLSQYHQLKTVEDYQTLNKDIETQVQQDGLWEAGRIFYFS
VPPFAYADIARNINSSCRPHPGAWLRVVFEKPFGHDHLSAQQLASELGSFFQEEEMYRVDHYLGKQAVAQ
ILPFRDQNRKALDGLWNRHHVERVEIILKETIDAEGRASFYEEYGVIRDTLQNHLTEILTLVAMELPLNI
SSSATVLQHKLWAFQALRGLQKSSAILGQYQAYSGQVRRELQKPDGFQSLTPTFAGVLVHIDNLRWEGVP
FILMSGKALDERVGYVRIVFKNRAYCTQSERHWVPEQSRCLPQQIIFYIGHGELGHPAILVSRNLFKPSL
PTQKWKEVQDQPGLRLFGRPLSDYYAYRPVREQDAYSTLLSHIFHCRKESFITTENLLASWVFWTPLLDS
LAFEVPRPYPGGAENGQLLDFEFSGGQLTFSQQQLEVLIPDLGSVPKPSDFQVLGARYRQSPLITAWPEE
LISKLASDIEAAAVQAVRHFGKFHLALSGGSSPIALFQQLATGHYSFPWAHTHLWLVDERCVPLSDPDSN
FQGLQAHLLQHVRVPYYNIHPMPVHLHQRLCAEEDQGAQTYASEISALVANSSFDLVLLGMGTDGHTASL
FPQSPTGLDGDQLVVLTESPFRPHQRMSLSLPLINRAKKVAVLVMGRTKREITTLVSRVGHEPKKWPISG
VVPLSGQLVWYMDYEAFLG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC042677
ORF Size 2369 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC042677, AAH42677
RefSeq Size 4583 bp
RefSeq ORF 2369 bp
Locus ID 100198
Gene Summary Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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