Sel1l (BC026816) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AW493766; mKIAA4137; Sel1h
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210395 representing BC026816 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGTCCGCGTCAGGCTGTCGTTGCTGCTGCTCTGCGCGGTGCTCCTGGGCTCGGCAGCCGCGACCT CGGATGACAAAACTAACCAGGATGACTCCTTAGATTCCAAGAGTTCTTTGCCCACAGATGAGTCAGTGAA GGACCACACCACCACGGGCAAAGTAGTTGCTGGCCAGATATTTGTTGATTCTGAAGAAGCAGAAGTGGAA TCCCTTCTTCAGGACGAGGAAGATAGCTCCAAGACCCAGGAGGAAGAGATCAGCTTTTTAGAATCTCCGA ATCCAAGCAGCAAGACCTACGAAGAACTAAAGAGAGTGCGGAAGCCAGTCTTGACTGCCATTGAAGCTGA AGAAGATGCTGCCAAAAGACGACAGATGCAGGAAGCAGAGATGATCTATCAGGCCGGGATGAAGATACTG AATGGAAGCAATAGGAAGAGCCAAAAGAGAGAAGCATATCGGTACCTTCAGAAGGCAGCAGGCATGAATC ACACCAAAGCCCTGGAGAGAGTGTCCTATGCTCTCTTGTTTGGTGATTACCTCACACAGAATATCCAGGC AGCCAAAGAGATGTTTGAGAAACTGACTGAGGAAGGGTCTCCCAAAGGACAGACTGGTCTTGGCTTTCTC TACGCTTCTGGGCTTGGTGTTAATTCAAGTCAGGCAAAGGCTCTTGTATATTATACTTTCGGAGCTCTTG GAGGCAACCTGATAGCCCATATGATTTTGGGTTACCGCTACTGGGCTGGCATCGGAGTCCTCCAGAGTTG TGAGTCGGCACTGACCCATTATCGTCTTGTTGCCAATCATGTTGCTAGTGATATCTCCCTAACTGGAGGC TCTGTAGTCCAGAGAATACGGCTGCCCGATGAAGTGGAAAACCCGGGGATGAACAGTGGGATGCTGGAAG AAGACCTGATTCAGTATTACCAGTTCCTAGCTGAGAAGGGTGACGTCCAAGCACAGGTTGGCCTGGGACA GCTGCATCTGCATGGAGGGCGTGGAGTAGAACAGAATCACCAGAGAGCGTTTGACTACTTCAACTTAGCA GCAAATGCTGGCAATTCACATGCTATGGCCTTCCTGGGAAAGATGTATTCTGAAGGAAGTGACATCGTAC CTCAGAGTAATGAGACGGCACTTCACTACTTTAAGAAAGCTGCTGACATGGGCAACCCCGTGGGACAGAG CGGGCTTGGAATGGCCTACCTCTACGGAAGAGGCGTTCAAGTTAATTATGACCTGGCCCTCAAGTATTTC CAGAAAGCTGCTGAGCAAGGCTGGGTGGACGGGCAGCTGCAGCTCGGCTCTATGTACTACAATGGCATTG GAGTCAAGAGAGATTATAAGCAGGCCTTGAAGTATTTTAATCTGGCTTCTCAAGGAGGCCATATCTTGGC TTTCTATAACCTCGCACAGATGCACGCCAGCGGCACAGGGGTGATGCGGTCCTGTCACACTGCAGTGGAG TTGTTTAAGAATGTGTGTGAGCGAGGTCGCTGGTCAGAGAGACTGATGACTGCCTACAACAGCTATAAGG ATGAGGACTACAATGCTGCAGTGGTCCAGTACCTCCTGCTGGCTGAGCAGGGCTACGAGGTGGCGCAGAG CAACGCAGCCTTCATCCTCGACCAGAGAGAAGCAACCATTGTAGGTGAGAATGAAACTTACCCCAGAGCT TTACTGCATTGGAACAGGGCCGCCTCCCAAGGTTACACTGTGGCTAGAATTAAGCTTGGAGACTACCACT TCTATGGCTTTGGCACTGATGTGGATTATGAGACCGCATTTATTCATTACCGCCTGGCTTCTGAGCAGCA GCACAGCGCCCAAGCTATGTTTAACCTGGGCTACATGCACGAGAAGGGCCTAGGCATTAAACAGGACATT CACCTTGCAAAACGCTTTTATGACATGGCAGCCGAAGCTAGCCCAGATGCACAAGTACCTGTGTTCCTCG CACTCTGCAAATTAGGTGTCGTCTATTTCTTACAGTACATACGGGAAGCAAACATTCGAGATCTATTCAC ACAACTGGATATGGACCAGCTTTTGGGACCCGAGTGGGACCTTTACCTCATGACCATCATTGCACTGCTC TTGGGTACAGTCATAGCTTACAGGCAGCGACAGCACCAGGACATACCAGTTCCCAGGCCCCCAGGGCCAC GGCCGGCTCCTCCCCAGCAGGAAGGACCACCAGAGCAGCAGCCACCACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210395 representing BC026816 Red=Cloning site Green=Tags(s) MQVRVRLSLLLLCAVLLGSAAATSDDKTNQDDSLDSKSSLPTDESVKDHTTTGKVVAGQIFVDSEEAEVE SLLQDEEDSSKTQEEEISFLESPNPSSKTYEELKRVRKPVLTAIEAEEDAAKRRQMQEAEMIYQAGMKIL NGSNRKSQKREAYRYLQKAAGMNHTKALERVSYALLFGDYLTQNIQAAKEMFEKLTEEGSPKGQTGLGFL YASGLGVNSSQAKALVYYTFGALGGNLIAHMILGYRYWAGIGVLQSCESALTHYRLVANHVASDISLTGG SVVQRIRLPDEVENPGMNSGMLEEDLIQYYQFLAEKGDVQAQVGLGQLHLHGGRGVEQNHQRAFDYFNLA ANAGNSHAMAFLGKMYSEGSDIVPQSNETALHYFKKAADMGNPVGQSGLGMAYLYGRGVQVNYDLALKYF QKAAEQGWVDGQLQLGSMYYNGIGVKRDYKQALKYFNLASQGGHILAFYNLAQMHASGTGVMRSCHTAVE LFKNVCERGRWSERLMTAYNSYKDEDYNAAVVQYLLLAEQGYEVAQSNAAFILDQREATIVGENETYPRA LLHWNRAASQGYTVARIKLGDYHFYGFGTDVDYETAFIHYRLASEQQHSAQAMFNLGYMHEKGLGIKQDI HLAKRFYDMAAEASPDAQVPVFLALCKLGVVYFLQYIREANIRDLFTQLDMDQLLGPEWDLYLMTIIALL LGTVIAYRQRQHQDIPVPRPPGPRPAPPQQEGPPEQQPPQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026816
ORF Size	2222 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026816, AAH26816
RefSeq Size	3540 bp
RefSeq ORF	2222 bp
Locus ID	20338
Gene Summary	Plays a role in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:25066055, PubMed:24453213). Enhances SYVN1 stability (PubMed:24453213). Plays a role in LPL maturation and secretion (PubMed:25066055). Required for normal differentiation of the pancreas epithelium, and for normal exocrine function and survival of pancreatic cells (PubMed:20170518, PubMed:24453213). May play a role in Notch signaling (PubMed:20170518).[UniProtKB/Swiss-Prot Function]

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