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Cul5 (NM_027807) Mouse Untagged Clone
CAT＃: MC222170
Cul5 (untagged) - Mouse cullin 5 (Cul5), transcript variant 1, (10ug)
CNY 10,930.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4921514I20Rik; 8430423K24Rik; AI852817; C030032G03Rik; C330021I08Rik; VACM-1; VACM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222170 representing NM_027807
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTCTCTCGCGTGGGCAGGCCGGGGTGACCATGTAGCTGGAAAGCCCGAGGAAGCACGGCTGCCCG GGACGAGCTCGGCGCTGACGGCACGCCGCTCCGGCGTCCCCCGCATCCCCCGCCGCGGCCTGCGGGGTCC TGCTGGGAACCCCGGCCTCTCGAGGAGGCCTGGCCCCGAGCGCCGCCAAGTCTCGCCCCGTCTCGCGAGA GTCCAAGTTGAGAACATGGCGACGTCTAATCTGTTAAAGAATAAAGGTTCTCTCCAGTTTGAAGACAAGT GGGACTTCATGCATCCAATTGTTTTGAAGCTTTTACGCCAGGAATCTGTAACAAAACAGCAGTGGTTTGA TCTATTTTCGGATGTACATGCTGTCTGTCTCTGGGATGATAAAGGCTCATCAAAAATTCATCAGGCTTTA AAAGAAGATATTCTTGAGTTTATTAAGCAAGCACAGGCTCGTGTACTGAGCCATCAAGATGACACAGCTT TGCTGAAGGCATATATTGTTGAATGGCGGAAATTCTTCACACAGTGTGATATTTTACCAAAACCTTTTTG TCAATTAGAGGTGACTCTATTGGGTAAACAAAGCAGCAATAAAAAATCAAATATGGAAGACAGTATTGTT CGAAAGCTCATGCTTGATACGTGGAATGAGTCGATTTTTTCAAATATAAAGAACAGACTCCAGGACAGTG CAATGAAGCTGGTGCATGCTGAGAGATTAGGGGAAGCTTTTGATTCCCAGCTGGTCATCGGGGTGCGAGA GTCCTATGTTAATCTTTGCTCCAACCCCGAGGACAAGCTTCAGATCTATAGGGATAATTTTGAGAAGGCA TACTTGGACTCAACAGAGAGGTTTTATAGAACACAGGCACCCTCATATTTACAGCAAAATGGTGTGCAGA ATTACATGAAATATGCAGATGCTAAATTAAAAGAAGAAGAAAAAAGAGCACTCCGATACTTAGAAACACG ACGAGAGTGTAACTCTGTGGAAGCACTCATGGAATGCTGTGTAAATGCGCTGGTGACCTCCTTTAAAGAG ACTATTTTAGCAGAATGCCAAGGCATGATCAAGCGAAATGAAACTGAAAAGTTACATTTGATGTTTTCCT TGATGGACAAAGTTCCTAATGGGATAGAGCCGATGTTGAAGGACTTGGAGGAGCATATTATAAGTGCGGG CCTAGCAGACATGGTGGCCGCAGCTGAAACCATCACTACTGACTCTGAGAAGTATGTGGAGCAATTACTT ACACTGTTTAATAGATTCAGTAAACTGGTCAAAGAAGCTTTTCAGGATGATCCTCGTTTCCTTACTGCAA GAGATAAGGCATATAAAGCAGTTGTTAATGATGCTACTATATTTAAACTTGAATTGCCTTTGAAGCAAAA AGGAGTGGGGTTGAAAACTCAGCCTGAGTCAAAATGCCCCGAGTTGCTTGCCAATTACTGTGACATGTTG TTAAGGAAAACGCCATTAAGCAAAAAACTAACATCTGAGGAGATTGAAGCAAAGCTTAAAGAAGTGCTCT TGGTACTTAAATATGTACAAAACAAAGATGTTTTTATGAGGTATCACAAAGCTCATCTTACCCGACGTCT CATATTGGACATCTCTGCTGATAGTGAGATTGAAGAAAACATGGTAGAGTGGCTAAGAGAAGTTGGTATG CCAGCAGATTATGTGAACAAGCTTGCTAGAATGTTTCAGGACATAAAAGTATCTGAAGACTTGAACCAAG CTTTTAAGGAAATGCACAAAAATAATAAGTTGGCATTACCAGCTGATTCCGTTAATATAAAGATTTTGAA TGCTGGTGCTTGGTCTAGAAGCTCCGAGAAAGTCTTTGTCTCACTTCCTACTGAACTGGAGGATTTGATA CCTGAAGTAGAAGAATTTTACAAAAAAAATCACAGTGGTAGAAAATTACACTGGCACCATCTCATGTCAA ATGGAATTATAACATTTAAAAATGAAGTAGGTCAGTATGATTTGGAAGTAACCACGTTTCAGTTGGCTGT GTTGTTTGCATGGAACCAAAGGCCTAGAGAGAAAATCAGCTTTGAAAATCTAAAACTTGCAACGGAACTC CCAGATGCTGAACTTAGAAGGACTTTATGGTCTTTAGTAGCTTTTCCCAAGCTCAAACGGCAAGTTTTGT TGTATGACCCTCAAGTCAACTCACCCAAAGATTTTACAGAAGGCACCCTCTTCTCAGTGAACCAGGACTT CAGTCTCATAAAAAATGCAAAAGTACAGAAAAGGGGGAAAATCAATTTGATTGGACGCTTGCAGCTCACT ACAGAACGAATGAGAGAAGAAGAAAATGAAGGGATAGTCCAACTAAGAATATTAAGAACCCAGGAAGCCA TCATACAAATAATGAAAATGAGAAAGAAAATTAGCAATGCCCAGCTGCAGACTGAATTAGTAGAAATTCT GAAAAACATGTTCCTGCCTCAGAAGAAGATGATAAAGGAGCAGATGGAGTGGCTGATTGAACACAGGTAC ATCCGGAGGGACGAGGCCGACATCAACACCTTCATCTACATGGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_027807 |
| Insert Size | 2568 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_027807.3, NP_082083.2 |
| RefSeq Size | 6023 bp |
| RefSeq ORF | 2568 bp |
| Locus ID | 75717 |
| UniProt ID | Q9D5V5 |
| Gene Summary | Core component of multiple SCF-like ECS (Elongin BC-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210611 | Cul5 (tGFP-tagged) - Mouse cullin 5 (Cul5) |
CNY 7,890.00 |
|
| MR210611 | Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 1 |
CNY 6,264.00 |
|
| MR210611L3 | Lenti ORF clone of Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 1 |
CNY 9,030.00 |
|
| MR210611L4 | Lenti ORF clone of Cul5 (mGFP-tagged) - Mouse cullin 5 (Cul5), transcript variant 1 |
CNY 9,030.00 |
