Tbc1d14 (BC042515) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2810413P16Rik; AU043625; C86258; D5Ertd110e; mKIAA1322
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209982 representing BC042515 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATCCTAGATGGTCGGCAAGGAGACTCCCTTCAGGACCTACAACACCTTAGTATCAAGGCGGCTC CCAGATCCCTTTCAGTGCCTGAATACGGACCTTCACTAAAACTTGGTGCTTTGGAAGATCGACACAGCCT TCAATCAGTGGACTCGGGCATTCCTACCCTGGAGATTGGCAACCCAGAGCCTGTTCCCTGCAGTGTGGTC CATGTGAAGAGAAAGCAGTCTGAGTCAGAGATCGTCCCGGAGCGGGCCTTCCAGAGTGCATGCCCCCTGC CGTCCTGCACACCCTCAGCTCCCACCTGCAGCGAGCGGGAGCAGGTTGTGCGGAAGTCTTCCACGTTTCC TAGGACAGGCTATGACTCAGTGAAACTCTACAGCCCCACCTCCAAAGCCTTGAGCCGAAGTGACAATGTC TCTGTCTGCAGTGTGTCTAGTCTTGGCACAGAGCTGTCAACTACGTTGTCGGTCAGCAATGAGGACATCT TGGACCTCATGGTCACGAGCAATTCCAGCGCCATTGTGACCCTGGAGAATGATGATGACCCACAGTTTAC TGACGTCACCCTGAGCTCCATCAATGAAACCAGCGACTTACACCAGCAGGACTGTGTTGCTGAGACTGAG GAGGGGAGGAAATTGAAACTATTGCAACCATTCAGTCACTTCTTTACAAGGAATTTGCTTGCTAGAAAAC AAAATGCAAGGCTTGATAGACAGAGAGACTTGGGATGGAAGCTGTTTGGAAAAGTCCCACTCCGAGAGAC TGCTCAGAAAGATTCAAAGAAGACCCAGAAGGAATATGAAGACAAGGCTGGAAGACCGAGCAGGCCACCC TCTCCCAAGCAGAATGTGAGGAAGAACCTGGATTTTGAGCCACTGTCCACCACGGCGCTCATCCTCGAGG ACAGACCTGCAAATCTCCCAGCCAAGCCAGCTGAAGAAGCTCAAAAGCACAGACAGCAGTATGAAGAGAT GGTGCTGCAGGCCAAGAAACGAGAGCTTAAAGAAGCCCAGCGTCGGAGGAAGCAGCTGGAGGAAAGGTGC AAGGTTGAAGAGAGCATTGGGAACGCTGTCCTCACCTGGAACAATGAGATCTTGCCTAACTGGGAAACCA TGTGGTGCTCTAAGAAGGTTCGAGACTTGTGGTGGCAGGGAATCCCTCCGAGTGTAAGAGGCAAAGTTTG GAGCTTGGCCATTGGCAACGAGTTGAACATCACTCACGAGCTCTTTGACATCTGTCTTGCTCGGGCCAAG GAGCGGTGGCGGTCCCTCAGCACTGGAGGCTCAGAAGTGGAGAATGAAGATGCTGGGTTTTCAGCAGCAG ACAGAGAGGCCAGTCTGGAGCTTATTAAACTGGACATTTCTAGAACATTTCCTAATCTCTGCATTTTCCA GCAAGGTGGTCCGTATCATGACATGTTGCACAGTATTTTGGGCGCTTATACTTGTTACCGGCCGGATGTG GGTTATGTTCAGGGCATGTCCTTTATAGCTGCAGTGTTGATCTTGAACCTCGATACTGCAGATGCCTTTA TTGCTTTTTCTAATCTTTTGAATAAACCCTGTCAAATGGCGTTTTTCCGAGTGGACCATGGCCTTATGTT GACTTACTTTGCTGCATTTGAGGTATTCTTTGAAGAAAACTTGCCGAAATTGTTTGCGCATTTCAAGAAA AACAACTTAACTGCAGATATCTACCTAATCGATTGGATTTTCACCTTGTACAGTAAGTCCCTGCCCTTGG ACTTGGCCTGCCGCATCTGGGACGTATTCTGTCGAGATGGAGAGGAATTTCTCTTCCGCACAGCTCTGGG CATCCTGAAGCTTTTTGAAGATATCCTGACCAGGATGGACTTCATCCACAGTGCCCAGTTTCTGACCAGG CTGCCTGAGGACCTGCCCGCAGATGAAGTGTTTGCAGCTATCTCCACAGTCCAGATGCAAAGCCGGAACA AGAAATGGGCACAGGTGCTGTCTGCACTGCAGAAAGACAGCCGCGAGATGGAGAAAGGAAGCCCATCCCT CAGACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209982 representing BC042515 Red=Cloning site Green=Tags(s) MGILDGRQGDSLQDLQHLSIKAAPRSLSVPEYGPSLKLGALEDRHSLQSVDSGIPTLEIGNPEPVPCSVV HVKRKQSESEIVPERAFQSACPLPSCTPSAPTCSEREQVVRKSSTFPRTGYDSVKLYSPTSKALSRSDNV SVCSVSSLGTELSTTLSVSNEDILDLMVTSNSSAIVTLENDDDPQFTDVTLSSINETSDLHQQDCVAETE EGRKLKLLQPFSHFFTRNLLARKQNARLDRQRDLGWKLFGKVPLRETAQKDSKKTQKEYEDKAGRPSRPP SPKQNVRKNLDFEPLSTTALILEDRPANLPAKPAEEAQKHRQQYEEMVLQAKKRELKEAQRRRKQLEERC KVEESIGNAVLTWNNEILPNWETMWCSKKVRDLWWQGIPPSVRGKVWSLAIGNELNITHELFDICLARAK ERWRSLSTGGSEVENEDAGFSAADREASLELIKLDISRTFPNLCIFQQGGPYHDMLHSILGAYTCYRPDV GYVQGMSFIAAVLILNLDTADAFIAFSNLLNKPCQMAFFRVDHGLMLTYFAAFEVFFEENLPKLFAHFKK NNLTADIYLIDWIFTLYSKSLPLDLACRIWDVFCRDGEEFLFRTALGILKLFEDILTRMDFIHSAQFLTR LPEDLPADEVFAAISTVQMQSRNKKWAQVLSALQKDSREMEKGSPSLRH TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC042515
ORF Size	2039 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC042515, AAH42515
RefSeq Size	4580 bp
RefSeq ORF	2039 bp
Locus ID	100855
Gene Summary	Negative regulator of starvation-induced autophagosome formation.[UniProtKB/Swiss-Prot Function]

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