Usp2 (BC017517) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	B930035K21Rik; Ubp41
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209481 representing BC017517 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGCTCTCCTCCACCCTGAAGCGCTATACAGAATCGTCCCGCTACACAGATGCCCCCTATGCCA AACCGGGCTATGGCACCTACACCCCTTCTTCCTATGGGGCCAACCTGGCCGCCTCCTTCCTGGAGAAGGA AAAACTTGGTTTCAAGCCGGTCTCCCCCACCAGCTTCCTCCCACGGCCCCGCACCTACGGCCCCTCCTCC ATTCTGGACTGTGACAGGGGCCGCCCCCTGCTGAGATCTGACATCATTGGGAGTAGTAAGCGGTCAGAGA GCCAGACCCGTGGCAATGAAAGGCCCTCGGGCAGTGGACTCAACGGAGGCAGTGGATTTTCTTATGGAGT TTCCAGCAACTCCCTCAGCTACCTGCCCATGAATGCCAGAGACCAGGGGGTGACCCTGAGCCAGAAGAAA TCGAACAGCCAATCAGACCTGGCCCGGGATTTCTCCAGCCTCCGGACTTCAGATGGCTACCGGACTTCAG ATGGCTACCGGACCTCAGAGGGCTTTAGGATAGACCCTGGAAACCTGGGTCGCAGCCCCATGCTGGCCCG CACGCGTAAGGAGCTCTGTGCCCTGCAGGGCCTCTACCAAGCAGCCAGCCGTTCTGAGTACCTAACAGAC TATCTGGAGAACTATGGTCGAAAGGGCAGTGCACCACAGGTGCTCACGCAAGCCCCTCCTCCCTCCCGAG TCCCTGAAGTCCTCAGTCCCACCTACCGACCAAGTGGCCGTTACACACTGTGGGAGAAGAGCAAGGGCCA GGCCTCTGGGCCCAGCCGCTCCAGTTCTCCAGGGCGAGACACCATGAATTCAAAGAGTGCCCAGGGTCTG GCTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAACTCAATTCTTCAGTGCCTGAGCAACACCCGAG AGCTGAGAGATTACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGCCACACCAGCAGCGCTCACACGGC CCTCATGGAAGAGTTTGCAAAACTAATCCAGACCATATGGACGTCGTCCCCCAATGATGTGGTGAGCCCA TCTGAGTTCAAGACCCAGATCCAGAGATATGCGCCACGCTTCATGGGCTATAATCAGCAGGATGCTCAGG AATTCCTTCGTTTCCTTCTGGATGGTCTCCACAATGAGGTGAACCGGGTGGCAGCAAGGCCTAAGGCCAG CCCTGAGACCCTTGATCATCTCCCTGATGAAGAAAAGGGGCGACAGATGTGGAGGAAGTATCTGGAAAGG GAAGACAGTCGGATTGGGGATCTCTTCGTTGGGCAGCTGAAGAGCTCCCTCACATGCACCGATTGTGGCT ACTGCTCTACAGTCTTCGATCCCTTCTGGGATCTCTCGTTGCCCATCGCAAAGAGAGGTTACCCTGAGGT GACGTTAATGGATTGTATGAGGCTCTTCACCAAAGAGGACATATTGGATGGTGATGAGAAGCCAACTTGC TGCCGCTGCCGAGCCAGAAAACGATGCATAAAAAAGTTCTCTGTCCAGAGGTTCCCAAAGATCTTGGTGC TCCACCTGAAGCGATTCTCAGAATCCAGGATACGAACCAGCAAGCTCACAACATTTGTGAATTTCCCACT AAGAGACCTGGACTTGAGAGAATTTGCTTCAGAAAACACCAACCATGCTGTTTACAACCTGTATGCTGTG TCCAATCACTCCGGAACCACCATGGGAGGCCACTATACAGCCTACTGCCGAAGTCCGGTTACAGGCGAAT GGCACACTTTCAATGATTCCAGTGTCACACCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTATTT GCTCTTCTATGAACTGGCCAGTCCACCCTCCCGTATG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209481 representing BC017517 Red=Cloning site Green=Tags(s) MSQLSSTLKRYTESSRYTDAPYAKPGYGTYTPSSYGANLAASFLEKEKLGFKPVSPTSFLPRPRTYGPSS ILDCDRGRPLLRSDIIGSSKRSESQTRGNERPSGSGLNGGSGFSYGVSSNSLSYLPMNARDQGVTLSQKK SNSQSDLARDFSSLRTSDGYRTSDGYRTSEGFRIDPGNLGRSPMLARTRKELCALQGLYQAASRSEYLTD YLENYGRKGSAPQVLTQAPPPSRVPEVLSPTYRPSGRYTLWEKSKGQASGPSRSSSPGRDTMNSKSAQGL AGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLGHTSSAHTALMEEFAKLIQTIWTSSPNDVVSP SEFKTQIQRYAPRFMGYNQQDAQEFLRFLLDGLHNEVNRVAARPKASPETLDHLPDEEKGRQMWRKYLER EDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDILDGDEKPTC CRCRARKRCIKKFSVQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAV SNHSGTTMGGHYTAYCRSPVTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC017517
ORF Size	1859 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017517, AAH17517
RefSeq Size	2115 bp
RefSeq ORF	1859 bp
Locus ID	53376
Gene Summary	Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (By similarity). Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities (By similarity). Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (By similarity). Has no deubiquitinase activity against p53/TP53 (By similarity). Prevents MDM2-mediated degradation of MDM4 (By similarity). Plays a role in the G1/S cell-cycle progression in normal and cancer cells (By similarity). Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues (PubMed:23213472, PubMed:25238854, PubMed:26756164). Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (PubMed:23213472). Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (PubMed:25238854).[UniProtKB/Swiss-Prot Function]

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