Smurf1 (BC029097) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4930431E10Rik; mKIAA1625
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210325 representing BC029097 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAACCCCGGGACCCGTAGGAACGGCTCCAGCATCAAGATCCGTCTGACAGTATTGTGTGCCAAGA ACCTTGCAAAGAAAGACTTCTTCAGACTCCCCGACCCCTTTGCCAAGATTGTTGTGGACGGCTCTGGGCA GTGCCACTCAACCGACACTGTGAAAAACACCCTGGACCCAAAGTGGAGCCAGCACTATGACCTGTATGTT GGGAAAACGGACTCGATAACCATCAGTGTGTGGAACCACAAGAAGATCCACAAGAAGCAGGGGGCTGGCT TCCTGGGCTGTGTGCGGCTGCTCTCCAATGCCATCAGCAGATTGAAAGACACTGGCTACCAGCGTTTGGA TCTATGCAAACTAAATCCCTCAGATACTGATGCAGTTCGTGGCCAAATAGTGGTCAGTTTACAGACCCGA GACAGAATAGGCGGTGGAGGGTCAGTGGTGGACTGCAGAGGGCTGCTGGAGAACGAAGGAACAGTGTATG AAGACTCAGGCCCTGGAAGGCCGCTCAGCTGCCTCATGGAGGAACCTGCCCCGTATACAGATGGTACTGG TGCAGCAGCAGGAGGCGGGAACTGCAGGTTTGTGGAGTCTCCAAGCCAAGATCAGAGACTCCTGGTACAG CGACTCCGAAATCCTGAGGTTCGAGGGCCCTTACAGACACCCCAGAACCGACCACATGGCCACCAGTCGC CAGAGCTGCCTGAAGGCTATGAGCAAAGGACAACAGTGCAGGGACAAGTTTACTTTTTGCACACGCAGAC TGGAGTCAGCACATGGCATGACCCCAGGATCCCCAGAGACCTTAACAGTGTGAACTGCGATGAACTTGGG CCACTGCCTCCAGGCTGGGAAGTCCGAAGCACAGTGTCGGGAAGAATCTATTTTGTAGATCACAATAATA GGACAACCCAGTTTACAGATCCACGGCTTCACCACATCATGAATCACCAGTGCCAACTCAAGGAGCCCAG CCAGCCGCTGCAGCTGCCCAGTGAGGGCTCCGTGGAGGACGAGGAGCTTCCTGCCCAGAGATATGAGAGG GACTTAGTCCAGAAGCTCAAAGTTCTCAGGCACGAGCTCTCTCTTCAGCAGCCCCAGGCTGGTCACTGTC GAATAGAAGTCTCCAGAGAAGAGATATTTGAGGAGTCGTATCGCCAGATCATGAAGATGCGGCCAAAAGA CCTAAAGAAGCGCCTCATGGTGAAGTTCCGAGGGGAGGAAGGTTTGGACTATGGTGGAGTGGCTCGGGAG TGGCTGTATTTGTTGTGCCATGAAATGTTGAACCCGTACTATGGACTCTTCCAGTATTCCACGGACAATA TTTACACACTGCAGATCAACCCAGATTCTTCTATCAACCCTGACCATCTGTCTTACTTCCACTTTGTGGG TCGCATCATGGGTCTGGCTGTGTTCCACGGACACTACATAAATGGGGGTTTCACAGTTCCGTTCTACAAG CAGCTCCTGGGGAAGCCAATCCAGCTGTCGGACCTGGAGTCCGTGGACCCAGAACTGCACAAGAGCTTGG TGTGGATTCTAGAGAATGACATCACGCCTGTGTTGGATCATACCTTCTGCGTGGAGCACAACGCTTTCGG GCGGATTCTCCAGCATGAACTGAAACCCAATGGCAGAAATGTGCCTGTCACTGAGGAGAACAAGAAGGAA TACGTCCGGCTGTATGTGAACTGGAGGTTTATGAGAGGAATCGAAGCCCAGTTCTTAGCACTTCAGAAGG GGTTTAACGAACTCATTCCCCAACACTTGCTGAAGCCCTTTGACCAGAAGGAACTAGAGCTGATAATAGG TGGGCTGGATAAGATAGACCTGAACGACTGGAAGTCCAACACCCGGCTGAAACACTGTGTGGCAGACAGC AACATCGTCAGGTGGTTCTGGCAGGCGGTGGAGACCTTCGATGAGGAGAGGAGAGCCAGACTCCTGCAGT TTGTGACAGGATCCACAAGAGTTCCTCTCCAAGGCTTCAAGGCTCTGCAAGGCGCGGCAGGGCCCCGGCT GTTCACCATTCACCTGATAGACGCCAATACAGACAACCTGCCCAAGGCCCATACCTGCTTTAATCGGATC GACATCCCACCCTATGAGTCCTATGAGAAGCTCTATGAGAAGCTGCTGACAGCGGTGGAGGAAACCTGTG GCTTTGCAGTGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210325 representing BC029097 Red=Cloning site Green=Tags(s) MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWSQHYDLYV GKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTR DRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQ RLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELG PLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYER DLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVARE WLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYK QLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKE YVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADS NIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRI DIPPYESYEKLYEKLLTAVEETCGFAVE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC029097
ORF Size	2186 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029097, AAH29097
RefSeq Size	2765 bp
RefSeq ORF	2186 bp
Locus ID	75788
Gene Summary	E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway (By similarity). Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function]

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