Mapk7 (BC100398) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	BMK1, ERK5, PRKM7, Erk5-T
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210486 representing BC100398 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAACCGCTGAAGGAGGAGGACGGCGAAGATGGCTCTGGGGAGCCCCCTGGGAGGGTGAAGGCAG AACCCGTTCACACCGCTGCCTCTGTGGTGGCCAAGAACCTGGCCCTGCTCAAAGCCCGCTCCTTCGACGT GACCTTTGACGTGGGGGACGAGTACGAGATCATCGAGACCATAGGCAATGGGGCCTACGGGGTGGTGTCT TCGGCGCGCCGCCGCCTCACGGGCCAGCAGGTGGCCATCAAGAAGATACCTAATGCTTTTGATGTGGTGA CCAATGCCAAACGGACCCTCAGGGAGCTGAAGATCCTCAAACACTTCAAACACGACAATATCATCGCCAT CAAGGACATCCTGAAGCCTACTGTGCCCTATGGAGAATTCAGATCTGTCTATGTGGTACTGGACCTCATG GAGAGCGACCTACACCAGATCATTCACTCTTCACAGCCGCTCACCCTGGAACATGTGAGATACTTCCTGT ACCAGCTGCTTCGGGGCCTCAAATACATGCACTCTGCTCAGGTCATCCACCGTGATCTTAAACCCTCTAA CCTTCTGGTCAATGAGAACTGTGAGCTCAAGATCGGTGACTTTGGAATGGCCCGTGGTCTCTGTACTTCC CCTGCCGAGCACCAGTACTTCATGACTGAGTATGTGGCTACTCGCTGGTACCGTGCCCCGGAGCTCATGC TTTCCCTGCACGAGTATACGCAGGCAATCGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGC TCGGCGCCAGCTCTTCCCAGGCAAAAACTACGTGCACCAGTTACAGCTGATCATGATGGTGTTGGGAACT CCGTCACCAGCTGTGATTCAGGCTGTGGGGGCTGAAAGGGTGCGAGCCTATATCCAGAGCCTGCCACCAA GGCAACCTGTGCCTTGGGAGACAGTATACCCAGGTGCTGACCGCCAGGCCCTCTCCCTGCTGGGACGCAT GTTGCGATTTAAACCCAGTGCCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAGTAC CATGACCCTGATGATGAGCCTGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTACCA GGGAGCGCATTAAGGAGGCCATTGTGGCTGAGATTGAGGACTTCCACGCACGACGGGAGGGCATCCGCCA ACAAATCCGCTTCCAGCCTTCTCTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTTGAGATGCCC AGTCCCTGGGCTCCAAGTGGAGACTGTGCCATGGAGTCGCCTCCTCCAGCACTGCCACCATGCTCTGATC CTGCACCTGACACCGTTGATCTGACTCTGCAGCCTGCCCCGCCGGCCAGTGAGCTTGCTCCACCAAAAAG AGAGGGTGCCATCTCCGACAATACCAAAGCAGCCCTCAAAGCTGCCCTGCTCAAGTCCCTAAGGAGCAGG CTCAGAGATGGGCCCAGTGCACCCTTGGAGGCGCCTGAGCCTCGAAAGCCCGTGACAGCTCAGGAACGCC AGCGGGAACGAGAAGAGAAGCGCAGGAGGCGACAAGAGAGAGCCAAGGAGCGGGAGAAGCGACGACAAGA GAGAGAACGCAAGGAGAGGGGGGCTGGTACCTTGGGGGGCCCCTCTACTGACCCTCTGGCTGGACTGGTG CTCAGTGACAATGACCGAAGCCTGCTAGAGCGGTGGACTCGCATGGCTAGGCCTCCTGCCCCTGCCCCTG CCCCAGCGCCAGCACCAGCGCCAGCACCGTCCTCTGCCCAGCCCACTAGTACTCCTACTGGCCCCGTATC TCAGTCTACTGGTCCTCTACAGCCTGCAGGCTCTATTCCGGGTCCTGCCTCCCAGCCTGTTTGCCCACCC CCTGGCCCTGTTCCCCAGCCTGCTGGCCCTATCCCTGCTCCGCTCCAGACTGCCCCTTCCACTAGCCTTT TGGCCTCCCAGTCACTTGTGCCACCTAGTGGGTTGCCTGGTTCTGGTGCCCCAGAAGTTCTGCCTTACTT CCCATCTGGCCCACCACCTCCAGATCCTGGGCTCACCCCTCAGCCTTCTACATCAGAGTCACCTGATGTC AACCTGGTGACTCAGCAGCTGTCCAAGTCTCAGGTGGAGGACCCCCTGCCTCCTGTGTTCTCTGGCACTC CAAAGGGCAGTGGGGCTGGCTACGGAGTTGGCTTTGATCTGGAGGAATTCTTAAATCAATCTTTTGATAT GGGTGTGGCTGATGGGCCACAGGATGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210486 representing BC100398 Red=Cloning site Green=Tags(s) MAEPLKEEDGEDGSGEPPGRVKAEPVHTAASVVAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVS SARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILKPTVPYGEFRSVYVVLDLM ESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTS PAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGT PSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFKPSARISAAAALRHPFLAKY HDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPVCPDVEMP SPWAPSGDCAMESPPPALPPCSDPAPDTVDLTLQPAPPASELAPPKREGAISDNTKAALKAALLKSLRSR LRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGTLGGPSTDPLAGLV LSDNDRSLLERWTRMARPPAPAPAPAPAPAPAPSSAQPTSTPTGPVSQSTGPLQPAGSIPGPASQPVCPP PGPVPQPAGPIPAPLQTAPSTSLLASQSLVPPSGLPGSGAPEVLPYFPSGPPPPDPGLTPQPSTSESPDV NLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC100398
ORF Size	2270 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC100398, AAI00399
RefSeq Size	3077 bp
RefSeq ORF	2270 bp
Locus ID	23939
Gene Summary	Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression (By similarity). Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction (By similarity).[UniProtKB/Swiss-Prot Function]

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