Loxl3 (BC011298) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Lor2, Loxl2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210480 representing BC011298 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGCTGTCAGTGTGTGGTATTGCTGCCCATGGGGACTACTGCTGCTACACTGTCTGTGCAGCTTCA GTGTGGGGTCTCCGTCTCCTTCTATCAGCCCAGAGAAGAAGGTAGGAAGCCAGGGGCTGAGGTTCCGGCT GGCTGGCTTTCCTAGAAAGCCCTATGAAGGTCGAGTGGAGATACAGCGAGCTGGTGAATGGGGCACCATC TGTGATGATGACTTCACGCTGCAGGCTGCCCATGTCCTCTGCCGGGAGCTGGGCTTCACAGAAGCCACAG GGTGGACTCATAGTGCCAAATATGGCCCTGGAACAGGCCGAATCTGGTTGGACAATCTGAGCTGCAGGGG GACTGAAGGAAGTGTGACAGAATGCGCCTCTCGGGGCTGGGGAAACAGTGACTGTACCCATGATGAGGAT GCCGGGGTCATCTGCAAGGACCAGCGCCTTCCCGGTTTCTCAGACTCCAACGTCATTGAGGTGGAGCATC AGTTGCAAGTGGAGGAGGTGCGACTCCGACCTGCGGTGGAGTGGGGAAGGAGACCCCTGCCTGTGACTGA GGGCCTGGTTGAAGTCAGGCTTCCTGAAGGCTGGTCGCAAGTGTGTGACAAAGGCTGGAGTGCCCACAAC AGCCACGTAGTCTGCGGTATGCTGGGCTTCCCTGGAGAAAAGAGAGTCAACATGGCCTTCTACAGGATGC TGGCCCAGAAGAAGCAACACTCCTTTGGTCTGCACAGTGTGGCATGTGTGGGCACAGAAGCCCACCTCTC CCTTTGCTCTCTAGAGTTCTATCGTGCCAATGACACCACCAGGTGCTCTGGGGGAAACCCTGCAGTGGTG AGCTGTGTGCTGGGCCCTCTTTATGCCACCTTTACTGGTCAGAAGAAGCAGCAACACTCTAAGCCTCAAG GGGAGGCTCGTGTGCGTCTAAAGGGTGGTGCCCACCAGGGAGAAGGCAGAGTGGAAGTCCTGAAGGCTGG CACATGGGGAACAGTCTGTGACCGAAAGTGGGACCTGCAGGCAGCCAGTGTGGTGTGTCGAGAACTGGGC TTTGGCACTGCCCGAGAGGCCCTGAGTGGTGCCCGCATGGGGCAGGGCATGGGTGCCATCCACTTGAGTG AAGTTCGGTGCTCTGGCCAGGAGCCCTCCCTTTGGAGATGCCCGTCCAAGAACATCACAGCTGAGGACTG TTCCCACAGCCAGGATGCGGGTGTCCGGTGCAACCTCCCCTACACTGGGGTGGAGACCAAGATCCGACTC AGTGGGGGCCGCAGCCGGTATGAAGGGCGAGTCGAGGTGCAAATAGGGATACCTGGGCATCTACGCTGGG GCCTCATCTGTGGGGATGACTGGGGAACCCTGGAGGCCATGGTGGCCTGTAGGCAACTTGGTCTTGGCTA CGCCAACCACGGCCTGCAGGAGACCTGGTACTGGGACTCAGGGAATGTAACTGAGGTGGTGATGAGCGGA GTGCGTTGCACGGGGTCTGAGCTGTCCCTGAACCAGTGTGCCCATCACAGCAGCCACATCACCTGCAAGA AGACGGGAACCCGCTTCACTGCGGGGGTCATCTGTTCTGAAACTGCTTCAGATCTGCTGCTGCACTCGGC ACTGGTACAGGAAACTGCTTATATTGAAGACCGGCCCTTGCACATGTTGTACTGTGCTGCTGAAGAGAAC TGCCTGGCTAGCTCTGCCCGCTCGGCCAACTGGCCTTATGGCCACCGGCGTCTGCTCCGATTCTCTTCCC AGATCCACAACCTAGGAAGAGCTGACTTTAGGCCCAAGGCTGGGCGCCATTCCTGGGTGTGGCATGAGTG TCATGGGCATTACCACAGTATGGACATCTTCACTCATTATGATATCCTGACCCCCAATGGCACCAAGGTG GCTGAGGGCCACAAAGCTAGTTTCTGTCTAGAAGACACTGAGTGTCAAGAGGATGTCTCCAAGAGGTATG AGTGCGCCAACTTTGGAGAGCAGGGCATCACCGTGGGATGTTGGGATCTCTACAGGCATGACATTGACTG TCAGTGGATCGACATCACAGATGTGAAGCCAGGAAACTACATTCTTCAGGTCGTTATCAACCCAAATTTT GAGGTAGCAGAAAGTGACTTCACCAACAATGCAATGAAGTGTAACTGCAAATACGACGGGCATCGCATCT GGGTGCACAACTGCCACATCGGTGATGCCTTCAGCGAAGAAGCCAACAGGAGGTTTGAGCGCTACCCTGG CCAGACTAGTAACCAGATCGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210480 representing BC011298 Red=Cloning site Green=Tags(s) MRAVSVWYCCPWGLLLLHCLCSFSVGSPSPSISPEKKVGSQGLRFRLAGFPRKPYEGRVEIQRAGEWGTI CDDDFTLQAAHVLCRELGFTEATGWTHSAKYGPGTGRIWLDNLSCRGTEGSVTECASRGWGNSDCTHDED AGVICKDQRLPGFSDSNVIEVEHQLQVEEVRLRPAVEWGRRPLPVTEGLVEVRLPEGWSQVCDKGWSAHN SHVVCGMLGFPGEKRVNMAFYRMLAQKKQHSFGLHSVACVGTEAHLSLCSLEFYRANDTTRCSGGNPAVV SCVLGPLYATFTGQKKQQHSKPQGEARVRLKGGAHQGEGRVEVLKAGTWGTVCDRKWDLQAASVVCRELG FGTAREALSGARMGQGMGAIHLSEVRCSGQEPSLWRCPSKNITAEDCSHSQDAGVRCNLPYTGVETKIRL SGGRSRYEGRVEVQIGIPGHLRWGLICGDDWGTLEAMVACRQLGLGYANHGLQETWYWDSGNVTEVVMSG VRCTGSELSLNQCAHHSSHITCKKTGTRFTAGVICSETASDLLLHSALVQETAYIEDRPLHMLYCAAEEN CLASSARSANWPYGHRRLLRFSSQIHNLGRADFRPKAGRHSWVWHECHGHYHSMDIFTHYDILTPNGTKV AEGHKASFCLEDTECQEDVSKRYECANFGEQGITVGCWDLYRHDIDCQWIDITDVKPGNYILQVVINPNF EVAESDFTNNAMKCNCKYDGHRIWVHNCHIGDAFSEEANRRFERYPGQTSNQIV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC011298
ORF Size	2264 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011298, AAH11298
RefSeq Size	2837 bp
RefSeq ORF	2264 bp
Locus ID	16950
Gene Summary	Protein-lysine 6-oxidase that mediates the oxidation of peptidyl lysine residues to allysine in target proteins (PubMed:26954549). Catalyzes the post-translational oxidative deamination of peptidyl lysine residues in precursors of elastin and different types of collagens, a prerequisite in the formation of cross-links between collagens and elastin (PubMed:26307084). Required for somite boundary formation by catalyzing oxidation of fibronectin (FN1), enhancing integrin signaling in myofibers and their adhesion to the myotendinous junction (MTJ) (PubMed:26954549). Acts as a regulator of inflammatory response by inhibiting differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): acts by interacting with STAT3 in the nucleus and catalyzing both deacetylation and oxidation of lysine residues on STAT3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (By similarity). Also able to catalyze deacetylation of lysine residues on STAT3 (By similarity).[UniProtKB/Swiss-Prot Function]

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