Wac (BC080851) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1110067P07Rik; A230035H12Rik; AI256735; AI256776; Wwp4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209727 representing BC080851 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAATGTATGCGAGGAAACAGCAGAGACTCAGTGATGGCTGTCACGACCGGAGAGGGGACTCTCAGC CTTTCCAGGCACTTAAGTATTCTTCAAAGAGTCACCCCAGTAGTGGTGATCACAGACATGAAAAGATGCG AGACGCCGCAGATCCTTCACCACCAAACAAAATGTTGCGGAGATCTAATAGTCCTGAAAACAAATACAGT GACAGCACAGGTCACAACAAGGCCAAAAACGTGCACACTCAGAGAGTTAGAGAAAGGGACGGTGGGACCA GTTACTCTCCACAAGAAAACTCACACAACCACAGTGCACTTCATAGTTCAAATTCACATTCTTCTAATCC AAGCAATAATCCAAGCAAAACTTCAGATGCACCTTATGATTCTGCAGATGATTGGTCTGAGCACATTAGC TCATCTGGAAAAAAGTACTACTACAATTGTCGCACAGAAGTTTCACAGTGGGAGAAACCAAAAGAGTGGC TTGAAAGAGAACAGAGACAAAAAGAAGCAAATAAGCTGGCAGTTAATAGTTTCCCAAAAGACAGGGATTA CAGAAGAGAGGTGATGCAAGCAACAGCCACTAGTGGGTTCACCAGTGGAATGGAAGACAAGCATTCCAGC GATGCCAGTAGTTTGCTCCCACAGAATATTTTGTCTCAAACAAGCAGACACAATGACAAAGACTACAGAC TGCCAAGAGCAGAGACTCACAGTAGCTCTACGCCAGTACAGCACCCCATCAAACCAGTGGTTCATCCAAC CGCTACCCCAAGCACTGTTCCTTCTAGTCCATTTACGCTTCAGTCTGATCACCAGCCAAAGAAATCATTT GATGCTAATGGAGCATCTACTTTATCCAAACTGCCTACACCCACAGCTTCTCTCCCTGCACAGAAAACAG AGAGAAAAGAATCTGCTCCAGGAGACAAATCAATATCACATTCTTGCACAACTCCTTCCACATCTTCAGC CTCTGGCCTGAACCCTACATCTGCGCCTCCGACATCTGCTTCAGCAGTACCTGTTTCTCCCGTTCCACAA TCAACAATACCTCCATTACTTCAGGACCCAAATCTTTTTAGACAGTTGCTTCCTGCTTTGCAAGCCACGC TACAGCTTAATAATTCTAATGTGGACATATCCAAAATAAATGAAGTTCTTACAGCAGCTGTAACACAAGC TTCCCTGCAGTCTATCATCCACAAGTTTCTTACTGCTGGACCATCTGCTTTCAACATCACCTCACTGATT TCTCAAGCTGCCCAGCTTTCTACACAAGCCCAGCCATCTAATCAGTCTCCAATGTCTCTGACATCTGATG CTTCATCCCCAAGATCCTATGTGTCTCCAAGAATAAGCACACCTCAAACTAATACAGTCCCTATGAAACC TTTGATTAGTACTCCACCTGTTTCATCCCAGCCAAAGGTTAGTACTCCAGTAGTTAAGCAAGGACCAGTG TCACATTCGGCTACACAGCAGCCTGTAACTGCTGACAAGCAGCAGAGCCATGATTCTGTTTCTCCTCGAA GTCTTCAGCGCTTAAGTAGCCAGAGAAGTCCATCACCTGGTCCAAATCATACTTGTAGTAGTAATGCATC AACTGCAACTGTTGTACCACAGAATGCATCTGCTAGACCTGCGTGCTCATTAACACCTACTCTAGCAGCG CACTTCAATGATAATCTCATAAAGCATGTTCAAGGGTGGCCTGCAGACCACGCAGAGAAACAGGCATCAA GATTGCGTGAAGAAGCCCATAATATGGGAAGTGTACATATGTCAGAAATATGTACTGAGTTAAAAAATTT GAGATCATTAGTTCGAGTATGTGAAATTCAAGCAACTTTGCGAGAGCAGAGGATACTATTTTTGAGACAA CAAATTAAGGAACTCGAAAAGCTGAAAAATCAGAATTCCTTCATGGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209727 representing BC080851 Red=Cloning site Green=Tags(s) MVMYARKQQRLSDGCHDRRGDSQPFQALKYSSKSHPSSGDHRHEKMRDAADPSPPNKMLRRSNSPENKYS DSTGHNKAKNVHTQRVRERDGGTSYSPQENSHNHSALHSSNSHSSNPSNNPSKTSDAPYDSADDWSEHIS SSGKKYYYNCRTEVSQWEKPKEWLEREQRQKEANKLAVNSFPKDRDYRREVMQATATSGFTSGMEDKHSS DASSLLPQNILSQTSRHNDKDYRLPRAETHSSSTPVQHPIKPVVHPTATPSTVPSSPFTLQSDHQPKKSF DANGASTLSKLPTPTASLPAQKTERKESAPGDKSISHSCTTPSTSSASGLNPTSAPPTSASAVPVSPVPQ STIPPLLQDPNLFRQLLPALQATLQLNNSNVDISKINEVLTAAVTQASLQSIIHKFLTAGPSAFNITSLI SQAAQLSTQAQPSNQSPMSLTSDASSPRSYVSPRISTPQTNTVPMKPLISTPPVSSQPKVSTPVVKQGPV SHSATQQPVTADKQQSHDSVSPRSLQRLSSQRSPSPGPNHTCSSNASTATVVPQNASARPACSLTPTLAA HFNDNLIKHVQGWPADHAEKQASRLREEAHNMGSVHMSEICTELKNLRSLVRVCEIQATLREQRILFLRQ QIKELEKLKNQNSFMV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC080851
ORF Size	1940 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC080851, AAH80851
RefSeq Size	3282 bp
RefSeq ORF	1940 bp
Locus ID	225131
Gene Summary	Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. Also acts as a negative regulator of basal autophagy. Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation. May negatively regulate the ubiquitin proteasome pathway.[UniProtKB/Swiss-Prot Function]

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