Ikbkb (BC037723) Mouse Tagged ORF Clone

CAT#: MG210500

  • TrueORF®

Ikbkb (tGFP-tagged) - Mouse inhibitor of kappaB kinase beta (cDNA clone MGC:47377 IMAGE:4482634)



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Mouse Protein / Lysate Request

CNY 8,270.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms IKK2, IKK-2, IKK[b], IKK-beta
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210500 representing BC037723
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGGTCACCGTCCCTCCCAACCCAGACATGTGGAGCCTGGGAAATGAAAGAACGCCTGGGGACCG
GGGGATTTGGAAACGTCATCCGGTGGCACAATCAGGCGACAGGTGAACAGATCGCCATCAAGCAATGCCG
ACAGGAGCTCAGCCCAAAGAACAGAGACCGCTGGTGCCTCGAAATCCAGATCATGAGAAGGCTGAACCAT
CCCAATGTGGTGGCTGCCCGGGATGTCCCAGAGGGGATGCAGAACCTGGCACCCAATGATTTGCCACTGC
TGGCCATGGAGTACTGCCAAGGAGGAGATCTCCGAAGATACTTGAACCAGTTCGAGAACTGCTGTGGCCT
GCGGGAAGGAGCTGTCCTTACCCTGCTGAGTGACATCGCATCGGCTCTTAGATACCTTCACGAAAACAGA
ATCATCCATCGAGACCTGAAGCCAGAAAACATCGTTCTGCAGCAAGGAGAGAAAAGATTAATACACAAAA
TTATTGATCTAGGATATGCCAAGGAGCTGGATCAGGGCAGTCTGTGCACGTCATTTGTGGGGACTCTGCA
ATACCTGGCGCCAGAGCTTCTGGAGCAGCAGAAGTACACCGTGACCGTTGACTACTGGAGCTTCGGCACC
CTGGCCTTCGAGTGCATCACTGGCTTCCGGCCCTTCCTCCCTAACTGGCAGCCTGTGCAGTGGCACTCCA
AAGTCCGGCAGAAGAGCGAAGTGGACATCGTTGTTAGTGAAGACTTGAATGGAGCAGTGAAGTTTTCAAG
TTCGCTACCCTTCCCCAATAATCTTAACAGTGTCTTGGCTGAACGGCTGGAGAAGTGGCTGCAGCTGATG
CTTATGTGGCACCCTCGGCAAAGGGGCACGGATCCCCAGTATGGCCCCAACGGCTGCTTCAGAGCCCTGG
ATGACATCTTGAACTTGAAGCTGGTTCATGTCTTGAACATGGTCACAGGCACCGTTCACACATACCCCGT
GACGGAGGATGAGAGTCTGCAGAGCTTAAAAACCAGAATCCAGGAAGACACGGGGATCCTGGAGACAGAC
CAGGAGCTGCTGCAAGAGGCAGGGCTGGTGCTGCTCCCTGACAAGCCTGCTACTCAGTGCATCTCAGACA
GCAAGACAAACGAGGGCCTCACGTTGGACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCAACTA
TGAGACTCAGATCACCCCCCGACCCCAACCGGAAAGTGTCAGCTGTATCCTTCAGGAGCCCAAGCGGAAC
CTCTCCTTCTTCCAGCTGAGGAAAGTGTGGGGCCAAGTCTGGCACAGCATCCAGACGCTGAAGGAAGACT
GTAACCGGCTGCAGCAGGGACAGCGAGCAGCCATGATGAGTCTCCTCCGGAATAACAGCTGCCTCTCTAA
GATGAAGAACGCCATGGCCTCCACGGCCCAGCAGCTCAAGGCCAAGCTGGACTTCTTCAAAACCAGCATC
CAGATCGACCTGGAGAAGTATAAAGAGCAGACCGAGTTTGGGATCACCTCAGATAAATTGCTGCTGGCTT
GGCGGGAGATGGAGCAGGCTGTGGAGCAGTGTGGGCGGGAGAATGACGTGAAGCATCTAGTAGAGCGGAT
GATGGCACTGCAGACTGACATTGTGGACCTGCAGAGGAGCCCGATGGGTCGGAAGCAGGGGGGCACCCTG
GATGACCTAGAGGAACAAGCGAGGGAGCTCTACCGAAGACTCAGGGAGAAGCCAAGAGACCAAAGGACAG
AAGGTGACAGCCAGGAGATGGTACGGCTGCTGCTTCAGGCAATCCAAAGCTTTGAGAAGAAAGTTCGGGT
GATTTATACACAGCTCAGTAAGACCGTGGTTTGTAAGCAGAAGGCACTGGAGTTGCTGCCCAAGGTAGAA
GAGGTAGTGAGCCTTATGAACGAGGACGAGAGGACCGTGGTCCGGCTTCAGGAGAAGCGGCAGAAGGAAC
TCTGGAACCTCCTGAAGATCGCCTGTAGCAAAGTCCGAGGTCCCGTGAGTGGAAGCCCAGACAGCATGAA
TGTGTCTCGACTCAGTCACCCTGGTCAGCTAATGTCCCAGCCTTCCAGTGCCTGTGACAGCTTACCTGAA
TCAGACAAGAAAAGTGAAGAACTGGTGGCCGAAGCCCACGCCCTCTGCTCCCGGCTAGAAAGTGCGCTGC
AGGACACTGTGAAGGAGCAAGACAGAAGCTTCACGACTCTAGACTGGAGCTGGTTACAGATGGAGGATGA
AGAAAGGTGTAGCCTGGAGCAGGCCTGTGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210500 representing BC037723
Red=Cloning site Green=Tags(s)

MSWSPSLPTQTCGAWEMKERLGTGGFGNVIRWHNQATGEQIAIKQCRQELSPKNRDRWCLEIQIMRRLNH
PNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRRYLNQFENCCGLREGAVLTLLSDIASALRYLHENR
IIHRDLKPENIVLQQGEKRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGT
LAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGAVKFSSSLPFPNNLNSVLAERLEKWLQLM
LMWHPRQRGTDPQYGPNGCFRALDDILNLKLVHVLNMVTGTVHTYPVTEDESLQSLKTRIQEDTGILETD
QELLQEAGLVLLPDKPATQCISDSKTNEGLTLDMDLVFLFDNSKINYETQITPRPQPESVSCILQEPKRN
LSFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMSLLRNNSCLSKMKNAMASTAQQLKAKLDFFKTSI
QIDLEKYKEQTEFGITSDKLLLAWREMEQAVEQCGRENDVKHLVERMMALQTDIVDLQRSPMGRKQGGTL
DDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVE
EVVSLMNEDERTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNVSRLSHPGQLMSQPSSACDSLPE
SDKKSEELVAEAHALCSRLESALQDTVKEQDRSFTTLDWSWLQMEDEERCSLEQACD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC037723
ORF Size 2273 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC037723, AAH37723
RefSeq Size 3572 bp
RefSeq ORF 2273 bp
Locus ID 16150
Gene Summary Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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