Ezh2 (NM_007971) Mouse Tagged ORF Clone
CAT#: MG210436
- TrueORF®
Ezh2 (tGFP-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2)
ORF Plasmid: DDK
"NM_007971" in other vectors (6)
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CNY 9,792.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Enx-1; Enx1h; KMT6; mKIAA4065 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210436 representing NM_007971
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCAGACTGGGAAGAAATCTGAGAAGGGACCGGTTTGTTGGCGGAAGCGTGTAAAATCAGAGTACA TGAGACTGAGACAGCTCAAGAGGTTCAGAAGAGCTGATGAAGTAAAGACTATGTTTAGTTCCAATCGTCA GAAAATTTTGGAAAGAACTGAAACCTTAAACCAAGAGTGGAAGCAGCGGAGGATACAGCCTGTGCACATC ATGACTTCTGTGAGCTCATTGCGCGGGACTAGGGAGTGTTCAGTCACCAGTGACTTGGATTTTCCAGCAC AAGTCATCCCGTTAAAGACCCTGAATGCAGTCGCCTCGGTGCCTATAATGTACTCTTGGTCGCCCTTACA ACAGAATTTTATGGTGGAAGACGAAACTGTCTTACATAACATTCCTTACATGGGGGATGAAGTTCTGGAT CAGGACGGCACTTTCATTGAAGAACTAATAAAAAATTATGATGGAAAAGTGCATGGTGACAGAGAATGTG GATTTATAAATGATGAAATTTTTGTGGAGTTGGTAAATGCTCTTGGTCAATATAATGATGATGATGATGA CGATGATGGAGATGATCCAGATGAAAGAGAAGAAAAACAGAAAGATCTAGAGGATAATCGAGATGATAAA GAAACTTGCCCACCTCGGAAATTTCCTGCTGATAAAATATTTGAAGCCATTTCCTCAATGTTTCCAGATA AGGGCACCGCAGAAGAACTGAAGGAAAAATATAAAGAACTCACGGAGCAGCAGCTCCCAGGTGCTCTGCC TCCTGAATGTACTCCAAACATCGATGGACCAAATGCCAAATCTGTTCAGAGGGAGCAAAGCTTGCATTCA TTTCATACGCTCTTCTGTCGACGATGTTTTAAGTATGACTGCTTCCTACATCCCTTCCATGCAACACCCA ACACATATAAGAGGAAGAACACAGAAACAGCTTTGGACAACAAGCCTTGTGGACCACAGTGTTACCAGCA TCTGGAGGGAGCTAAGGAGTTTGCTGCTGCTCTTACTGCTGAGCGGATAAAGACACCACCTAAACGCCCA GGGGGCCGCAGAAGAGGAAGACTTCCGAATAACAGTAGCAGACCCAGCACCCCCACCATCAGTGTGCTGG AGTCAAAGGATACAGACAGTGACAGAGAAGCAGGGACTGAAACTGGGGGAGAGAACAACGATAAAGAAGA AGAAGAGAAAAAAGATGAGACGTCCAGCTCCTCTGAAGCAAATTCTCGGTGTCAAACACCAATAAAGATG AAGCCAAATATTGAACCTCCTGAGAATGTGGAGTGGAGTGGTGCTGAAGCCTCCATGTTTAGAGTCCTCA TTGGTACTTACTACGATAACTTTTGTGCCATTGCTAGGCTAATTGGGACCAAAACATGTAGACAGGTGTA TGAGTTTAGAGTCAAGGAGTCCAGTATCATAGCACCTGTTCCCACTGAGGATGTAGACACTCCTCCAAGA AAGAAGAAAAGGAAACATCGGTTGTGGGCTGCACACTGCAGAAAGATACAACTGAAAAAGGACGGCTCCT CTAACCATGTTTACAACTATCAACCCTGTGACCATCCACGGCAGCCTTGTGACAGTTCGTGCCCTTGTGT GATAGCACAAAATTTTTGTGAAAAGTTTTGTCAATGTAGTTCAGAGTGTCAAAACCGCTTTCCTGGATGT CGGTGCAAAGCACAATGCAACACCAAACAGTGTCCATGCTACCTGGCTGTCCGAGAGTGTGACCCTGACC TCTGTCTCACGTGTGGAGCTGCTGACCATTGGGACAGTAAAAATGTATCCTGTAAGAACTGTAGCATTCA GCGGGGCTCTAAAAAGCACTTACTGCTGGCACCGTCTGATGTGGCAGGCTGGGGCATCTTTATCAAAGAT CCTGTACAAAAAAATGAATTCATCTCAGAATACTGTGGGGAGATTATTTCTCAGGATGAAGCAGACAGAA GAGGAAAAGTGTATGACAAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGC AACCCGAAAGGGCAACAAAATTCGTTTTGCTAATCATTCAGTAAATCCAAACTGCTATGCAAAAGTTATG ATGGTTAATGGTGACCACAGGATAGGCATCTTTGCTAAGAGGGCTATCCAGACTGGTGAAGAGTTGTTTT TTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTGGGCATCGAACGAGAAATGGAAATCCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210436 representing NM_007971
Red=Cloning site Green=Tags(s) MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKTMFSSNRQKILERTETLNQEWKQRRIQPVHI MTSVSSLRGTRECSVTSDLDFPAQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLD QDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPDEREEKQKDLEDNRDDK ETCPPRKFPADKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHS FHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRP GGRRRGRLPNNSSRPSTPTISVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKM KPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPVPTEDVDTPPR KKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGC RCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKD PVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVM MVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007971 |
ORF Size | 2238 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007971.1, NP_031997.1 |
RefSeq Size | 2665 bp |
RefSeq ORF | 2241 bp |
Locus ID | 14056 |
UniProt ID | Q61188 |
Gene Summary | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221274 | Ezh2 (untagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1, (10ug) |
CNY 9,690.00 |
|
MR210436 | Ezh2 (Myc-DDK-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 8,192.00 |
|
MR210436L1 | Lenti ORF clone of Ezh2 (Myc-DDK-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 7,700.00 |
|
MR210436L2 | Lenti ORF clone of Ezh2 (mGFP-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 10,592.00 |
|
MR210436L3 | Lenti ORF clone of Ezh2 (Myc-DDK-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 7,700.00 |
|
MR210436L4 | Lenti ORF clone of Ezh2 (mGFP-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 10,592.00 |