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Pomt2 (BC052045) Mouse Tagged ORF Clone
CAT＃: MG209338
- TrueORF®
Pomt2 (tGFP-tagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328)
ORF Plasmid: DDK
"BC052045" in other vectors (4)
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CNY 3,800.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209338 representing BC052045
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCGGCCATAGGCGGTGGCCTAGCGGGCTCCGAGCTGCGTCCCCGTAGGGGCCGCTGTGTGCCGC AGGCTGCCAGAGCCGTGAGCCGAGACGTGGTCCCCCAGGCTGCAGCACGGAAACTTAAACGGCCTGCTTG GAGCTCGCGGCGCTTCCAGGCGGCAGGGTGGTGGGCCACGTTGGCTGTGGTGACGCTGCTGTCCTTTGCC ACCCGCTTTCACCGGCTGGACCAGCCTGCTCACATCTGTTGGGATGAGACTCACTTTGGAAAAATGGGAA GTTACTATATTAACCGCACCTTTTTCTTTGATGTGCATCCACCACTGGGAAAGATGCTGATCGGTCTTGC TGGCTACCTGAGCGGGTATGATGGTACCTTTCTGTTCCAGAAGCCTGGGGACAGATACGAGCACCACAGC TACATGGGAATGAGAGGATTCTGTGCTTTCCTGGGTTCCTGGCTGATCCCCTTTGCCTACCTCACCGTAC TGGATCTGTCCAAGTCCTTCCCAGCAGCACTGCTCACCGCTGCCCTTCTCACCTTTGACACAGGATGCCT CACTCTATCCCAGTACATCCTCCTTGACCCCATCCTGATGTTCTTCATCATGGCGGCCATGCTGAGCATG GTCAAGTACAACTCCTGTGCTAACAGGCCTTTCTCTGCCCCCTGGTGGTTCTGGCTCAGCCTGACTGGCA TTAGTCTCGCTGGTGCTTTAGGGGTCAAATTTGTTGGCCTCTTTATCATCGTGCAAGTGGGGTTGAACAC TATTTCAGACCTCTGGCATCTGTTTGGAGACCTCAGCCTTTCACTGGTGACTGTGGGGAAGCACCTAACT GCTCGCATCCTGTGCCTCATAGTGCTGCCTTTGGTGCTCTATGTGACCATTTTTGCTGTTCATGTCATGG TGCTGAATAAAAGTGGTCCTGGTGATGGCTTCTTCAGTTCTGCCTTTCAAGCCCGGCTTTCAGGGAACAG CCTTCACAATGCTTCTATCCCTGAACACCTGGCGTATGGCTCTGTAATCACTGTGAAGAATCTCCGGATG GCCATTGGCTACCTGCACTCTCATAGGCACCTCTACCCTGAGGGCATTGGTGCTCGCCAGCAGCAGGTTA CCACCTATTTGCATAAGGACTACAACAACTTGTGGATTATCAAGAAGTATAATGCCAACACAGATCCCCT AGACCCTTCATTCCCAGTGGAGTTTGTCAGACATGGAGACATCATACGACTAGAACACAAAGAAACTACT CGGAACCTGCACAGTCACTATCATGAGGCTCCCCTGACCCGGAAGCACTATCAGGTCACTGGCTATGGCA TAAATGGGACAGGGGACTCAAATGACTTCTGGCGGATTGAAGTTGTAAATAGAAAATTTGGGAACCGGAT CAAGGTACTGAGAAGTCGAATTCGCCTCATCCATTTGGTCACAGGTTGTGTCCTGGGATCGTCAGGAAAG ATTCTGCCTAAGTGGGGCTGGGAGCAGTTGGAAGTTACCTGTACTCCGTACCTAAAAGAAACCACTAACT CCATTTGGAACATAGAAGAGCATATCAATCCCAAATTGCCAAACATCAGCCTGGATGTGCTGCAGCCCAG TTTTCCTGAGATCTTGCTGGAGTCTCACATGGTCATGATCCGGGGAAATAATGGCCTCAAACCCAAGGAC AATGAGTTCACATCCAAGCCCTGGCACTGGCCTATCAATTATCAGGGCCTGCGCTTCTCAGGGGCCAATG ACACGGACTTCCGAGTCTATCTGCTCGGCAACCCTGTGAGTATCCCAGCTCTGAGTGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209338 representing BC052045
Red=Cloning site Green=Tags(s) MPPAIGGGLAGSELRPRRGRCVPQAARAVSRDVVPQAAARKLKRPAWSSRRFQAAGWWATLAVVTLLSFA TRFHRLDQPAHICWDETHFGKMGSYYINRTFFFDVHPPLGKMLIGLAGYLSGYDGTFLFQKPGDRYEHHS YMGMRGFCAFLGSWLIPFAYLTVLDLSKSFPAALLTAALLTFDTGCLTLSQYILLDPILMFFIMAAMLSM VKYNSCANRPFSAPWWFWLSLTGISLAGALGVKFVGLFIIVQVGLNTISDLWHLFGDLSLSLVTVGKHLT ARILCLIVLPLVLYVTIFAVHVMVLNKSGPGDGFFSSAFQARLSGNSLHNASIPEHLAYGSVITVKNLRM AIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKYNANTDPLDPSFPVEFVRHGDIIRLEHKETT RNLHSHYHEAPLTRKHYQVTGYGINGTGDSNDFWRIEVVNRKFGNRIKVLRSRIRLIHLVTGCVLGSSGK ILPKWGWEQLEVTCTPYLKETTNSIWNIEEHINPKLPNISLDVLQPSFPEILLESHMVMIRGNNGLKPKD NEFTSKPWHWPINYQGLRFSGANDTDFRVYLLGNPVSIPALSA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052045 |
| ORF Size | 1811 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052045, AAH52045 |
| RefSeq Size | 2800 bp |
| RefSeq ORF | 1811 bp |
| Locus ID | 217734 |
| Gene Summary | This gene encodes an integral membrane protein that belongs to the dolichyl-phosphate-mannose-protein mannosyltransferase family. The encoded enzyme is found in the membrane of the endoplasmic reticulum. This protein is a component of the protein O-mannosyltransferase enzyme complex which is involved in modification of the protein alpha-dystroglycan. Mutations in the human gene are a cause of different forms of muscular dystrophy-dystroglycanopathy (MDDG), type A2 (also known as Walker-Warburg syndrome), type B2 and type C2 (also known as limb-girdle muscular dystrophy). [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217986 | Pomt2 (untagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328), (10ug) |
CNY 6,940.00 |
|
| MR209338 | Pomt2 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328) |
CNY 4,488.00 |
|
| MR209338L3 | Lenti ORF clone of Pomt2 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328) |
CNY 5,320.00 |
|
| MR209338L4 | Lenti ORF clone of Pomt2 (mGFP-tagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328) |
CNY 5,320.00 |
