Hbs1l (BC010251) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2810035F15Rik; AI326327; eRFS
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210012 representing BC010251 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGCATCGGAACGTCCGGGGATATAACTACGATGAAGATTTTGAAGATGATGATCTGTATGGGC AGTCTGTGGAGGATGACTACTGTATCTCACCATCAACAGCCGCTCAGTTCATTTACTCACGCCGTGACAA CCCTGAAGAAGAATATGGCTATGAAGATCTGAGAGAGTCTTCTAATTCTCTTTTGAATCACCAATTAAGT GAAATTGACCAAGCTCGCCTGTATTCATGCCTTGATCACATGAGAGAGGTACTGGGGGACGCTGTGCCTG ATGACATACTGACTGAGGCAATTCTGAAACACAAATTTGATGTGCAGAAGGCTTTGTCCGTGGTTCTGGA ACAAGATGGTGTGCAGCCTTGGAAGGAGAAGAGTGAGCGAGCAGTGTGTGCAGGACAGCCTTCAAAAGGA AAATCAGTTATATCTCGGTCATCCCAGAGTGAATCTGAAATTGTGCCAAAAGTTGCTAAAATGACTGTAT CCGGGAAGAAGCAGACCATGGGATTTGAAGTGCCTGGCTTAACTTCTGAGGAAAATGGACTCAGTGTGCG CGCTCCTCACAAAGGGCCTCCTGGTGATGATGTGAGCGTAGCTTCTCCTAACATTCCTGAGACCGGCACT CCAAAATCTGCCCTCCCACCTCCTTCCCTTCAAACATCAGAAGAGCTGGGCTCCACTCCGACACCAGTGA GAAAGTCTGGCAAGCTGAGGCAGCAGATAGACGTGAAAGCGGAATTGGAAAAGCGGCAGGGCGGGAAGCA GCTCCTCAACTTAGTGGTCATTGGTCATGTTGATGCTGGGAAAAGTACTCTGATGGGCCATATGCTTTAT CTTCTGGGTAATGTAAACAAAAGAACTATGCATAAGTATGAGCAAGAATCTAAAAAGGCTGGCAAGGCTT CATTTGCATATGCGTGGGTCTTGGATGAAACTGGAGAAGAAAGGGAAAGGGGAGTAACAATGGACGTCGG AATGACGAAGTTTGAAACCACAACCAAAGTTATTACCTTAATGGATGCTCCAGGCCATAAGGATTTCATT CCAAACATGATCACAGGAGCAGCCCAGGCCGATGTCGCTGTCCTGGTCGTTGATGCCAGCAGAGGAGAGT TTGAAGCTGGATTTGAGACGGGAGGACAGACCCGAGAGCATGGCCTTTTGGTCCGATCTCTTGGAGTGAC ACAGCTTGCTGTGGCTGTCAATAAGATGGATCAGGTAAATTGGCAACAAGAAAGATTTCAAGAGATTACT GGAAAACTCGGGCACTTTCTTAAGCAAGCAGGTTTTAAGGAGAGTGATGTAGCTTTTATCCCGACGAGCG GTCTGAGTGGTGAGAATTTAACCGCAAGGTCACAGTCCAGTGACCTCACGACGTGGTACAAAGGGATGTG CTTATTGGAGCAGATTGATTCCTTCAAGCCCCCTCAGCGCTCGATTGACAAACCTTTTAGATTATGTGTG TCTGATGTCTTCAAAGATCAAGGATCTGGCTTTTGTGTGACTGGTAAAATTGAAGCTGGTTATATCCAGA CTGGTGACCGACTGTTAGCCATGCCACCCAATGAAACCTGTACTGCTAAAGGAATCACTCTGCACGATGA GCCTGTCGATTGGGCAGCAGCAGGCGATCACGTAAATCTTACCTTGGTTGGGATGGACATCATCAAAATC AATGTCGGCTGCATATTTTGTGCCCCCAAAGAACCCATTAAGGCTTGCACCCGCTTCAGAGCCCGAATCC TCGTCTTCAATATTGAAGTTCCGATCACTAAAGGGTTTCCTGTGCTGTTACACTACCAAACCGTCAGTGA GCCTGCTGTTATTAAGCGATTGATTAGTGTCCTAAACAAAAGTACTGGTGAAGTCACAAAGAAGAAGCCC AAGTTGCTGACCAAGGGCCAGAACGCCTTGGTAGAGCTGCAGACACAAAGACCAGTGGCTCTTGAGCTCT ACAAAGACTTCAAGGAGCTGGGGCGGTTTATGCTGCGTTATGGTGGCTCCACAGTAGCTGCCGGTGTTGT CACTGAGATAAAAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210012 representing BC010251 Red=Cloning site Green=Tags(s) MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDNPEEEYGYEDLRESSNSLLNHQLS EIDQARLYSCLDHMREVLGDAVPDDILTEAILKHKFDVQKALSVVLEQDGVQPWKEKSERAVCAGQPSKG KSVISRSSQSESEIVPKVAKMTVSGKKQTMGFEVPGLTSEENGLSVRAPHKGPPGDDVSVASPNIPETGT PKSALPPPSLQTSEELGSTPTPVRKSGKLRQQIDVKAELEKRQGGKQLLNLVVIGHVDAGKSTLMGHMLY LLGNVNKRTMHKYEQESKKAGKASFAYAWVLDETGEERERGVTMDVGMTKFETTTKVITLMDAPGHKDFI PNMITGAAQADVAVLVVDASRGEFEAGFETGGQTREHGLLVRSLGVTQLAVAVNKMDQVNWQQERFQEIT GKLGHFLKQAGFKESDVAFIPTSGLSGENLTARSQSSDLTTWYKGMCLLEQIDSFKPPQRSIDKPFRLCV SDVFKDQGSGFCVTGKIEAGYIQTGDRLLAMPPNETCTAKGITLHDEPVDWAAAGDHVNLTLVGMDIIKI NVGCIFCAPKEPIKACTRFRARILVFNIEVPITKGFPVLLHYQTVSEPAVIKRLISVLNKSTGEVTKKKP KLLTKGQNALVELQTQRPVALELYKDFKELGRFMLRYGGSTVAAGVVTEIKE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC010251
ORF Size	2048 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010251, AAH10251
RefSeq Size	2742 bp
RefSeq ORF	2048 bp
Locus ID	56422
Gene Summary	Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway. In the presence of ABCE1 and PELO, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes. Together with PELO and in presence of ABCE1, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway.[UniProtKB/Swiss-Prot Function]

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