Hbs1l (BC010251) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810035F15Rik; AI326327; eRFS
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC010251 TTCCGCCCGCGGTCTGCGCCTCGTCGGTTTCTGAGCCGAGTTTTTCTGGAAGGGACGGCGGCAGGCGCCG TCTGTGCGCGATGGCCCGGCATCGGAACGTCCGGGGATATAACTACGATGAAGATTTTGAAGATGATGAT CTGTATGGGCAGTCTGTGGAGGATGACTACTGTATCTCACCATCAACAGCCGCTCAGTTCATTTACTCAC GCCGTGACAACCCTGAAGAAGAATATGGCTATGAAGATCTGAGAGAGTCTTCTAATTCTCTTTTGAATCA CCAATTAAGTGAAATTGACCAAGCTCGCCTGTATTCATGCCTTGATCACATGAGAGAGGTACTGGGGGAC GCTGTGCCTGATGACATACTGACTGAGGCAATTCTGAAACACAAATTTGATGTGCAGAAGGCTTTGTCCG TGGTTCTGGAACAAGATGGTGTGCAGCCTTGGAAGGAGAAGAGTGAGCGAGCAGTGTGTGCAGGACAGCC TTCAAAAGGAAAATCAGTTATATCTCGGTCATCCCAGAGTGAATCTGAAATTGTGCCAAAAGTTGCTAAA ATGACTGTATCCGGGAAGAAGCAGACCATGGGATTTGAAGTGCCTGGCTTAACTTCTGAGGAAAATGGAC TCAGTGTGCGCGCTCCTCACAAAGGGCCTCCTGGTGATGATGTGAGCGTAGCTTCTCCTAACATTCCTGA GACCGGCACTCCAAAATCTGCCCTCCCACCTCCTTCCCTTCAAACATCAGAAGAGCTGGGCTCCACTCCG ACACCAGTGAGAAAGTCTGGCAAGCTGAGGCAGCAGATAGACGTGAAAGCGGAATTGGAAAAGCGGCAGG GCGGGAAGCAGCTCCTCAACTTAGTGGTCATTGGTCATGTTGATGCTGGGAAAAGTACTCTGATGGGCCA TATGCTTTATCTTCTGGGTAATGTAAACAAAAGAACTATGCATAAGTATGAGCAAGAATCTAAAAAGGCT GGCAAGGCTTCATTTGCATATGCGTGGGTCTTGGATGAAACTGGAGAAGAAAGGGAAAGGGGAGTAACAA TGGACGTCGGAATGACGAAGTTTGAAACCACAACCAAAGTTATTACCTTAATGGATGCTCCAGGCCATAA GGATTTCATTCCAAACATGATCACAGGAGCAGCCCAGGCCGATGTCGCTGTCCTGGTCGTTGATGCCAGC AGAGGAGAGTTTGAAGCTGGATTTGAGACGGGAGGACAGACCCGAGAGCATGGCCTTTTGGTCCGATCTC TTGGAGTGACACAGCTTGCTGTGGCTGTCAATAAGATGGATCAGGTAAATTGGCAACAAGAAAGATTTCA AGAGATTACTGGAAAACTCGGGCACTTTCTTAAGCAAGCAGGTTTTAAGGAGAGTGATGTAGCTTTTATC CCGACGAGCGGTCTGAGTGGTGAGAATTTAACCGCAAGGTCACAGTCCAGTGACCTCACGACGTGGTACA AAGGGATGTGCTTATTGGAGCAGATTGATTCCTTCAAGCCCCCTCAGCGCTCGATTGACAAACCTTTTAG ATTATGTGTGTCTGATGTCTTCAAAGATCAAGGATCTGGCTTTTGTGTGACTGGTAAAATTGAAGCTGGT TATATCCAGACTGGTGACCGACTGTTAGCCATGCCACCCAATGAAACCTGTACTGCTAAAGGAATCACTC TGCACGATGAGCCTGTCGATTGGGCAGCAGCAGGCGATCACGTAAATCTTACCTTGGTTGGGATGGACAT CATCAAAATCAATGTCGGCTGCATATTTTGTGCCCCCAAAGAACCCATTAAGGCTTGCACCCGCTTCAGA GCCCGAATCCTCGTCTTCAATATTGAAGTTCCGATCACTAAAGGGTTTCCTGTGCTGTTACACTACCAAA CCGTCAGTGAGCCTGCTGTTATTAAGCGATTGATTAGTGTCCTAAACAAAAGTACTGGTGAAGTCACAAA GAAGAAGCCCAAGTTGCTGACCAAGGGCCAGAACGCCTTGGTAGAGCTGCAGACACAAAGACCAGTGGCT CTTGAGCTCTACAAAGACTTCAAGGAGCTGGGGCGGTTTATGCTGCGTTATGGTGGCTCCACAGTAGCTG CCGGTGTTGTCACTGAGATAAAAGAGTGACGGTCCGGTCCTCATCTCACCATGCTTCCAGAGGAGCGAGT CAGCCCCTGGGGCACTCAGTGGTGCAGCTAAGGAAACAGCATGCAGTCATGATTGTTAGATGAGAGACAC TAAAGTACAGTTAGCTGCCAACAAGTATTAAGGATTACTCTGCAAAACTTCCCAGTTGCCAGCTGCCAGA GGCCAGCACTGAACTGCCATTTCTTCCCTTTCGGGGTGGCAGTAAATATGTTTGTTGTGCTGAGACTTGT GGGAGGTATCACAAGACACTTAACTCAGTGGACTCGACTTCCAGCCAAACCAAATCCAAACGCTGTTCTG TTTCCATTCTCCTGCTGTGTGTGTTCTAAGGAAGCCTTGAACCTTAGTGGGGCTTGGAGAGTCCGTCTTT GACCTGGTGCTTTTTTTAGTTAACACTAAGAAAAGATTTACTGAAGGTAACAGTGAAGCTGTTTTGTGCT GAAAATAAAACCGTCCATCAAGCCTGCCTTCTGGAGTATAATGCGAAGTGACAGAAATCTGTTCTTAAGT AACAAAGATGCTACTTAAGTTGAGAAGCCAGCTAATAAAATGCCTTAGTTTGAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC010251
Insert Size	2049 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010251, AAH10251
RefSeq Size	2742 bp
RefSeq ORF	2049 bp
Locus ID	56422
Gene Summary	Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway. In the presence of ABCE1 and PELO, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes. Together with PELO and in presence of ABCE1, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.