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Cul5 (NM_027807) Mouse Tagged ORF Clone
CAT＃: MG210611
- TrueORF®
Cul5 (tGFP-tagged) - Mouse cullin 5 (Cul5)
ORF Plasmid: DDK
"NM_027807" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4921514I20Rik; 8430423K24Rik; AI852817; C030032G03Rik; C330021I08Rik; VACM-1; VACM1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210611 representing NM_027807
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTCTCTCGCGTGGGCAGGCCGGGGTGACCATGTAGCTGGAAAGCCCGAGGAAGCACGGCTGCCCG GGACGAGCTCGGCGCTGACGGCACGCCGCTCCGGCGTCCCCCGCATCCCCCGCCGCGGCCTGCGGGGTCC TGCTGGGAACCCCGGCCTCTCGAGGAGGCCTGGCCCCGAGCGCCGCCAAGTCTCGCCCCGTCTCGCGAGA GTCCAAGTTGAGAACATGGCGACGTCTAATCTGTTAAAGAATAAAGGTTCTCTCCAGTTTGAAGACAAGT GGGACTTCATGCATCCAATTGTTTTGAAGCTTTTACGCCAGGAATCTGTAACAAAACAGCAGTGGTTTGA TCTATTTTCGGATGTACATGCTGTCTGTCTCTGGGATGATAAAGGCTCATCAAAAATTCATCAGGCTTTA AAAGAAGATATTCTTGAGTTTATTAAGCAAGCACAGGCTCGTGTACTGAGCCATCAAGATGACACAGCTT TGCTGAAGGCATATATTGTTGAATGGCGGAAATTCTTCACACAGTGTGATATTTTACCAAAACCTTTTTG TCAATTAGAGGTGACTCTATTGGGTAAACAAAGCAGCAATAAAAAATCAAATATGGAAGACAGTATTGTT CGAAAGCTCATGCTTGATACGTGGAATGAGTCGATTTTTTCAAATATAAAGAACAGACTCCAGGACAGTG CAATGAAGCTGGTGCATGCTGAGAGATTAGGGGAAGCTTTTGATTCCCAGCTGGTCATCGGGGTGCGAGA GTCCTATGTTAATCTTTGCTCCAACCCCGAGGACAAGCTTCAGATCTATAGGGATAATTTTGAGAAGGCA TACTTGGACTCAACAGAGAGGTTTTATAGAACACAGGCACCCTCATATTTACAGCAAAATGGTGTGCAGA ATTACATGAAATATGCAGATGCTAAATTAAAAGAAGAAGAAAAAAGAGCACTCCGATACTTAGAAACACG ACGAGAGTGTAACTCTGTGGAAGCACTCATGGAATGCTGTGTAAATGCGCTGGTGACCTCCTTTAAAGAG ACTATTTTAGCAGAATGCCAAGGCATGATCAAGCGAAATGAAACTGAAAAGTTACATTTGATGTTTTCCT TGATGGACAAAGTTCCTAATGGGATAGAGCCGATGTTGAAGGACTTGGAGGAGCATATTATAAGTGCGGG CCTAGCAGACATGGTGGCCGCAGCTGAAACCATCACTACTGACTCTGAGAAGTATGTGGAGCAATTACTT ACACTGTTTAATAGATTCAGTAAACTGGTCAAAGAAGCTTTTCAGGATGATCCTCGTTTCCTTACTGCAA GAGATAAGGCATATAAAGCAGTTGTTAATGATGCTACTATATTTAAACTTGAATTGCCTTTGAAGCAAAA AGGAGTGGGGTTGAAAACTCAGCCTGAGTCAAAATGCCCCGAGTTGCTTGCCAATTACTGTGACATGTTG TTAAGGAAAACGCCATTAAGCAAAAAACTAACATCTGAGGAGATTGAAGCAAAGCTTAAAGAAGTGCTCT TGGTACTTAAATATGTACAAAACAAAGATGTTTTTATGAGGTATCACAAAGCTCATCTTACCCGACGTCT CATATTGGACATCTCTGCTGATAGTGAGATTGAAGAAAACATGGTAGAGTGGCTAAGAGAAGTTGGTATG CCAGCAGATTATGTGAACAAGCTTGCTAGAATGTTTCAGGACATAAAAGTATCTGAAGACTTGAACCAAG CTTTTAAGGAAATGCACAAAAATAATAAGTTGGCATTACCAGCTGATTCCGTTAATATAAAGATTTTGAA TGCTGGTGCTTGGTCTAGAAGCTCCGAGAAAGTCTTTGTCTCACTTCCTACTGAACTGGAGGATTTGATA CCTGAAGTAGAAGAATTTTACAAAAAAAATCACAGTGGTAGAAAATTACACTGGCACCATCTCATGTCAA ATGGAATTATAACATTTAAAAATGAAGTAGGTCAGTATGATTTGGAAGTAACCACGTTTCAGTTGGCTGT GTTGTTTGCATGGAACCAAAGGCCTAGAGAGAAAATCAGCTTTGAAAATCTAAAACTTGCAACGGAACTC CCAGATGCTGAACTTAGAAGGACTTTATGGTCTTTAGTAGCTTTTCCCAAGCTCAAACGGCAAGTTTTGT TGTATGACCCTCAAGTCAACTCACCCAAAGATTTTACAGAAGGCACCCTCTTCTCAGTGAACCAGGACTT CAGTCTCATAAAAAATGCAAAAGTACAGAAAAGGGGGAAAATCAATTTGATTGGACGCTTGCAGCTCACT ACAGAACGAATGAGAGAAGAAGAAAATGAAGGGATAGTCCAACTAAGAATATTAAGAACCCAGGAAGCCA TCATACAAATAATGAAAATGAGAAAGAAAATTAGCAATGCCCAGCTGCAGACTGAATTAGTAGAAATTCT GAAAAACATGTTCCTGCCTCAGAAGAAGATGATAAAGGAGCAGATGGAGTGGCTGATTGAACACAGGTAC ATCCGGAGGGACGAGGCCGACATCAACACCTTCATCTACATGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210611 representing NM_027807
Red=Cloning site Green=Tags(s) MRSLAWAGRGDHVAGKPEEARLPGTSSALTARRSGVPRIPRRGLRGPAGNPGLSRRPGPERRQVSPRLAR VQVENMATSNLLKNKGSLQFEDKWDFMHPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGSSKIHQAL KEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEVTLLGKQSSNKKSNMEDSIV RKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKA YLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKE TILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLL TLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDML LRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGM PADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLI PEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATEL PDAELRRTLWSLVAFPKLKRQVLLYDPQVNSPKDFTEGTLFSVNQDFSLIKNAKVQKRGKINLIGRLQLT TERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQMEWLIEHRY IRRDEADINTFIYMA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027807 |
| ORF Size | 2565 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027807.3, NP_082083.2 |
| RefSeq Size | 6023 bp |
| RefSeq ORF | 2568 bp |
| Locus ID | 75717 |
| UniProt ID | Q9D5V5 |
| Gene Summary | Core component of multiple SCF-like ECS (Elongin BC-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222170 | Cul5 (untagged) - Mouse cullin 5 (Cul5), transcript variant 1, (10ug) |
CNY 10,930.00 |
|
| MR210611 | Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 1 |
CNY 6,264.00 |
|
| MR210611L3 | Lenti ORF clone of Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 1 |
CNY 9,030.00 |
|
| MR210611L4 | Lenti ORF clone of Cul5 (mGFP-tagged) - Mouse cullin 5 (Cul5), transcript variant 1 |
CNY 9,030.00 |
