Slc39a6 (BC055012) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Ermelin
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210539 representing BC055012 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAGATTTATCTGTAATCATGATCTTGACCTTTGCCCTTTGGGTTACAAGCCCCCTTCATGAAC TACAATCAACAGCTGCTTTCTCTCAGACTACTGAGAAAATAAATTCAAATTGGGAACCTGGTGTTAATGT TGACTTGGCAGTTACCATGCAGCGACACCATCTGCAGCAGCTATTCTACCGCTACGGAGAGAATGATTCC TTGTCTGTTGAAGGCTTCAGAAAATTGCTTCAGAACATAGGCATAGATAAGATTAAAAGAGTCCATATAC ACCATGACCACGAGCATCATGCTGACCACGAGCATCACTCGGACCATGAGCATCACTCGGACCACGAGCA TCACTCGGACCACGAGCATCACTCGGACCACGAGCATCACTCGGACCACGAGCACCATTCCCACCGCAGT CACACGGTTGCTGGTAAAAACAATCGGAAAGCCTTTTGTCCAGACCTTGACTCTGATAATTCAGGTAAAA ATCCTAGAACTAGTCTAGGGAAAGGATCTCGCCCAGCAGAGCACATGAATGGTAGGAGGAACATCAAGGA GAGTGCAAGCTCTAGTGAAGTGACCTCGGCGGTATACAACGTTGTCTCTGAAGGAACTCGCTTTGTAGAG ACAATAGAGACTCCAAAACCTGGGAGACGCACCAAAGATGTAAACCCTTCTACCCCACCCAGCATCACGG AGAAAAGCCGAGTGGGCCGGCTGAGTCGGCTAGCTAGGAAGAAAAGCAATGAGTCTGTGAGTGAGCCCAG AAAGAGCTTTATGTATTCCAGAAACACAAATGACAATATTCAGGAGTGTTTCAATACAACCAAGCTGCTG ACATCCCATGGCATGAGCATCCAGGCTCTGTTGAATGCAACGGAATTTAACTATCTCTGCCCAGCCATCA TCAATCAAATTGATGCTCGGGCTTGTCTGATTCATACAGCAAGTGAGAAGAAGGCAGAAATCCCTCCAAA GACCTATTCTTTACAAATAGCCTGGCTTGGTGGCTTCATAGCCATTTCCATCATCAGTTTCCTGTCTCTG CTGGGAGTCATCTTGGTGCCACTCATGAACCGGGTATTTTTCAAGTTCCTGCTGAGCTTCCTCGTGGCGC TGGCCGTCGGAACGCTGAGTGGCGATGCTCTGTTACATCTTCTCCCACACTCTCATGCAAGTCATCAGCA CAGTCATAGCCATGAAGAGCCAGCGATGGAAATGAAAAGAGGCCCCCTGTTCAGCCACCTGTCGGCTCAG AATATAGAAGAAAGCTCCTATTTTGATTCCACGTGGAAAGGTCTGACGGCTCTAGGGGGCTTATATTTCA TGTTTCTTGTGGAACACGTACTCACACTGATCAAGCAATTTAAAGATAAGAAAAAGAAGAATCAAAAGAA ACCTGAAAATGATGAGGATGTGGAGAGCAAGAAGCAGCTGTCCAAATACGACTCTCAGCTTTCCTCAAAT GAAGAGAAGGTGGACCCAGGGGAACGACCTGAAAGCTATCTGCGAGCCGACTCCCAAGAGCCCTCCCCCT TTGATTCCCAGCAGCCGACGATGTTGGAAGAGGAAGAGGTCATGATAGCCCATGCACACCCACAAGAAGT CTACAATGAATATGTGCCCAGGGGCTGCAAGAACAAGTGCCATTCACACTTCCACGATACGCTGGGCCAG TCCGACGACCTCATCCACCACCATCACGACTACCATCACATTCTGCACCACCACCACCACCAGAACCACC ACCCTCACAGCCACAGCCAGCGCTACTCTCGAGAGGAGCTGAAGGACGCCGGCATTGCCACATTGGCCTG GATGGTGATCATGGGCGACGGGCTGCACAATTTCAGTGACGGCCTTGCTATTGGTGCTGCCTTCACCGAG GGTTTGTCCAGTGGGCTAAGCACCTCTGTCGCTGTGTTCTGTCATGAACTGCCTCATGAACTAGGTGACT TTGCTGTTTTGCTAAAGGCTGGCATGACTGTCAAGCAGGCTGTGCTCTATAATGCTCTGTCAGCCATGTT GGCCTACCTTGGAATGGCAACAGGGATATTCATCGGGCATTATGCAGAAAATGTTTCTATGTGGATATTC GCACTCACTGCCGGCTTGTTCATGTATGTCGCTCTGGTTGACATGGTGCCTGAGATGTTGCACAATGATG CTAGTGATCACGGATGCAGCCGTTGGGGATATTTCTTCCTGCAGAATGCTGGGATACTTCTCGGTTTTGG AATTATGTTACTCATTTCCATATTTGAGCATAAAATTGTGTTTCGTATAAATTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210539 representing BC055012 Red=Cloning site Green=Tags(s) MATDLSVIMILTFALWVTSPLHELQSTAAFSQTTEKINSNWEPGVNVDLAVTMQRHHLQQLFYRYGENDS LSVEGFRKLLQNIGIDKIKRVHIHHDHEHHADHEHHSDHEHHSDHEHHSDHEHHSDHEHHSDHEHHSHRS HTVAGKNNRKAFCPDLDSDNSGKNPRTSLGKGSRPAEHMNGRRNIKESASSSEVTSAVYNVVSEGTRFVE TIETPKPGRRTKDVNPSTPPSITEKSRVGRLSRLARKKSNESVSEPRKSFMYSRNTNDNIQECFNTTKLL TSHGMSIQALLNATEFNYLCPAIINQIDARACLIHTASEKKAEIPPKTYSLQIAWLGGFIAISIISFLSL LGVILVPLMNRVFFKFLLSFLVALAVGTLSGDALLHLLPHSHASHQHSHSHEEPAMEMKRGPLFSHLSAQ NIEESSYFDSTWKGLTALGGLYFMFLVEHVLTLIKQFKDKKKKNQKKPENDEDVESKKQLSKYDSQLSSN EEKVDPGERPESYLRADSQEPSPFDSQQPTMLEEEEVMIAHAHPQEVYNEYVPRGCKNKCHSHFHDTLGQ SDDLIHHHHDYHHILHHHHHQNHHPHSHSQRYSREELKDAGIATLAWMVIMGDGLHNFSDGLAIGAAFTE GLSSGLSTSVAVFCHELPHELGDFAVLLKAGMTVKQAVLYNALSAMLAYLGMATGIFIGHYAENVSMWIF ALTAGLFMYVALVDMVPEMLHNDASDHGCSRWGYFFLQNAGILLGFGIMLLISIFEHKIVFRINF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC055012
ORF Size	2297 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC055012, AAH55012
RefSeq Size	3610 bp
RefSeq ORF	2297 bp
Locus ID	106957
Gene Summary	May act as a zinc-influx transporter.[UniProtKB/Swiss-Prot Function]

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