0610010F05Rik (BC072622) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Kiaa1841; mKIAA1841
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209438 representing BC072622 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCGTGGATATCCAGAAAACAATAATTTTCTGAATAATAATAACCAAATGGTATTGGATATGATTC TTTATCCATTGATTGGAATCCCTCAGACCATCAACTGGGAAACTGTGGCAAGGCTTGTGCCTGGATTAAC GCCCAAAGAGTGTGTGAAAAGGTTTGATGAGCTAAAGAGCTGTGGAAGTTCACCTGTTGACAACCAGTAT AACCCTTTAATGGCTACTGGAGAGGGTCCTGTTGAAACTTTAGCCACTTACATTAAATCTTCACTTCTGG ACACACAAGGAGATTTTCAAGAGACTCCTGTTGATCAAGATACAGTTTCTAAAGCAGGAAGACATAGTAT AGCTACCACAAGGAATTGCTCTTCAGAAAGTGAAAACTGTACTGCTCGTAATGCTGGAGAAGAGACTGGA GAATCTGAAGGGCCAAACATGGTGATCCATGTATGTGATGAAGCAAAAAGCTTGAAAGAAGATTTTATTT GCCCACGAGATCTTTTGATATCAGAAATGAAGTACTTTGCTGAATATTTATCCATGGATGCGCAGCGCTG GGAAGAAGTGGACATTTCAGTTCATTGCGATGTTCACATTTTCAACTGGCTGATAAAATATGTTAAACGG AACACTAAGGAGAGTAAAGATTGTGAGATTCCCGCTTTAGAGCCAGGGAATGTCATTTCAATCCTTATTT CATCAGAGTTCTTGAAGATGGATTCACTAGTTGAACAATGTATTCAATATTGCCATAAAAATATGAATGC CATAGTAGCTGCTCCATGCAACATGAACTGTATCAATGCAAACCTTCTTACACGCATAGCTGATCTGTTC ACACACAATGAAATTGATGACTTAAAAGACAAAAAAGATAAGTTTAGGAGTAAACTGTTTTGTAAGAAGA TTGAAAGACTCTTTGATCCTGAATACTCCAATCCAGATTCTAGAAACAATGCAGCAACACTCTATCGATG CTGTTTGTGTAAGAAACTTTTAACCAGAGAAACAGAAAGAAGAATTCCTTGCATCCCTGGAAAAATCAAT GTGGACCGACATGGAAATATTATCTACATCCACATAAGAGATAAAACCTGGGATGTACATGAATATTTGA ATAGTCTTTTTGAAGAATTAAAATCTTGGAGAGATGTTTATTGGCGTTTGTGGGGCACAGTCAACTGGCT GACTTGTTCAAGGTGTTACCAGGCTTTTCTCTGTATTGAGTTCTCACATTGTCAGTATCACTCAGAAGTG GTAGTTTATTCTAGCACAGTAAATTCACTAAACACTGTGGGCACTGGAATTTATCCCTGCTGTAACCAGA AGGTTCTTCGATTTGATCCTACTCAGCTCACAAAGGGCTGTAAGGTGAGGGACCACATGGTGGTTCTTCA TGATCAAGGAGAAAATGATGATTCTCCTTCTTGCCCACCTGCTAAAATACTGGATGATCTGCACAAGCAC AAAGACGTCATTGCTGTGCCTTTTTTAAAAGATGCAGTTAGTGATCCTGGGGTTGGCTCCTGTGATGAAA AAGGCCTGGAATATGAAATTTTACTGGAACCAAATACACCGTGGGGCTCCAAAACTGGGGAGCTCAATGC TTTCTTGTCACTGAAAAACTGGACTCTGCAGTTGAAACAACAGTCGCTGTTCTCAGAAGAGGAAGAGTAT ACCACGGGGTCTGAGGTCACTGAAGATGAAGTCGGTGATGAAGAAGAAATTGCCAAGAAACAAAGGAAAA AGGAGAAGCCAAAGAAATTTACTAAGCCGCCAAAAAAAACAACTGTCCTCACCCTGTTCTCAAAAGAAAG AGAAGACACTGGAGAAGTCAACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209438 representing BC072622 Red=Cloning site Green=Tags(s) MSRGYPENNNFLNNNNQMVLDMILYPLIGIPQTINWETVARLVPGLTPKECVKRFDELKSCGSSPVDNQY NPLMATGEGPVETLATYIKSSLLDTQGDFQETPVDQDTVSKAGRHSIATTRNCSSESENCTARNAGEETG ESEGPNMVIHVCDEAKSLKEDFICPRDLLISEMKYFAEYLSMDAQRWEEVDISVHCDVHIFNWLIKYVKR NTKESKDCEIPALEPGNVISILISSEFLKMDSLVEQCIQYCHKNMNAIVAAPCNMNCINANLLTRIADLF THNEIDDLKDKKDKFRSKLFCKKIERLFDPEYSNPDSRNNAATLYRCCLCKKLLTRETERRIPCIPGKIN VDRHGNIIYIHIRDKTWDVHEYLNSLFEELKSWRDVYWRLWGTVNWLTCSRCYQAFLCIEFSHCQYHSEV VVYSSTVNSLNTVGTGIYPCCNQKVLRFDPTQLTKGCKVRDHMVVLHDQGENDDSPSCPPAKILDDLHKH KDVIAVPFLKDAVSDPGVGSCDEKGLEYEILLEPNTPWGSKTGELNAFLSLKNWTLQLKQQSLFSEEEEY TTGSEVTEDEVGDEEEIAKKQRKKEKPKKFTKPPKKTTVLTLFSKEREDTGEVNF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC072622
ORF Size	1847 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC072622, AAH72622
RefSeq Size	4139 bp
RefSeq ORF	1847 bp
Locus ID	71675

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