Gucy1b3 (BC050945) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	GC-S-beta-1; GCbeta1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209386 representing BC050945 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGGTTTCGTGAACCATGCCCTGGAGCTGCTGGTGATCCGCAATTATGGTCCCGAGGTGTGGGAAG ACATCAAAAAAGAGGCACAGCTGGATGAAGAAGGCCAGTTTCTTGTCAGAATAATATACGATGATTCCAA AACATATGACCTGGTGGCTGCTGCAAGCAAAGTCCTCAACCTCAATGCTGGCGAAATCCTGCAGATGTTT GGGAAGATGTTTTTCGTCTTCTGCCAGGAGTCTGGCTATGATACCATCTTGCGTGTCCTGGGATCTAATG TCAGAGAGTTTTTGCAGAACCTCGATGCCCTGCATGACCACCTCGCCACCATTTACCCAGGGATGCGCGC GCCTTCCTTCAGGTGCACCGATGCGGAGAAAGGCAAAGGGCTCATCCTGCACTACTACTCGGAAAGAGAG GGGCTTCAGGACATCGTGATCGGGATTATCAAGACTGTTGCTCAACAGATACACGGCACTGAGATAGACA TGAAGGTGATTCAGCAAAGAAATGAAGAATGTGATCACACCCAGTTTTTAATTGAAGAAAAGGAATCAAA AGAAGAGGATTTTTATGAAGATCTGGATAGGTTTGAAGAGAATGGTACCCAGGAATCACGTATCAGCCCT TACACCTTCTGCAAAGCATTTCCTTTTCACATCATATTTGACCGGAACCTAGTGGTCACTCAGTGTGGCA ATGCCATCTACAGAGTGCTCCCCCAGCTCCAGCCTGGGAACTGCAGCCTTCTGTCTGTCTTCTCTCTGGT CCGCCCTCACATCGACATCAGTTTCCATGGGATTCTTTCACACATCAATACAGTCTTTGTACTGAGAAGC AAGGAAGGGTTGCTGGATGTGGAGAAACTTGAGTGTGAGGATGAACTGACTGGAGCAGAGATTAGCTGCT TACGTCTCAAAGGCCAAATGATCTACTTACCAGAAGCAGATAGCATCCTCTTCCTCTGTTCACCAAGTGT GATGAACCTGGACGACCTAACAAGAAGAGGCCTGTATCTGAGTGACATCCCTCTCCACGATGCTACCCGA GACCTGGTTCTTTTGGGAGAACAGTTCCGGGAGGAGTACAAACTGACACAAGAGCTGGAAATCCTCACCG ACAGGCTGCAGCTCACACTGAGAGCCTTGGAGGATGAGAAGAAAAAGACAGACACATTGCTGTATTCTGT CCTTCCTCCATCTGTTGCCAATGAGCTGAGACACAAGCGCCCAGTGCCTGCCAAAAGATACGACAATGTG ACCATCCTCTTCAGCGGCATTGTGGGCTTCAATGCTTTCTGTAGCAAGCATGCATCTGGAGAAGGGGCCA TGAAGATTGTCAATCTCCTCAACGATCTCTACACCCGATTTGACACACTGACTGATTCACGAAAAAACCC ATTTGTTTACAAGGTGGAAACAGTTGGTGACAAGTATATGACAGTGAGTGGCTTGCCAGAACCTTGTATC CACCATGCACGGTCCATTTGCCACCTGGCTTTAGACATGATGGAAATTGCTGGTCAAGTTCAAGTAGATG GTGAATCTGTTCAGATAACAATCGGGATCCATACCGGGGAGGTGGTGACAGGTGTGATTGGACAGCGGAT GCCTCGGTATTGTCTCTTTGGGAATACCGTCAACCTCACAAGCAGGACAGAAACCACAGGAGAAAAGGGA AAGATAAATGTTTCCGAATATACATACAGAAAACTCGGATCCACTGTTCCATTTGGAGCACAGAGGCCCA GTGTCTATGAAGGGCAAGAAGGAACCAATGCAAGTCTGGTTCCTATCCAGGAAAAATACAGGCACGGAGG AAACAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209386 representing BC050945 Red=Cloning site Green=Tags(s) MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMF GKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSERE GLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISP YTFCKAFPFHIIFDRNLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRS KEGLLDVEKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATR DLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNV TILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCI HHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKG KINVSEYTYRKLGSTVPFGAQRPSVYEGQEGTNASLVPIQEKYRHGGNK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC050945
ORF Size	1829 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050945, AAH50945
RefSeq Size	3192 bp
RefSeq ORF	1829 bp
Locus ID	54195
Gene Summary	Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.[UniProtKB/Swiss-Prot Function]

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