Hal (NM_010401) Mouse Tagged ORF Clone
CAT#: MG209814
- TrueORF®
Hal (tGFP-tagged) - Mouse histidine ammonia lyase (Hal)
ORF Plasmid: DDK
"NM_010401" in other vectors (4)
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CNY 6,270.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | his; hist; histidase; Hs; Hsd |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG209814 representing NM_010401
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTAGGTACACAGTGCACGTGCGTGGGGAATGGCTGGCGGTGCCCTGTCAGGATGGGAAGCTCACTG TTGGCTGGCTGGGCCGTGAGGCTGTGCGACGCTACATGAAGAACAAACCGGACAATGGTGGCTTCACTTC CGTGGATGAAGTGCAATTCCTTGTGCATCGGTGCAAGGGCCTGGGCCTGCTGGACAATGAGGACGAGCTG GAGGTGGCCTTAGAGGACAATGAGTTCGTAGAAGTGGTGATTGAAGGTGATGTGATGTCTCCTGATTTCA TTCCGTCGCAGCCAGAAGGAGTTTTCCTATACAGCAAATACCGGGAGCCTGAAAAATACATTGCCCTAGA TGGGGACAGTCTGAGCACAGAGGATCTGGTCAACCTGGGCAAGGGACGTTACAAGATAAAGCTCACTTCG ATTGCTGAGAAGAAAGTGCAGCAGTCCCGGGAGGTCATCGACAGCATCATCAAAGAAAGAACAGTTGTTT ACGGCATCACCACAGGTTTTGGAAAATTTGCTAGAACTGTAATCCCTGCCAATAAGCTACAGGAGCTTCA AGTCAACTTAGTCCGTTCCCATTCTTCAGGTGTTGGGAAACCACTCAGTCCTGAGAGGTGTCGGATGCTC TTGGCTTTGAGGATCAACGTCTTAGCCAAAGGTTACAGTGGGATTTCCTTGGAGACACTGAAACAAGTTA TTGAAGCATTTAATGCCTCCTGCCTGTCCTATGTTCCAGAGAAAGGAACTGTGGGTGCCAGCGGAGACCT TGCCCCACTCTCTCATCTCGCTCTGGGGCTCATCGGAGAAGGAAAGATGTGGTCCCCGAAGAGTGGCTGG GCAGATGCTAAATATGTTCTAGAAGCCCATGGACTGAAACCAATTGTCTTGAAACCAAAAGAAGGCCTGG CACTCATCAACGGGACACAGATGATCACTTCCCTGGGCTGTGAAGCCCTGGAGCGAGCCAGCGCTATTGC CCGGCAAGCTGATATTGTGGCTGCCTTGACCCTGGAGGTGCTGAAGGGCACCACCAAAGCCTTCGATACC GACATCCATGCTGTCCGACCTCACCGCGGGCAAATTGAAGTTGCTTTCCGATTCCGGTCCCTCCTGGACT CGGATCACCACCCATCAGAAATCGCAGAAAGCCACAGGTTCTGCGATCGTGTTCAGGACGCGTACACCTT ACGCTGCTGTCCACAGGTCCACGGTGTGGTGAACGACACAATAGCCTTTGTGAAGGACATCATCACTACA GAACTGAACAGCGCCACAGACAATCCTATGGTCTTTGCCAGTCGAGGGGAGACAATTTCAGGAGGAAACT TCCATGGTGAATACCCAGCCAAAGCCCTGGACTATTTGGCCATTGGTGTCCATGAACTTGCGGCAATTAG TGAAAGAAGAATCGAAAGGCTGTGTAATCCTTCCCTCAGCGAGCTGCCTGCCTTCCTGGTGGCTGAAGGA GGTCTCAATTCTGGGTTCATGATAGCCCACTGCACCGCAGCAGCCCTGGTGTCTGAGAGCAAGGCTCTGT GCCACCCTTCATCTGTGGACTCACTCTCGACCAGTGCTGCTACAGAGGACCACGTCTCCATGGGAGGATG GGCAGCCAGGAAAGCCCTCAGGGTCGTCGAGCACGTGGAACAAGTGCTAGCCATTGAGCTTCTCGCGGCC TGCCAGGGTATAGAGTTTCTGCGCCCCCTGAAAACAACCACTCCGCTGGAGAAGGTGTACGACCTGGTGC GCTCCGTAGTAAGGCCCTGGATAAAAGATCGCTTCATGGCCCCGGACATTGAGGCAGCACACAGGTTGCT TCTTGACCAAAAGGTTTGGGAGGTTGCCGCACCGTACATCGAGAAATACAGAATGGAGCACATTCCAGAA TCCAGACCGCTTTCTCCAACAGCGTTTTCACTAGAATCTCTCCGAAAGAACTCAGCCACAATCCCAGAGT CTGACGATCTT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209814 representing NM_010401
Red=Cloning site Green=Tags(s) MPRYTVHVRGEWLAVPCQDGKLTVGWLGREAVRRYMKNKPDNGGFTSVDEVQFLVHRCKGLGLLDNEDEL EVALEDNEFVEVVIEGDVMSPDFIPSQPEGVFLYSKYREPEKYIALDGDSLSTEDLVNLGKGRYKIKLTS IAEKKVQQSREVIDSIIKERTVVYGITTGFGKFARTVIPANKLQELQVNLVRSHSSGVGKPLSPERCRML LALRINVLAKGYSGISLETLKQVIEAFNASCLSYVPEKGTVGASGDLAPLSHLALGLIGEGKMWSPKSGW ADAKYVLEAHGLKPIVLKPKEGLALINGTQMITSLGCEALERASAIARQADIVAALTLEVLKGTTKAFDT DIHAVRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKDIITT ELNSATDNPMVFASRGETISGGNFHGEYPAKALDYLAIGVHELAAISERRIERLCNPSLSELPAFLVAEG GLNSGFMIAHCTAAALVSESKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVVEHVEQVLAIELLAA CQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLDQKVWEVAAPYIEKYRMEHIPE SRPLSPTAFSLESLRKNSATIPESDDL SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010401 |
ORF Size | 1971 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010401.3, NP_034531.1 |
RefSeq Size | 2758 bp |
RefSeq ORF | 1974 bp |
Locus ID | 15109 |
UniProt ID | P35492 |
Gene Summary | This gene encodes a member of the histidase protein family. The encoded protein is a cytosolic enzyme which catalyzes the first reaction in histidine catabolism. Defects in this protein cause histidinemia, which is characterized by increased histidine in blood, urine, and cerebrospinal fluid. [provided by RefSeq, Dec 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204658 | Hal (untagged) - Mouse histidine ammonia lyase (Hal), (10ug) |
CNY 4,896.00 |
|
MR209814 | Hal (Myc-DDK-tagged) - Mouse histidine ammonia lyase (Hal) |
CNY 4,896.00 |
|
MR209814L3 | Lenti ORF clone of Hal (Myc-DDK-tagged) - Mouse histidine ammonia lyase (Hal) |
CNY 7,600.00 |
|
MR209814L4 | Lenti ORF clone of Hal (mGFP-tagged) - Mouse histidine ammonia lyase (Hal) |
CNY 7,600.00 |