Tcf25 (BC025071) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Nulp1, mKIAA1049
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209961 representing BC025071 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGCCGGGCCCTCCGGAGACTGAGGGGGGAACAGCGCGGCCAGGAACCCCTAGGGCCCGACGCCT TGAAGTTTGTCCTCCTTGATGACGATGACGCAGAGGAAGAGGGACCAAAACCGGGGCTGGGTGGCCGGCG CCCCGGGGGTGCAGGGAAGGAAGGAGTCCGTGTCAACAACCGGTTTGAGCTGATAAACACGGAGGATCTT GAGGATGACCTTGTGGTAAATGGGGAGAGATCTGACTGTACCCTGCCGGACTCTGTGTCATCAGGGAACA AAGGAAGGGCTAAACATGGAAACGCGGAGACCAAGCAAGATGGAGGGGCCACCAAAGCAGGGTCCTCGGA ACAGTCTAATGCAAGTGGCAAACTCCGAAAGAAGAAAAAGAAGCAGAAAAATAAGAAAAGCTGCACAGGA GAATCCTCGGAGAATGGACTAGAAGACATTGATCGCATCTTAGAGCGGATTGAGGACAGCAGTGGGTTCA GCCACCCCGGCCCCCCTCCACTGAGCTCCAGGAAGCACGTCCTATATGTGGAGCACAGACACCTGAATCC AGACACGGAACTGAAGAGGTATTTTGGTGCTCGAGCGGTCCTGGGCGAGCAAAGGCCTCGACAGAGGCAG CGTGTGTATCCCAAGTGTACATGGCTGACGACCCCTAAGAGCACCTGGCCCCGGTACAGCAAACCAGGTC TATCAATGCGGCTGCTGGAGTCCAAGAAAGGCCTTTCTTTCTTTGCATTTGACCATAATGAAGAATACCA ACAAGCACAGCACAAGTTCCTGGTGGCTGTGGAGTCCATGGAGCCAAATAACATTGTGGTTCTGCTCCAG ACAAGCCCCTATCATGTGGACTCCCTGCTGCAGCTCAGTGATGCCTGCCGCTTTCAAGAGGATCAAGAGA TGGCTCGAGACCTTATAGAGAGAGCCCTGTACAGCATGGAGTGTGCATTCCACCCTTTGTTCAGTCTCAC CAGTGGAACTTGCAGGCTGGATTATCGCAGACCTGAGAATAGGAGCTTCTACCTGACCCTCTACAAGCAG ATGAGTTTCTTGGAGAAACGTGGATGCCCACGTACTGCGCTGGAGTACTGCAAGCTCATCCTGAGCCTTG AGCCAGATGAGGACCCCCTGTGCATGTTGCTGCTTATTGACCACCTGGCCTTACGGGCCCGGAACTATGA ATACCTGATTCGCCTCTTCCAGGAATGGGAGGCTCATCGGAACCTGTCCCAGCTCCCAAATTTTGCCTTC TCAGTTCCACTGGCCTATTTCCTTCTGAGTCAGCAAACAGACTTACCTGAGCATGAGCTCAGCTCTGCCA GGCAACAGGCTTCCCTCCTGATACAGCAGGCACTCACCATGTTCCCTGGAGTTCTTATGCCACTGCTGGA GTATTGCAGTGTGCGACCTGATGCCACGGTCTCAAACCACCGCTTCTTTGGGCCGGATGCTGAGATCAGC CAGCCTCCCGCCTTGGGCCAGCTGGTGAGTCTGTACCTGGGGAGGTCACACTTTCTTTGGAAAGAACCCG CCATTATGAGCTGGCTAGAGGAAAATGTCCATGAGGTTCTACAAGCGGTGGATGCTGGAGACCCTGCTGT GGAGGTCTGTGAGAACAGGCGGAAAGTGTTGTACCAGCGTGCGCCCAGAAACATCCACCGCCACGTGATA CTGTCTGAGATCAAGGAGGCTGTTGCTGCCCTACCCTCGGATGTGACCACACAGTCTGTGATGGGGTTCG ACCCTCTGCCTCCCCTGGACACCATCTACTCTTACGTCAGACCAGAGAGGCTGAGTCCAGTCAGCCACGG AAACACAATCGCCCTCTTCTTCCGGTCCTTGTTGCCAAATTATACCACAGAGGGGGAGAGGCTGGAGGAA GGAGTGGCCGGGGGTCCGAACCGCAATCAAGGCTTGAACAGGCTGATGTTGGCCGTACGTGACATGATGG CCAACTTCCACTTCAACGACCTGGAGGTGCCTCGGGAGGACAATCCTGAGGGCGAGGGGGACTGGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209961 representing BC025071 Red=Cloning site Green=Tags(s) MSRRALRRLRGEQRGQEPLGPDALKFVLLDDDDAEEEGPKPGLGGRRPGGAGKEGVRVNNRFELINTEDL EDDLVVNGERSDCTLPDSVSSGNKGRAKHGNAETKQDGGATKAGSSEQSNASGKLRKKKKKQKNKKSCTG ESSENGLEDIDRILERIEDSSGFSHPGPPPLSSRKHVLYVEHRHLNPDTELKRYFGARAVLGEQRPRQRQ RVYPKCTWLTTPKSTWPRYSKPGLSMRLLESKKGLSFFAFDHNEEYQQAQHKFLVAVESMEPNNIVVLLQ TSPYHVDSLLQLSDACRFQEDQEMARDLIERALYSMECAFHPLFSLTSGTCRLDYRRPENRSFYLTLYKQ MSFLEKRGCPRTALEYCKLILSLEPDEDPLCMLLLIDHLALRARNYEYLIRLFQEWEAHRNLSQLPNFAF SVPLAYFLLSQQTDLPEHELSSARQQASLLIQQALTMFPGVLMPLLEYCSVRPDATVSNHRFFGPDAEIS QPPALGQLVSLYLGRSHFLWKEPAIMSWLEENVHEVLQAVDAGDPAVEVCENRRKVLYQRAPRNIHRHVI LSEIKEAVAALPSDVTTQSVMGFDPLPPLDTIYSYVRPERLSPVSHGNTIALFFRSLLPNYTTEGERLEE GVAGGPNRNQGLNRLMLAVRDMMANFHFNDLEVPREDNPEGEGDWD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC025071
ORF Size	2030 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC025071, AAH25071
RefSeq Size	2167 bp
RefSeq ORF	2030 bp
Locus ID	66855
Gene Summary	May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development (By similarity).[UniProtKB/Swiss-Prot Function]

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