Kcnq1 (BC045142) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AW559127; Kcna9; KVLQT1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209902 representing BC045142 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACGGCCTCGTCCCCGCCCAGTGCTGAAAGGAAGCGCGCGGGTTGGAGCCGCCTGCTAGGCGCCC GGCGGGGCAGCGCGGTGGTCAAGAAGTGTCCCTTCTCACTGGAGCTGGCCGAAGGTGGCCCTGAGGGCAG CACGGTCTATGCGCCCATCGCGCCAACCGGAGCCCCCGGGCTCGCGCCCCCCATGTCGACCCCAGTGTCG CCCGCCCCGGCCCCTGCAGACCTCGGCCCACGTCCGCGGGTGAGCCTTGACCCGCGGGTCTCCATCTACA GTGCGCGCCGCCCGCTGCTGGCGCGCACCCACATCCAGGGCCGAGTCTACAACTTCCTCGAGCGCCCCAC GGGTTGGAAGTGTTTCGTGTACCACTTCACCGTCTTCCTCATTGTTCTGGTCTGCCTCATCTTCAGTGTC CTGTCCACTATTGAGCAGTATGCCGCTCTGGCCACCGGGACCCTCTTCTGGATGGAGATTGTCCTTGTGG TGTTCTTTGGGACAGAATATGTGGTCCGCCTCTGGTCTGCAGGCTGCCGCAGCAAGTACGTGGGCATCTG GGGCCGGCTACGTTTTGCCCGGAAGCCCATTTCCATCATTGACCTCATCGTGGTTGTAGCCTCTATGGTT GTCCTCTGCGTGGGTTCCAAAGGACAAGTGTTCGCCACATCAGCTATCAGGGGTATCCGCTTCCTTCAGA TCCTGCGGATGCTGCATGTCGATCGCCAGGGGGGTACCTGGAGGCTCCTGGGCTCTGTAGTCTTCATTCA CCGCCAGGAGCTGATCACCACCCTGTACATTGGCTTTCTGGGCCTTATCTTCTCCTCCTACTTTGTCTAC TTGGCTGAGAAAGATGCGGTGAACGAGTCCGGCCGCATCGAGTTTGGCAGCTACGCAGATGCTCTGTGGT GGGGGGTGGTCACAGTCACTACCATTGGCTACGGGGATAAGGTACCTCAGACGTGGGTTGGGAAGACCAT CGCCTCCTGTTTCTCTGTCTTCGCCATATCCTTCTTTGCACTCCCAGCGGGGATACTTGGCTCTGGGTTC GCGCTGAAGGTCCAGCAGAAGCAGAGGCAGAAGCACTTCAACCGGCAGATCCCAGCTGCAGCCTCACTCA TCCAGACTGCATGGAGGTGCTATGCCGCTGAGAACCCTGACTCAGCCACTTGGAAGATCTATGTCCGGAA GCCTGCTCGGAGTCACACGCTTCTGTCCCCCAGCCCCAAACCTAAAAAGTCTGTCATGGTAAAGAAGAAG AAGTTCAAGCTGGATAAGGATAATGGGATGAGTCCTGGAGAGAAGATGTTCAATGTTCCTCACATCACTT ATGATCCCCCAGAGGATAGGAGGCCAGACCATTTCTCCATTGATGGCTATGACAGCTCAGTAAGGAAGAG CCCTACACTGCTGGAAGTAAGCACACCCCATTTCTTGAGAACAAACAGCTTTGCAGAGGACCTGGACCTG GAAGGGGAGACACTGCTGACCCCCATCACCCATGTGTCACAGCTGCGGGATCACCATCGGGCCACCATCA AGGTCATCAGGCGCATGCAGTACTTTGTAGCCAAGAAGAAATTCCAGCAAGCACGGAAGCCCTACGACGT GCGAGATGTCATCGAGCAGTACTCCCAGGGCCACCTGAACCTTATGGTGCGCATTAAAGAACTACAGAGA AGGCTGGATCAGTCCATTGGGAAGCCATCTTTGTTCATCCCCATCTCAGAAAAGAGCAAAGACCGTGGCA GTAACACCATCGGTGCCCGTCTGAACAGGGTGGAAGACAAGGTGACACAACTGGACCAGAGACTGGTGAT CATCACAGACATGCTCCACCAGCTGCTGTCCATGCAACAAGGTGGTCCAACCTGCAACAGCAGGTCACAA GTTGTAGCCAGCAATGAAGGTGGCTCCATCAACCCTGAGCTCTTCCTACCCAGCAACAGCCTGCCCACCT ACGAACAACTGACTGTGCCCCAGACAGGCCCTGATGAGGGTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209902 representing BC045142 Red=Cloning site Green=Tags(s) MDTASSPPSAERKRAGWSRLLGARRGSAVVKKCPFSLELAEGGPEGSTVYAPIAPTGAPGLAPPMSTPVS PAPAPADLGPRPRVSLDPRVSIYSARRPLLARTHIQGRVYNFLERPTGWKCFVYHFTVFLIVLVCLIFSV LSTIEQYAALATGTLFWMEIVLVVFFGTEYVVRLWSAGCRSKYVGIWGRLRFARKPISIIDLIVVVASMV VLCVGSKGQVFATSAIRGIRFLQILRMLHVDRQGGTWRLLGSVVFIHRQELITTLYIGFLGLIFSSYFVY LAEKDAVNESGRIEFGSYADALWWGVVTVTTIGYGDKVPQTWVGKTIASCFSVFAISFFALPAGILGSGF ALKVQQKQRQKHFNRQIPAAASLIQTAWRCYAAENPDSATWKIYVRKPARSHTLLSPSPKPKKSVMVKKK KFKLDKDNGMSPGEKMFNVPHITYDPPEDRRPDHFSIDGYDSSVRKSPTLLEVSTPHFLRTNSFAEDLDL EGETLLTPITHVSQLRDHHRATIKVIRRMQYFVAKKKFQQARKPYDVRDVIEQYSQGHLNLMVRIKELQR RLDQSIGKPSLFIPISEKSKDRGSNTIGARLNRVEDKVTQLDQRLVIITDMLHQLLSMQQGGPTCNSRSQ VVASNEGGSINPELFLPSNSLPTYEQLTVPQTGPDEGS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC045142
ORF Size	2006 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC045142, AAH45142
RefSeq Size	3140 bp
RefSeq ORF	2006 bp
Locus ID	16535
Gene Summary	Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon (By similarity) (PubMed:16314573, PubMed:11120752, PubMed:15004216). Associates with KCNE beta subunits that modulates current kinetics (By similarity) (PubMed:17597584, PubMed:15004216). Induces a voltage-dependent by rapidly activating and slowly deactivating potassium-selective outward current (By similarity) (PubMed:8900282). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity). During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed:15004216, PubMed:17597584). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (By similarity). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (By similarity). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (PubMed:16314573). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (PubMed:19491250). When associated with KCNE2, forms an heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (By similarity). When associated with KCNE4, inhibits voltage-gated potassium channel activity (By similarity). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (By similarity). Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (By similarity). Binds with phosphatidylinositol 4,5-bisphosphate (By similarity).[UniProtKB/Swiss-Prot Function]

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