Zfp219 (NM_027248) Mouse Tagged ORF Clone

CAT＃: MG210308

TrueORF^®

Zfp219 (tGFP-tagged) - Mouse zinc finger protein 219 (Zfp219)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_027248" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,370.00

货期*

4周

规格

Product images

经常一起买 (4)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2010302A17Rik; Znf219
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210308 representing NM_027248 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGTTCACGTCCCCGCATCCTGGTCGGCCACCTAGAGCCTTCCCCACCAGCCTTCGACGGCGAGC TGGATCTGCAGCGCTACTCCAACGGACCAGGTGTCAGCGGGACTCCAGGACCCGGCTCGCCTGGGATGGG AGCAGTGGGTTGGTCTGAGACTCGTGCAGGCGAACGGCGTTTCCCTTGTCCTGTGTGCGGAAAGCGCTTC CGATTCAATTCCATCTTGGCTTTGCACCTGAGAGCCCACCCGGGCGCCCAAGCCTTCCAGTGCCCACATT GCGGCCACCGCGCAGCGCAGCGGGCTCTGCTGCGCTCACACCTCCGAACGCACCAGCCGGAGCGTCCACG CAGCCCTGCTGCACGGCTGTTGCTGGAGTTGGAGGAACGCGCCCTGCTTCGCGAAGCCCGACTCGGGAGA GCCAGAAGCTCAGGCGGCATGCAGTCCAGCCCTGCCGCAGAGGGCCTGGCTCGCCCTCAGGTCCCTTCCT CATCTGCCTTCCGTTGCCCTTTCTGCAAAGGCAAGTTTCGCACCTCAGCGGAGCGGGAACGCCACCTGCA TATCCTGCACAGGCCCTGGAAGTGCAGCTTGTGCAGTTTTGGCTCCAGCCAGGAGGAGGAGTTGCTGCAC CACAGTCTGACGGCCCACGGGGCTTCCGAGCGCCCCCTGGCGGCTACATCTACGCCTGAACCCCCGCCTC CACCCCAGCAAGAACCCAGATCTGCCCTTGAGCCTGAGCCTGAGCCTGAACCTAGGCCAGAGCCTGACCG GGAGGCAAACCCTGCCCCAACTCCTGCACCTCCGGAGGAGCCCCCTGCGCCACCTGAATTCCGTTGCCAG GTGTGCGGCCAGAGCTTTACACAGTCCTGGTTTCTCAAGGGTCACATGCGCAAGCACAAGGCCTCCTTTG ATCATGCGTGCCCTGTATGTGGTCGCTGCTTCAAGGAGCCCTGGTTCCTTAAGAACCACATGAAGGTGCA CACCAGCAAGCTGGGTCCTTTGCGTGCTCCAGGCCCTGGCTCTGCACCTGCCAGGGCCCCTCAGCCTCCC GACCTGAGCCTACTGGCATATGAGCCACTGGGCCCTGCACTCCTCCTGGCCCCAGCACCCGCTCCGGCTG AGCGCCGAGAGCCCCCAAGCCTCTTAGGCTACCTGAGTGTACGAGCTGGGGAAGTACGACCCAATGGTGA GGGTGCTGACCCTGGAGGTGGCCGAAGCTATGGAGGGTTCCGCCCACTGCCTTCAGCTCTTCCCAACCGG GCTCGGCGACACCGTACAGAGGAACCAGAAGAGGAAGAAGAAGTGGTGGAGGCTGAAGAGGAGAGCTGGG CCCGAGGCAGGTCGCTGGGCTCTCTGACTTCCCTGCACCCCAACCCAGGTGAGGGGTCAGGACAGCCTGC ACCTGCTGCGGGGACCCAGGCGAGATCCACGGCCACCCAAGAAGAAAACGGGCTGCTGGTTGGAGGGACA CGATCTGAAGCGGGCCGTGGGGCCACTGGCAAGGACTGCCCCTTCTGTGGAAAATCTTTCCGCTCGGCGC ATCACTTAAAAGTGCATCTCCGTGTGCACACAGGTGAGCGTCCCTACAAGTGTCCACACTGCGACTATGC AGGTACCCAGTCGGGCTCGCTCAAGTATCACCTTCAGCGTCACCACCGAGAGCAGAGGAGCAGTGCGGGT CCTGGGCCTCCCCCAGAACCCCCGCCACCTTCCCAGCGGGGCTCACTGCAGCCGCAGTCAGGAGCCAAGC CAACTCAGGCCTCAGCCACCTGGGTAGAGGGCACTGCAAGTACCCGGCCTCCTTCGAGCAGCACCGGACC AGGGTCCCGTAGGAAGCCTGCTAGCCCTGGGAGGACCCTGCGAAACGGGCGAGGTGGTGAAGCCGAACCC CTGGACCTGTCCCTACGGGCGGGCCCGGGAGGTGAGGCCGGGGCAGGGGGTGCCCTTCACCGCTGCCTCT TCTGTCCCTTTGCCACTGGAGCTCCTGAGCTCATGGCCTTGCATCTGCAAGTACATCATAGCCGTCGTGC TCGGGGCCGCCGGCAGCCCCGAGCCGACACGTCTCCAACCTATGTCCGGGCACCATCAGGAGAGACCCCT CCCAGTCCTCCACTAGAAGAGGAGGGCAGCCCAGGGCTGTCTAGATCCGGAGAGGCAGGTCTTGGGGGGC AAGAACGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210308 representing NM_027248 Red=Cloning site Green=Tags(s) MEGSRPRILVGHLEPSPPAFDGELDLQRYSNGPGVSGTPGPGSPGMGAVGWSETRAGERRFPCPVCGKRF RFNSILALHLRAHPGAQAFQCPHCGHRAAQRALLRSHLRTHQPERPRSPAARLLLELEERALLREARLGR ARSSGGMQSSPAAEGLARPQVPSSSAFRCPFCKGKFRTSAERERHLHILHRPWKCSLCSFGSSQEEELLH HSLTAHGASERPLAATSTPEPPPPPQQEPRSALEPEPEPEPRPEPDREANPAPTPAPPEEPPAPPEFRCQ VCGQSFTQSWFLKGHMRKHKASFDHACPVCGRCFKEPWFLKNHMKVHTSKLGPLRAPGPGSAPARAPQPP DLSLLAYEPLGPALLLAPAPAPAERREPPSLLGYLSVRAGEVRPNGEGADPGGGRSYGGFRPLPSALPNR ARRHRTEEPEEEEEVVEAEEESWARGRSLGSLTSLHPNPGEGSGQPAPAAGTQARSTATQEENGLLVGGT RSEAGRGATGKDCPFCGKSFRSAHHLKVHLRVHTGERPYKCPHCDYAGTQSGSLKYHLQRHHREQRSSAG PGPPPEPPPPSQRGSLQPQSGAKPTQASATWVEGTASTRPPSSSTGPGSRRKPASPGRTLRNGRGGEAEP LDLSLRAGPGGEAGAGGALHRCLFCPFATGAPELMALHLQVHHSRRARGRRQPRADTSPTYVRAPSGETP PSPPLEEEGSPGLSRSGEAGLGGQER TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_027248
ORF Size	2178 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_027248.1, NP_081524.1
RefSeq Size	2938 bp
RefSeq ORF	2181 bp
Locus ID	69890
UniProt ID	Q6IQX8
Gene Summary	Transcriptional regulator (PubMed:20940257). Recognizes and binds 2 copies of the core DNA sequence motif 5'-GGGGG-3' (PubMed:20940257). Binds to the HMGN1 promoter and may repress HMGN1 expression (By similarity). Regulates SNCA expression in primary cortical neurons (By similarity). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with SOX9 (PubMed:20940257). Plays a role in chondrocyte differentiation (PubMed:20940257).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC221066	Zfp219 (untagged) - Mouse zinc finger protein 219 (Zfp219), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,310.00
MR210308	Zfp219 (Myc-DDK-tagged) - Mouse zinc finger protein 219 (Zfp219)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,320.00 CNY 5,800.00
MR210308L3	Lenti ORF clone of Zfp219 (Myc-DDK-tagged) - Mouse zinc finger protein 219 (Zfp219)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
MR210308L4	Lenti ORF clone of Zfp219 (mGFP-tagged) - Mouse zinc finger protein 219 (Zfp219)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00

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