Hcn3 (NM_008227) Mouse Tagged ORF Clone
CAT#: MG210605
- TrueORF®
Hcn3 (tGFP-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3)
ORF Plasmid: DDK
"NM_008227" in other vectors (4)
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CNY 7,304.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BCNG-4; Bcng4; Hac3; Hcn4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210605 representing NM_008227
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGGAGGCGCGGCCGGCGGCGGGGGCCGGCGAAGCGGCGACCCCTGCACGCGAGACGCCTCCTG CGGCTCCGGCCCAGGCCCGCGCGGCCTCAGGTGGGGTGCCGGAGTCTGCGCCCGAGCCGAAGAGGCGGCA GCTCGGGACGCTGCTGCAGCCGACGGTCAACAAGTTCTCTCTCCGGGTCTTCGGCAGCCACAAAGCAGTA GAAATCGAGCAGGAGAGGGTGAAGTCCGCCGGGGCCTGGATCATCCACCCCTACAGCGACTTCCGGTTTT ACTGGGATCTCATCATGCTGCTGCTGATGGTGGGGAACCTCATAGTTCTGCCTGTGGGTATCACTTTCTT CAAGGAGGAGAACTCTCCACCCTGGATCGTCTTCAATGTCCTCTCTGACACTTTCTTCCTGCTGGATCTG GTGCTCAACTTCCGAACTGGCATCGTGGTGGAGGAAGGTGCCGAGATCCTGCTGGCGCCAAGGGCCATCC GAACGCGTTACCTGCGCACCTGGTTCCTGGTTGATCTGATCTCCTCCATCCCTGTGGATTATATCTTCCT AGTGGTGGAGCTGGAGCCACGACTAGATGCTGAGGTCTACAAAACGGCACGGGCCCTGCGCATCGTTAGA TTCACCAAGATCCTTAGCCTGCTGCGGCTGCTCCGCCTCTCCCGCCTCATCCGCTACATACACCAGTGGG AGGAGATCTTTCACATGACCTACGACCTGGCCAGTGCAGTGGTTCGCATCTTCAACCTCATTGGAATGAT GTTGCTGCTGTGTCACTGGGACGGCTGTCTGCAGTTTCTGGTCCCTATGCTGCAGGACTTCCCGTCCGAC TGCTGGGTCTCCATGAACCGCATGGTGAACCACTCGTGGGGCCGCCAGTATTCCCACGCCCTGTTCAAGG CCATGAGTCACATGCTATGCATTGGCTATGGGCAGCAGGCACCGGTAGGCATGCCTGACGTCTGGCTCAC CATGCTCAGTATGATTGTGGGCGCCACGTGTTATGCCATGTTCATCGGTCACGCCACCGCCCTCATCCAG TCCCTGGACTCTTCCCGGCGACAGTACCAGGAGAAGTACAAGCAGGTGGAGCAGTACATGTCCTTCCACA AGCTGCCCGCTGACACCCGGCAGCGCATCCACGAGTACTACGAGCATCGCTACCAGGGCAAGATGTTTGA TGAAGAGAGCATCCTGGGGGAGCTGAGCGAGCCACTTCGGGAGGAGATTATTAACTTCACCTGCCGGGGC CTGGTGGCCCACATGCCGCTGTTTGCTCATGCTGACCCCAGCTTCGTCACCGCAGTGCTCACCAAGCTCC GTTTTGAGGTCTTCCAACCAGGGGACCTGGTGGTGCGTGAGGGCTCCGTGGGCAGGAAGATGTACTTCAT CCAGCACGGGCTGCTGAGTGTGCTGGCACGTGGCGCCCGCGACACCCGCCTCACTGATGGATCCTACTTT GGGGAGATCTGCCTGCTGACTCGAGGTCGGAGAACAGCCAGTGTAAGGGCTGACACCTATTGTCGCCTCT ACTCGCTCAGCGTGGACCACTTCAATGCGGTGCTTGAGGAGTTCCCAATGATGCGCAGGGCTTTTGAGAC GGTGGCCATGGACCGGCTTCGGCGCATCGGCAAAAAGAATTCGATACTGCAGCGGAAACGCTCTGAGCCG AGTCCAGGCAGCAGCGGTGGCGTCATGGAGCAGCATTTGGTACAACACGACAGAGACATGGCTCGTGGTG TTCGGGGCCTGGCTCCTGGTACAGGAGCTCGACTCAGTGGAAAGCCAGTGCTGTGGGAACCACTGGTGCA CGCCCCTCTGCAGGCAGCTGCTGTGACCTCCAACGTGGCCATAGCCTTGACTCACCAGCGAGGCCCTCTG CCCCTCTCCCCTGATTCTCCAGCCACCCTCCTAGCTCGATCTGCTAGACGCTCAGCAGGCTCCCCAGCCT CCCCACTGGTGCCTGTCCGAGCAGGTCCTCTGCTGGCCCGGGGACCCTGGGCGTCCACTTCTCGCCTGCC TGCTCCACCTGCCCGAACCCTCCATGCCAGCCTATCCCGGACAGGGCGTTCCCAGGTATCTCTGTTGGGC CCTCCCCCAGGAGGAGGTGCTCGGAGGCTAGGACCTCGGGGCCGCCCACTTTCTGCCTCGCAACCCTCTC TGCCTCAGCGAGCAACAGGGGATGGCTCTCCTAGGCGTAAAGGCTCTGGAAGTGAGCGCCTGCCCCCCTC TGGGCTCTTGGCCAAACCTCCAGGGACAGTCCAGCCACCCAGGTCATCAGTGCCTGAGCCAGTTACCCCC AGAGGTCCCCAAATTTCTGCCAACATG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210605 representing NM_008227
Red=Cloning site Green=Tags(s) MEEEARPAAGAGEAATPARETPPAAPAQARAASGGVPESAPEPKRRQLGTLLQPTVNKFSLRVFGSHKAV EIEQERVKSAGAWIIHPYSDFRFYWDLIMLLLMVGNLIVLPVGITFFKEENSPPWIVFNVLSDTFFLLDL VLNFRTGIVVEEGAEILLAPRAIRTRYLRTWFLVDLISSIPVDYIFLVVELEPRLDAEVYKTARALRIVR FTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPMLQDFPSD CWVSMNRMVNHSWGRQYSHALFKAMSHMLCIGYGQQAPVGMPDVWLTMLSMIVGATCYAMFIGHATALIQ SLDSSRRQYQEKYKQVEQYMSFHKLPADTRQRIHEYYEHRYQGKMFDEESILGELSEPLREEIINFTCRG LVAHMPLFAHADPSFVTAVLTKLRFEVFQPGDLVVREGSVGRKMYFIQHGLLSVLARGARDTRLTDGSYF GEICLLTRGRRTASVRADTYCRLYSLSVDHFNAVLEEFPMMRRAFETVAMDRLRRIGKKNSILQRKRSEP SPGSSGGVMEQHLVQHDRDMARGVRGLAPGTGARLSGKPVLWEPLVHAPLQAAAVTSNVAIALTHQRGPL PLSPDSPATLLARSARRSAGSPASPLVPVRAGPLLARGPWASTSRLPAPPARTLHASLSRTGRSQVSLLG PPPGGGARRLGPRGRPLSASQPSLPQRATGDGSPRRKGSGSERLPPSGLLAKPPGTVQPPRSSVPEPVTP RGPQISANM SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008227 |
ORF Size | 2337 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008227.1, NP_032253.1 |
RefSeq Size | 3268 bp |
RefSeq ORF | 2340 bp |
Locus ID | 15168 |
UniProt ID | O88705 |
Gene Summary | Hyperpolarization-activated potassium channel. May also facilitate the permeation of sodium ions.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205963 | Hcn3 (untagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3), (10ug) |
CNY 5,712.00 |
|
MR210605 | Hcn3 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3) |
CNY 5,704.00 |
|
MR210605L3 | Lenti ORF clone of Hcn3 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3) |
CNY 7,700.00 |
|
MR210605L4 | Lenti ORF clone of Hcn3 (mGFP-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3) |
CNY 7,700.00 |