Dnm1l (BC079635) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	6330417M19Rik; AI450666; Dlp1; Dnmlp1; Drp1; python
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210122 representing BC079635 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGCTGATCCCGGTCATCAATAAGCTGCAGGACGTCTTCAACACAGTGGGTGCGGACATCATCC AGCTGCCTCAGATCGTCGTAGTGGGAACGCAGAGCAGTGGGAAGAGCTCAGTGCTGGAAAGCCTAGTGGG CAGGGACCTTCTTCCCAGAGGAACTGGTGTGGTCACCCGGAGACCTCTCATTCTGCAGCTAGTCCACGTT TCACCAGAAGATAAAAGAAAAACAACAGGAGAAGAAAATGGAGTTGAAGCAGAAGAATGGGGTAAATTTC TTCACACCAAAAACAAGCTTTACACAGATTTTGATGAAATTCGACAAGAAATTGAAAATGAAACTGAAAG AATTTCAGGAAATAATAAGGGGGTAAGCCCTGAGCCAATCCATCTCAAGGTTTTCTCGCCCAACGTTGTC AACCTGACACTTGTGGATTTACCGGGAATGACCAAAGTACCTGTAGGCGATCAGCCCAAGGACATCGAGC TTCAGATCAGAGAACTTATTCTTCGGTTCATCAGTAATCCCAATTCCATTATCCTCGCCGTCACTGCTGC AAATACAGATATGGCAACATCAGAAGCACTCAAGATTTCCAGAGAGGTAGATCCAGATGGGCGCAGAACT CTAGCTGTAATCACTAAACTTGATCTCATGGATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGG TTATTCCAGTCAAGCTTGGAATAATTGGAGTAGTTAACAGAAGCCAACTGGATATTAACAATAAGAAGAG TGTAACTGATTCAATCCGTGATGAGTATGCTTTTCTTCAAAAGAAGTACCCATCTCTGGCCAACAGAAAT GGAACAAAGTATCTTGCTAGGACCCTGAATAGGTTACTTATGCATCATATCAGAGATTGTTTACCAGAGC TGAAAACAAGAATAAATGTCTTAGCTGCTCAGTATCAGTCTCTTCTAAATAGCTATGGTGAACCGGTGGA TGATAAAAGTGCTACTTTACTCCAGCTTATTACCAAATTTGCCACAGAGTATTGTAACACGATTGAAGGA ACCGCAAAGTACATTGAAACTTCTGAGCTATGCGGTGGTGCTAGGATTTGTTATATTTTCCATGAGACTT TCGGGCGAACCTTAGAATCTGTGGACCCACTAGGTGGCCTTAACACTATTGACATCCTGACTGCCATCAG AAATGCTACTGGCCCCCGTCCTGCTTTATTTGTGCCTGAAGTTTCATTTGAGTTACTGGTCAAACGTCAG ATTAAGCGTCTAGAAGAGCCCAGCCTACGGTGTGTGGAGCTGGTCCATGAGGAGATGCAGAGGATCATTC AGCATTGTAGCAATTACAGCACACAGGAATTGTTACGGTTCCCTAAACTTCACGATGCCATAGTTGAAGT AGTGACCTGTCTTCTTCGTAAAAGGTTGCCCGTGACAAATGAAATGGTGCATAACTTAGTGGCAATTGAG CTAGCGTATATCAACACAAAACACCCCGACTTTGCTGATGCCTGTGGGCTAATGAACAATAATATAGAGG AACAAAGAAGAAACAGGCTAGCAAGAGAGCTGCCTTCAGCTGGATCACGGGACAAGGTCCCATCTGCAGG TGGTGGGATTGGAGACGGTGGTCAGGAACCAACAACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAA GCTGAAGAATTACTTGCTGAAGAAAAATCAAAACCAATTCCAATTATGCCAGCAAGTCCACAGAAAGGCC ATGCTGTCAATTTGCTAGATGTGCCAGTTCCAGTTGCAAGAAAACTGTCTGCCCGAGAACAGCGAGATTG TGAGGTTATTGAAAGACTTATCAAATCATATTTTCTAATTGTCAGAAAGAATATTCAAGACAGTGTCCCA AAGGCAGTAATGCATTTTTTGGTTAATCATGTGAAAGATACTCTTCAGAGTGAACTGGTAGGGCAGCTGT ATAAGTCATCCTTATTAGATGACCTTCTGACTGAATCCGAGGACATGGCCCAACGAAGAAAAGAAGCAGC GGATATGCTGAAGGCATTACAAGGAGCCAGTCAAATTATTGCTGAAATCCGAGAGACTCATCTTTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210122 representing BC079635 Red=Cloning site Green=Tags(s) MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLILQLVHV SPEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKVFSPNVV NLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRT LAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRN GTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEG TAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQ IKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIE LAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAGSRDKVPSAGGGIGDGGQEPTTGNWRGMLKTSK AEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVP KAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC079635
ORF Size	2099 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC079635, AAH79635
RefSeq Size	3872 bp
RefSeq ORF	2099 bp
Locus ID	74006
Gene Summary	This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013]

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