Usp2 (BC017517) Mouse Tagged ORF Clone

CAT#: MG209481

  • TrueORF®

Usp2 (tGFP-tagged) - Mouse ubiquitin specific peptidase 2 (cDNA clone MGC:27630 IMAGE:4506362)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 5,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms B930035K21Rik; Ubp41
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209481 representing BC017517
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCAGCTCTCCTCCACCCTGAAGCGCTATACAGAATCGTCCCGCTACACAGATGCCCCCTATGCCA
AACCGGGCTATGGCACCTACACCCCTTCTTCCTATGGGGCCAACCTGGCCGCCTCCTTCCTGGAGAAGGA
AAAACTTGGTTTCAAGCCGGTCTCCCCCACCAGCTTCCTCCCACGGCCCCGCACCTACGGCCCCTCCTCC
ATTCTGGACTGTGACAGGGGCCGCCCCCTGCTGAGATCTGACATCATTGGGAGTAGTAAGCGGTCAGAGA
GCCAGACCCGTGGCAATGAAAGGCCCTCGGGCAGTGGACTCAACGGAGGCAGTGGATTTTCTTATGGAGT
TTCCAGCAACTCCCTCAGCTACCTGCCCATGAATGCCAGAGACCAGGGGGTGACCCTGAGCCAGAAGAAA
TCGAACAGCCAATCAGACCTGGCCCGGGATTTCTCCAGCCTCCGGACTTCAGATGGCTACCGGACTTCAG
ATGGCTACCGGACCTCAGAGGGCTTTAGGATAGACCCTGGAAACCTGGGTCGCAGCCCCATGCTGGCCCG
CACGCGTAAGGAGCTCTGTGCCCTGCAGGGCCTCTACCAAGCAGCCAGCCGTTCTGAGTACCTAACAGAC
TATCTGGAGAACTATGGTCGAAAGGGCAGTGCACCACAGGTGCTCACGCAAGCCCCTCCTCCCTCCCGAG
TCCCTGAAGTCCTCAGTCCCACCTACCGACCAAGTGGCCGTTACACACTGTGGGAGAAGAGCAAGGGCCA
GGCCTCTGGGCCCAGCCGCTCCAGTTCTCCAGGGCGAGACACCATGAATTCAAAGAGTGCCCAGGGTCTG
GCTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAACTCAATTCTTCAGTGCCTGAGCAACACCCGAG
AGCTGAGAGATTACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGCCACACCAGCAGCGCTCACACGGC
CCTCATGGAAGAGTTTGCAAAACTAATCCAGACCATATGGACGTCGTCCCCCAATGATGTGGTGAGCCCA
TCTGAGTTCAAGACCCAGATCCAGAGATATGCGCCACGCTTCATGGGCTATAATCAGCAGGATGCTCAGG
AATTCCTTCGTTTCCTTCTGGATGGTCTCCACAATGAGGTGAACCGGGTGGCAGCAAGGCCTAAGGCCAG
CCCTGAGACCCTTGATCATCTCCCTGATGAAGAAAAGGGGCGACAGATGTGGAGGAAGTATCTGGAAAGG
GAAGACAGTCGGATTGGGGATCTCTTCGTTGGGCAGCTGAAGAGCTCCCTCACATGCACCGATTGTGGCT
ACTGCTCTACAGTCTTCGATCCCTTCTGGGATCTCTCGTTGCCCATCGCAAAGAGAGGTTACCCTGAGGT
GACGTTAATGGATTGTATGAGGCTCTTCACCAAAGAGGACATATTGGATGGTGATGAGAAGCCAACTTGC
TGCCGCTGCCGAGCCAGAAAACGATGCATAAAAAAGTTCTCTGTCCAGAGGTTCCCAAAGATCTTGGTGC
TCCACCTGAAGCGATTCTCAGAATCCAGGATACGAACCAGCAAGCTCACAACATTTGTGAATTTCCCACT
AAGAGACCTGGACTTGAGAGAATTTGCTTCAGAAAACACCAACCATGCTGTTTACAACCTGTATGCTGTG
TCCAATCACTCCGGAACCACCATGGGAGGCCACTATACAGCCTACTGCCGAAGTCCGGTTACAGGCGAAT
GGCACACTTTCAATGATTCCAGTGTCACACCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTATTT
GCTCTTCTATGAACTGGCCAGTCCACCCTCCCGTATG


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209481 representing BC017517
Red=Cloning site Green=Tags(s)

MSQLSSTLKRYTESSRYTDAPYAKPGYGTYTPSSYGANLAASFLEKEKLGFKPVSPTSFLPRPRTYGPSS
ILDCDRGRPLLRSDIIGSSKRSESQTRGNERPSGSGLNGGSGFSYGVSSNSLSYLPMNARDQGVTLSQKK
SNSQSDLARDFSSLRTSDGYRTSDGYRTSEGFRIDPGNLGRSPMLARTRKELCALQGLYQAASRSEYLTD
YLENYGRKGSAPQVLTQAPPPSRVPEVLSPTYRPSGRYTLWEKSKGQASGPSRSSSPGRDTMNSKSAQGL
AGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLGHTSSAHTALMEEFAKLIQTIWTSSPNDVVSP
SEFKTQIQRYAPRFMGYNQQDAQEFLRFLLDGLHNEVNRVAARPKASPETLDHLPDEEKGRQMWRKYLER
EDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDILDGDEKPTC
CRCRARKRCIKKFSVQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAV
SNHSGTTMGGHYTAYCRSPVTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC017517
ORF Size 1859 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC017517, AAH17517
RefSeq Size 2115 bp
RefSeq ORF 1859 bp
Locus ID 53376
Gene Summary Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (By similarity). Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities (By similarity). Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (By similarity). Has no deubiquitinase activity against p53/TP53 (By similarity). Prevents MDM2-mediated degradation of MDM4 (By similarity). Plays a role in the G1/S cell-cycle progression in normal and cancer cells (By similarity). Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues (PubMed:23213472, PubMed:25238854, PubMed:26756164). Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (PubMed:23213472). Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (PubMed:25238854).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...