Hcn3 (NM_008227) Mouse Tagged ORF Clone

CAT＃: MG210605

TrueORF^®

Hcn3 (tGFP-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_008227" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,304.00

货期*

现货

规格

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA311496

Goat Anti-HCN3 (aa 715-728) Antibody

CNY 5,371.00

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	BCNG-4; Bcng4; Hac3; Hcn4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210605 representing NM_008227 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGGAGGCGCGGCCGGCGGCGGGGGCCGGCGAAGCGGCGACCCCTGCACGCGAGACGCCTCCTG CGGCTCCGGCCCAGGCCCGCGCGGCCTCAGGTGGGGTGCCGGAGTCTGCGCCCGAGCCGAAGAGGCGGCA GCTCGGGACGCTGCTGCAGCCGACGGTCAACAAGTTCTCTCTCCGGGTCTTCGGCAGCCACAAAGCAGTA GAAATCGAGCAGGAGAGGGTGAAGTCCGCCGGGGCCTGGATCATCCACCCCTACAGCGACTTCCGGTTTT ACTGGGATCTCATCATGCTGCTGCTGATGGTGGGGAACCTCATAGTTCTGCCTGTGGGTATCACTTTCTT CAAGGAGGAGAACTCTCCACCCTGGATCGTCTTCAATGTCCTCTCTGACACTTTCTTCCTGCTGGATCTG GTGCTCAACTTCCGAACTGGCATCGTGGTGGAGGAAGGTGCCGAGATCCTGCTGGCGCCAAGGGCCATCC GAACGCGTTACCTGCGCACCTGGTTCCTGGTTGATCTGATCTCCTCCATCCCTGTGGATTATATCTTCCT AGTGGTGGAGCTGGAGCCACGACTAGATGCTGAGGTCTACAAAACGGCACGGGCCCTGCGCATCGTTAGA TTCACCAAGATCCTTAGCCTGCTGCGGCTGCTCCGCCTCTCCCGCCTCATCCGCTACATACACCAGTGGG AGGAGATCTTTCACATGACCTACGACCTGGCCAGTGCAGTGGTTCGCATCTTCAACCTCATTGGAATGAT GTTGCTGCTGTGTCACTGGGACGGCTGTCTGCAGTTTCTGGTCCCTATGCTGCAGGACTTCCCGTCCGAC TGCTGGGTCTCCATGAACCGCATGGTGAACCACTCGTGGGGCCGCCAGTATTCCCACGCCCTGTTCAAGG CCATGAGTCACATGCTATGCATTGGCTATGGGCAGCAGGCACCGGTAGGCATGCCTGACGTCTGGCTCAC CATGCTCAGTATGATTGTGGGCGCCACGTGTTATGCCATGTTCATCGGTCACGCCACCGCCCTCATCCAG TCCCTGGACTCTTCCCGGCGACAGTACCAGGAGAAGTACAAGCAGGTGGAGCAGTACATGTCCTTCCACA AGCTGCCCGCTGACACCCGGCAGCGCATCCACGAGTACTACGAGCATCGCTACCAGGGCAAGATGTTTGA TGAAGAGAGCATCCTGGGGGAGCTGAGCGAGCCACTTCGGGAGGAGATTATTAACTTCACCTGCCGGGGC CTGGTGGCCCACATGCCGCTGTTTGCTCATGCTGACCCCAGCTTCGTCACCGCAGTGCTCACCAAGCTCC GTTTTGAGGTCTTCCAACCAGGGGACCTGGTGGTGCGTGAGGGCTCCGTGGGCAGGAAGATGTACTTCAT CCAGCACGGGCTGCTGAGTGTGCTGGCACGTGGCGCCCGCGACACCCGCCTCACTGATGGATCCTACTTT GGGGAGATCTGCCTGCTGACTCGAGGTCGGAGAACAGCCAGTGTAAGGGCTGACACCTATTGTCGCCTCT ACTCGCTCAGCGTGGACCACTTCAATGCGGTGCTTGAGGAGTTCCCAATGATGCGCAGGGCTTTTGAGAC GGTGGCCATGGACCGGCTTCGGCGCATCGGCAAAAAGAATTCGATACTGCAGCGGAAACGCTCTGAGCCG AGTCCAGGCAGCAGCGGTGGCGTCATGGAGCAGCATTTGGTACAACACGACAGAGACATGGCTCGTGGTG TTCGGGGCCTGGCTCCTGGTACAGGAGCTCGACTCAGTGGAAAGCCAGTGCTGTGGGAACCACTGGTGCA CGCCCCTCTGCAGGCAGCTGCTGTGACCTCCAACGTGGCCATAGCCTTGACTCACCAGCGAGGCCCTCTG CCCCTCTCCCCTGATTCTCCAGCCACCCTCCTAGCTCGATCTGCTAGACGCTCAGCAGGCTCCCCAGCCT CCCCACTGGTGCCTGTCCGAGCAGGTCCTCTGCTGGCCCGGGGACCCTGGGCGTCCACTTCTCGCCTGCC TGCTCCACCTGCCCGAACCCTCCATGCCAGCCTATCCCGGACAGGGCGTTCCCAGGTATCTCTGTTGGGC CCTCCCCCAGGAGGAGGTGCTCGGAGGCTAGGACCTCGGGGCCGCCCACTTTCTGCCTCGCAACCCTCTC TGCCTCAGCGAGCAACAGGGGATGGCTCTCCTAGGCGTAAAGGCTCTGGAAGTGAGCGCCTGCCCCCCTC TGGGCTCTTGGCCAAACCTCCAGGGACAGTCCAGCCACCCAGGTCATCAGTGCCTGAGCCAGTTACCCCC AGAGGTCCCCAAATTTCTGCCAACATG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210605 representing NM_008227 Red=Cloning site Green=Tags(s) MEEEARPAAGAGEAATPARETPPAAPAQARAASGGVPESAPEPKRRQLGTLLQPTVNKFSLRVFGSHKAV EIEQERVKSAGAWIIHPYSDFRFYWDLIMLLLMVGNLIVLPVGITFFKEENSPPWIVFNVLSDTFFLLDL VLNFRTGIVVEEGAEILLAPRAIRTRYLRTWFLVDLISSIPVDYIFLVVELEPRLDAEVYKTARALRIVR FTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPMLQDFPSD CWVSMNRMVNHSWGRQYSHALFKAMSHMLCIGYGQQAPVGMPDVWLTMLSMIVGATCYAMFIGHATALIQ SLDSSRRQYQEKYKQVEQYMSFHKLPADTRQRIHEYYEHRYQGKMFDEESILGELSEPLREEIINFTCRG LVAHMPLFAHADPSFVTAVLTKLRFEVFQPGDLVVREGSVGRKMYFIQHGLLSVLARGARDTRLTDGSYF GEICLLTRGRRTASVRADTYCRLYSLSVDHFNAVLEEFPMMRRAFETVAMDRLRRIGKKNSILQRKRSEP SPGSSGGVMEQHLVQHDRDMARGVRGLAPGTGARLSGKPVLWEPLVHAPLQAAAVTSNVAIALTHQRGPL PLSPDSPATLLARSARRSAGSPASPLVPVRAGPLLARGPWASTSRLPAPPARTLHASLSRTGRSQVSLLG PPPGGGARRLGPRGRPLSASQPSLPQRATGDGSPRRKGSGSERLPPSGLLAKPPGTVQPPRSSVPEPVTP RGPQISANM SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII Cloning Scheme for this gene Plasmid Map
ACCN	NM_008227
ORF Size	2337 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_008227.1, NP_032253.1
RefSeq Size	3268 bp
RefSeq ORF	2340 bp
Locus ID	15168
UniProt ID	O88705
Gene Summary	Hyperpolarization-activated potassium channel. May also facilitate the permeation of sodium ions.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC205963	Hcn3 (untagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,712.00
MR210605	Hcn3 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,704.00 CNY 5,800.00
MR210605L3	Lenti ORF clone of Hcn3 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
MR210605L4	Lenti ORF clone of Hcn3 (mGFP-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00

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