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Srpk1 (NM_016795) Mouse Tagged ORF Clone
CAT＃: MG209739
- TrueORF®
Srpk1 (tGFP-tagged) - Mouse serine/arginine-rich protein specific kinase 1 (Srpk1)
ORF Plasmid: DDK
"NM_016795" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AU017960 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209739 representing NM_016795
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGAAAGTGCTCGCGCTCCAGGCCCGAAAGAAAAGGACCAAGGCCAAGAAGGACAAAGCCCAAA GGAAGCCTGAAACTCAGCACCGAGGCTCTGCACCCCACTCCGAGAGCGACATCCCAGAGCAGGAGGAGGA GATTCTAGGGTCTGACGATGACGAGCAGGAAGACCCCAATGACTACTGTAAAGGAGGTTATCATCTTGTG AAAATTGGAGATCTATTTAACGGGAGATACCATGTGATTCGAAAATTGGGCTGGGGACACTTTTCCACAG TGTGGTTATCATGGGATATTCAGGGAAAGAAGTTTGTAGCAATGAAAGTAGTTAAAAGTGCTGAGCATTA CACAGAAACAGCACTAGATGAAATCCGGCTGCTGAAATCGGTTCGCAACTCAGACCCGAATGATCCAAAT GGAGAAATGGTTGTTCAACTACTAGATGACTTTAAAATATCAGGCGTTAATGGAACACATATCTGCATGG TGTTTGAGGTTTTGGGGCATCATCTACTCAAGTGGATCATCAAGTCCAACTATCAGGGGCTTCCGCTGCC TTGTGTCAAAAAAATTATTCAGCAAGTGCTACAGGGCCTGGATTACTTACACACCAAGTGCCGGATCATC CACACGGACATCAAACCAGAGAACATCTTGTTGTCTGTGAATGAGCAGTACATTCGAAGGCTGGCTGCAG AGGCCACAGAGTGGCAGCGCTCAGGAGCTCCCCCACCATCGGGGTCTGCAGTCAGCACTGCTCCACAGCC TAAGCCAGCTGACAAAATGTCAAAGAATAAGAAGAAGAAATTGAAGAAGAAGCAGAAGCGCCAGGCAGAA TTACTAGAGAAGCGAATGCAGGAAATTGAGGAAATGGAGAAAGAGTCGGGCCCTGGGCAAAAAAGACCTA ACAAGCAAGAAGAATCAGAGAGTCCTGTTGACAGACCCCTGACAGAGAACCCACCTAATAAAATGACCCA AGAGAAACTTGAAGAGTCAAATTCCATTGGCCAGGACCAGACACTCACGGAGCGTGGTGGAGAGGGTGGT GCGCCAGAGATTAATTGCAATGGAGTGATTGGAGTCGTTAATTACCCTGAGAACAGTAATAATGAGACAC TGAGGCATAAAGAGGATCTGCATAATGCTAATGACTGTGATGTCCACACTTTGAAGCAGGAACCTAGTTT CCTAAACTCTTCAAATGGAGACAGCAGTCCATCTCAAGACACAGACTCTTGCACACCCACGGCCTCTGAG ACCATGGTGTGCCAATCCTCTGCAGAGCAGTCACTCACCCGACAGGACATCACCCAGCTGGAGGAGAGCA TTCGGGCAGATACACCTTCTGGGGATGAGCAGGAGCCCAATGGAGCCCTGGACAGCAAAGGAAAATTCTC TGCTGGAAATTTTCTTATTAATCCCCTTGAGCCAAAAAATGCAGAGAAGCTCCAAGTGAAGATCGCAGAC CTCGGGAACGCCTGCTGGGTGCACAAGCATTTCACTGAAGATATCCAGACACGGCAGTATCGGTCTTTGG AAGTCCTGATAGGATCTGGCTACAACACCCCTGCTGACATCTGGAGCACAGCATGCATGGCCTTTGAACT AGCCACAGGGGACTATTTGTTTGAGCCTCATTCAGGGGAGGATTACACACGAGATGAAGACCACATCGCC CTGATCATAGAACTTCTGGGGAAGGTGCCTCGCAAGCTCATTGTGGCAGGAAAATACTCCAAGGAATTTT TCACCAAAAAAGGTGACCTGAAGCACATCACCAAGTTGAAGCCTTGGGGTCTTCTGGAGGTTCTGGTGGA GAAATATGAGTGGCCTCAGGAGGAGGCTGCCGGCTTCACAGATTTCCTGCTGCCCATGTTGGAGCTTATG CCTGAGAAGAGAGCCACTGCTGCTGAGTGTCTCCGGCATCCTTGGCTAAACTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209739 representing NM_016795
Red=Cloning site Green=Tags(s) MERKVLALQARKKRTKAKKDKAQRKPETQHRGSAPHSESDIPEQEEEILGSDDDEQEDPNDYCKGGYHLV KIGDLFNGRYHVIRKLGWGHFSTVWLSWDIQGKKFVAMKVVKSAEHYTETALDEIRLLKSVRNSDPNDPN GEMVVQLLDDFKISGVNGTHICMVFEVLGHHLLKWIIKSNYQGLPLPCVKKIIQQVLQGLDYLHTKCRII HTDIKPENILLSVNEQYIRRLAAEATEWQRSGAPPPSGSAVSTAPQPKPADKMSKNKKKKLKKKQKRQAE LLEKRMQEIEEMEKESGPGQKRPNKQEESESPVDRPLTENPPNKMTQEKLEESNSIGQDQTLTERGGEGG APEINCNGVIGVVNYPENSNNETLRHKEDLHNANDCDVHTLKQEPSFLNSSNGDSSPSQDTDSCTPTASE TMVCQSSAEQSLTRQDITQLEESIRADTPSGDEQEPNGALDSKGKFSAGNFLINPLEPKNAEKLQVKIAD LGNACWVHKHFTEDIQTRQYRSLEVLIGSGYNTPADIWSTACMAFELATGDYLFEPHSGEDYTRDEDHIA LIIELLGKVPRKLIVAGKYSKEFFTKKGDLKHITKLKPWGLLEVLVEKYEWPQEEAAGFTDFLLPMLELM PEKRATAAECLRHPWLNS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016795 |
| ORF Size | 1944 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016795.4 |
| RefSeq Size | 2484 bp |
| RefSeq ORF | 1947 bp |
| Locus ID | 20815 |
| UniProt ID | O70551 |
| Gene Summary | Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Phosphorylates SFRS2, ZRSR2, LBR and PRM1. Phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Can induce splicing of exon 10 in MAPT/TAU (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220071 | Srpk1 (untagged) - Mouse serine/arginine-rich protein specific kinase 1 (Srpk1), (10ug) |
CNY 8,360.00 |
|
| MR209739 | Srpk1 (Myc-DDK-tagged) - Mouse serine/arginine-rich protein specific kinase 1 (Srpk1) |
CNY 4,832.00 |
|
| MR209739L3 | Lenti ORF clone of Srpk1 (Myc-DDK-tagged) - Mouse serine/arginine-rich protein specific kinase 1 (Srpk1) |
CNY 6,460.00 |
|
| MR209739L4 | Lenti ORF clone of Srpk1 (mGFP-tagged) - Mouse serine/arginine-rich protein specific kinase 1 (Srpk1) |
CNY 6,460.00 |
