United States
Germany
Japan
United Kingdom
China
Gramd1a (BC018554) Mouse Tagged ORF Clone
CAT＃: MG209732
- TrueORF®
Gramd1a (tGFP-tagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082)
ORF Plasmid: DDK
"BC018554" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,090.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1300003M23Rik; AI415002; AW547240; D7Bwg0611e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209732 representing BC018554
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGCTGGTACAGTATGCTGTGTCCCACATACAAACAACGCAACGAGGACTTCCGGAAACTGTTCA GCAAACTTCCTGAAGCAGAGCGCCTCATCGTGGATTACTCCTGCGCCCTGCAGAGGGAGATCCTCCTCCA GGGCCGCCTCTACCTCTCTGAGAACTGGATCTGTTTCTACAGCAACATCTTCCGCTGGGAGACCACAATT TCCATCCAGTTGAAGGAAGTGACCTGTCTGAAGAAGGAGAAGACGGCTAAGCTCATCCCCAATGCCATTC AGATCTGTACTGAGAGTGAGAAGCATTTCTTCACTTCCTTCGGGGCCCGAGACCGCTGCTTCCTCCTCAT CTTCCGTCTCTGGCAGAATGCGCTGCTTGAAAAGACACTGAGTCCTCGAGAGCTTTGGCACCTGGTCCAT CAGTGCTATGGCTCGGAGCTGGGCCTCACCAGTGAAGACGAAGACTATGTCTGCCCCTTGCAACTGAATG GTCTTGGGAGTCCCAAAGAGGTGGGAGACATGATCGCACTCAGCGACATCAGTCCCTCGGGGGCGGCTGA CCACAGCCAGGAGCCAAGCCCTGTGGGTTCAAGGCGTGGCCGGGTCACCCCAAACCTCTCTCGAGCCAGC AGTGATGCAGACCATGGGGCCGAGGAGGACAAGGAAGAACAGACGGACAGTCAGCTGGATGCCTCCTCCA GCCAGACAGTGACCCCAGTAGCAGAACCGCTGAGTTCTGAGCCGACACCACCAGATGGGCCCACTTCCTC CTTGGGTCCCTTGGATCTGCTGTCTAGAGAGGAACTATTGACAGACACGAGTAACTCGTCCTCATCCACT GGGGAGGAAGGTGACCTGGCTGCACTGCTGCCTGACCTCTCCGGCCGGCTCCTCATCAACTCCGTCTTCC ACATGGGTGCTGAGCGTCTGCAGCAGATGCTCTTCTCGGACTCTCCGTTCCTGCAAGGCTTCCTGCAGCA GCGCAAGTTCACAGATGTGACCTTGAGCCCTTGGAGCAGTGACAGCAAGTGCCACCAGCGCAGAGTCCTG ACCTACACCATCCCCATTAGCAACCAGCTAGGCCCCAAGAGCGCCTCTGTGGTGGAGACACAGACGCTGT TCCGGCGCGGCCCACAGGCGGGCGGGTGCGTGGTGGACTCTGAGGTGCTCACCCAGGGCATCCCGTACCA GGACTACTTCTACACTGCCCACCGATACTGTATCCTGGGTCTGGCCCGGAACAAGGCCCGGCTTCGTGTA TCCTCAGAGATCCGCTACCGAAAGCAACCGTGGAGCCTCGTAAAGTCGCTCATTGAGAAGAACTCGTGGA GCGGCATCGAAGATTATTTCCACCACCTGGATCGAGAACTCGCCAAGGCAGAAAAGCTGTCCCTGGAGGA AGGAGGGAAAGATACACGTGGACTACTGTCAGGCCTGCGGAGGAGGAAGCGGCCCCTGAGCTGGAGGGGG CACAGAGATGGGCCTCAGCACCCAGACCCAGACCCCTGCACCCAGACCAGCATGCACACCTCAGGTTCCC TCAGCTCTCGCTTCTCGGAACCATCTGTGGATCAGGGCCCTGGGGCAGGCATCCCCAGCGCCCTGGTTCT CATCAGCATTGTCCTCATCGTTCTCATCGCCCTCAATGCCCTCCTCTTCTACCGTCTCTGGTCTCTGGAG AGAACAGCCCATACCTTTGAGTCCTGGCACAGCCTGGCACTGGCCAAGGGCAAGTTCCCCCAGACAGCCA CGGAGTGGGCGGAGATCTTGGCTCTGCAGAAGCACTTCCACAGCGTTGAGGTGCACAAGTGGAGGCAGAT CCTTCGAGCATCCGTGGAGCTCCTGGATGAGATGAAGTTCTCCTTGGAGAAGCTACATCAAGGAATTACT GTCCCGGATCCTCCCCTGGACACTCAGCCACAGCCTGATGACAGTTTTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209732 representing BC018554
Red=Cloning site Green=Tags(s) MQSWYSMLCPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTI SIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVH QCYGSELGLTSEDEDYVCPLQLNGLGSPKEVGDMIALSDISPSGAADHSQEPSPVGSRRGRVTPNLSRAS SDADHGAEEDKEEQTDSQLDASSSQTVTPVAEPLSSEPTPPDGPTSSLGPLDLLSREELLTDTSNSSSST GEEGDLAALLPDLSGRLLINSVFHMGAERLQQMLFSDSPFLQGFLQQRKFTDVTLSPWSSDSKCHQRRVL TYTIPISNQLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRV SSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLDRELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRG HRDGPQHPDPDPCTQTSMHTSGSLSSRFSEPSVDQGPGAGIPSALVLISIVLIVLIALNALLFYRLWSLE RTAHTFESWHSLALAKGKFPQTATEWAEILALQKHFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGIT VPDPPLDTQPQPDDSFP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC018554 |
| ORF Size | 1941 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC018554, AAH18554 |
| RefSeq Size | 2513 bp |
| RefSeq ORF | 1943 bp |
| Locus ID | 52857 |
| Gene Summary | Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461). May play a role in tumor progression (PubMed:27585821).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200835 | Gramd1a (untagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082), (10ug) |
CNY 4,824.00 |
|
| MR209732 | Gramd1a (Myc-DDK-tagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082) |
CNY 4,816.00 |
|
| MR209732L3 | Lenti ORF clone of Gramd1a (Myc-DDK-tagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082) |
CNY 5,610.00 |
|
| MR209732L4 | Lenti ORF clone of Gramd1a (mGFP-tagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082) |
CNY 5,610.00 |
其它Gramd1a产品
