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SPIRE2 (BC026502) Mouse Tagged ORF Clone
CAT＃: MG209467
- TrueORF®
SPIRE2 (tGFP-tagged) - Mouse spire homolog 2 (Drosophila) (cDNA clone MGC:31610 IMAGE:4506974)
ORF Plasmid: DDK
"BC026502" in other vectors (3)
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CN¥ 6,840.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Spir-2, Spir2, MGC31610 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209467 representing BC026502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGTCACTGGGCTTTGCCATTTACCGTGCGCTGGACTGGGGGTTAGATGAGAACGAGGAGAGAG AGCTCAGCCCGCAGCTGGAAAGGCTCATCGACCTCATGGCCAACAGCGACTGCGAAGACAGCAGCTGCGG GGCAGCGGATGAGGGCTACGTGGGTCCAGAAGAGGAGGAGGAGGCTGAGGGCGGCCCCCGAGCTGTGCGC ACCTTTGCCCAGGCTATGCGGCTGTGTGCTTTGCGCCTGACCGACCCCCACGGCGCGCAGGCCCACTACC AGGCAGTGTGCCGAGCTCTCTTTGTGGAGACACTGGAGCTTCGTGCCTTCCTGGCCCGGGTGAGGGAGGC CAAAGAGATGCTGAAGAAACTGGGGGAGGAGGAGCCACGGGAGAAGCCACTGGCCGAGCTCGACCACCTG GGACACACAGACTGGGCCCGGCTGTGGGTCCAGCTCATGCGGGAGCTCCGTCACGGGGTAAAGCTGAAGA AGGTGCAGGAGAAGGAGTTCAACCCACTGCCCACCGAGTTCCAGCTCACCCCCTTCGAGATGCTGATGCA GGATATCCGTGCCAGGAACTATAAGCTGCGCAAGGTCATGGTCGATGGGGACATCCCTCCCAGGGTGAAG AAGGACGCACATGAACTTATCCTGGACTTCATTCGTTCTCGGCCTCCACTGAAGCAGGTCTCAGAGAGAC AACTTCGCCCTGTACCACAGAAGCAAAGAACACTGCACGAGAAGATCCTGGAGGAAATCAAGCAGGAGCG GAGGCTTCGTCCAGTCGGGGCCCAGCACTTGGGAGGCCGTGGGTTTGGTTCCCTGCCCTGCATCCTCAAT GCCTGCTCTGGGGACATCAAGTCCACTTCCTGCATCAACCTGTCTGTCACCGATACTGGGAGCGGGAGTC AGCGCCCACGGCCCCGGGTCCTGCTCAAAGCCCCCACCCTTGCAGAGATGGAAGAAATGAACACGTCTGA GGAAGAAGAGTCCCCATGTGGGGAGGTGGCACTGAAGCGGGACCGCTCTTTTTCGGAACACGACCTGGCC CAGCTTCGGAGCGAGATGGCATCTGGCCTTCAGTCAGCCGCTCAGCCCCCAGGAGGGACGGAGCCACCTC GGGCCAGAGCAGGTAGCATGCACTCCTGGAGGCCCAGCAGCCGAGACCAGGGTTTCTGTCCTGTGAGCGG CCAGTCCCAGCCTCTCCCCAGCTCTGCCTTGCCCAGTAGCTTGAGCTCAGTGGATGGACCTGAGGCGGCT TCACCAGATACCAGACACCTGTGGCTGGAGTTCAGCCACCCTGTGGAGAGCCTGGCTCTGACTGTGGAAG AGGTGGTGGACGTGCGCAGGGTGCTGGTGAAGGCGGAGATGGAGAGGTTTCTGCAGGACAAGGAGCTCTT CAGCAGCCTGAAGAGGGGGAAGATTTGCTGTTGCTGCCGAGCCAAGTTTCCCCTGTTTTCCTGGCCACCC ACCTGTCTCTTCTGCAAGAGAGCTGTGTGCACTTCCTGTAGCGTAAAGATGAAGATGCCTTCCAAGAAGT ATGGACACATCCCTGTCTACACACTTGGCTTTGAGAGTCTTCAGAGGGTGCCAACTACCAAAGCCACGCC AACACTGAGGAGAGATGCCTTCCAATCCCTGCAGGGACCAAAGTGGCGGAGCGTGGAGGAGGAGTTCCCC CACATCTATGCCCACGGCTGTGTCCTAAAGGATGTCTGCAGTGACTGCACCAGCTTTGTGGCTGATGTCG TGTGCTCCAGCCGCAAGAGCGTGGATGTCCTCAATGCCACTCCACGACGAAGCCGTCAGACCCAGTCCCT CTATATCCCCAACACCAGGACTCTCAACTTCCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209467 representing BC026502
Red=Cloning site Green=Tags(s) MVQSLGFAIYRALDWGLDENEERELSPQLERLIDLMANSDCEDSSCGAADEGYVGPEEEEEAEGGPRAVR TFAQAMRLCALRLTDPHGAQAHYQAVCRALFVETLELRAFLARVREAKEMLKKLGEEEPREKPLAELDHL GHTDWARLWVQLMRELRHGVKLKKVQEKEFNPLPTEFQLTPFEMLMQDIRARNYKLRKVMVDGDIPPRVK KDAHELILDFIRSRPPLKQVSERQLRPVPQKQRTLHEKILEEIKQERRLRPVGAQHLGGRGFGSLPCILN ACSGDIKSTSCINLSVTDTGSGSQRPRPRVLLKAPTLAEMEEMNTSEEEESPCGEVALKRDRSFSEHDLA QLRSEMASGLQSAAQPPGGTEPPRARAGSMHSWRPSSRDQGFCPVSGQSQPLPSSALPSSLSSVDGPEAA SPDTRHLWLEFSHPVESLALTVEEVVDVRRVLVKAEMERFLQDKELFSSLKRGKICCCCRAKFPLFSWPP TCLFCKRAVCTSCSVKMKMPSKKYGHIPVYTLGFESLQRVPTTKATPTLRRDAFQSLQGPKWRSVEEEFP HIYAHGCVLKDVCSDCTSFVADVVCSSRKSVDVLNATPRRSRQTQSLYIPNTRTLNFQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC026502 |
| ORF Size | 1854 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026502.1 |
| RefSeq Size | 2175 bp |
| RefSeq ORF | 1856 bp |
| Locus ID | 234857 |
| Gene Summary | Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703, PubMed:21983562). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:21983562). Required for asymmetric spindle positioning and asymmetric cell division during oocyte meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR209467 | SPIRE2 (Myc-DDK-tagged) - Mouse spire homolog 2 (Drosophila) (cDNA clone MGC:31610 IMAGE:4506974) |
CN¥ 6,180.00 |
|
| MR209467L3 | Lenti ORF clone of SPIRE2 (Myc-DDK-tagged) - Mouse spire homolog 2 (Drosophila) (cDNA clone MGC:31610 IMAGE:4506974) |
CN¥ 8,080.00 |
|
| MR209467L4 | Lenti ORF clone of SPIRE2 (mGFP-tagged) - Mouse spire homolog 2 (Drosophila) (cDNA clone MGC:31610 IMAGE:4506974) |
CN¥ 8,080.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
