Osbpl10 (BC027256) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4933433D06Rik; C820004B04Rik; OPR-10; ORP-10
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209222 representing BC027256 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATGAGTTCCAAGACTACTCCAGGCTCCCGCAGCCGAAGCCTCACTTTGCTCCCCCATGGAACAC CCAGTTCTGCGTCTCCCTGTAGCCAGAGACATCTCAGTACGGGCGCCCCCGGTGTTGTCTCAGTCACGCG TCACAAGTCGCCTGCAGCTGCCCGAAGAGCCAAGAGTCAGTATTCCGGCCAGCTTCACGAAGTCAGAGAG ATGATGAACCAGGTGGAAGGCCAGCAGAAGAACCTCGTGCATGCCATTGAGTCCCTGCCAGGCTCGGGCC CCCTCACTGCCTTGGACCAGGACCTGCTTCTCCTGAAGGCTACCTCTGCCGCCACCCTCAGCTGTCTCGG GGAGTGCCTCAGCCTGCTTCAGCAGAGTGTGCGCCAGGCAGCGCCACCCAGCCACAAGCCAGGCGCCTCA GAGACCATCCTGGGATGGCATGGGCCCACGTCACATTCCACAGACCAACTGAAAAATGGGACCCTCGGCT CTCTACCCTCCGCCAGTGCCAACATAACATGGGCGATTCTACCAAACTCTGCAGAAGAGGAACACAACTC ACAGCCGGAGCCAGAGCCAGACTCAGGCCCTGAACTGGTTTTGTCCGAAGAAGAACAGAGCGACAATGAG GACAAGGGAGAGGTGGAGCCGGGCGCCATGGAGGATCAGCGTAGTGTGATCCTCCACCTCATCTCGCAGC TCAAGCTGGGCATGGACCTGACCAAGGTGGTGCTCCCCACGTTCATTCTGGAGAAGCGGTCCTTGCTGGA GATGTACGCAGACTTTATGGCGCACCCTGACCTGCTGCTGGCCATCACTGCCGGGGCGACGCCAGAGGAG AGAGTCATTAGCTTCGTGGAGTATTACCTTACAGCCTTCCACGAGGGCCGCAAAGGCACCCTGGCCAAGA AGCCCTACAACCCCATCATCGGCGAGACCTTTCACTGCTCCTGGGAGGTTCCTAAGGACAGAGTCAAGTC GAAGTGGACTTCCCCCCACCCTCCCATTAGTGCTCACGAGCATCCGATGGCCGATGACCCTTCTAAAAGC TATAAGCTCAGGTTTGTGGCTGAGCAGGTATCCCATCATCCGCCCATCTCCTGCTTCTACTGTGAGTGCA AGGAGAAGAGACTGTGTGTCAACACTCACGTATGGACCAAAAGCAAGTTCATGGGCATGTCCGTGGGGGT CTCTATGATAGGGGAAGGTGTGCTTCGACTTCTGGACCACGGGGAAGAGTACGTCTTCACGCTGCCCAGT GCCTACGCCCGCTCCATCCTCACCGTGCCGTGGGTGGAGCTTGGAGGGAAGGTCAACATCAGCTGTGCCA AGACTGGTTACTCTGCCACCGTGACGTTCCACACGAAGCCCTTCTACGGCGGGAAAGTCCACAGGGTCAC AGCTGAAGTGAAACACAACCCAACCAACACCATCGTCTGTAAAGCACATGGGGAGTGGAATGGCACCTTG GAGTTCACCTACAGCAACGGGGAGACCAAAGTCATCGACACAACCACCCTGCCTGTGTATCCTAAGAAGC TTAGACCCCTGGAGAAGCAGGGGCCCATGGAGTCCAGGAACCTCTGGCAGGAAGTCACCCACTACCTGAG GCTTGGGGACATTGACGCAGCCACAGAGCAGAAACGACGCCTGGAGGAGAGGCAGAGGGTGGAGGAGAGG AAACGGGAGACCCTTCGCACACCGTGGCGACCCAAGTATTTCATCCCGGAGGGCGATGGCTGGGTGTACT TCAACCCCCTCTGGAAGACACAC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209222 representing BC027256 Red=Cloning site Green=Tags(s) MEMSSKTTPGSRSRSLTLLPHGTPSSASPCSQRHLSTGAPGVVSVTRHKSPAAARRAKSQYSGQLHEVRE MMNQVEGQQKNLVHAIESLPGSGPLTALDQDLLLLKATSAATLSCLGECLSLLQQSVRQAAPPSHKPGAS ETILGWHGPTSHSTDQLKNGTLGSLPSASANITWAILPNSAEEEHNSQPEPEPDSGPELVLSEEEQSDNE DKGEVEPGAMEDQRSVILHLISQLKLGMDLTKVVLPTFILEKRSLLEMYADFMAHPDLLLAITAGATPEE RVISFVEYYLTAFHEGRKGTLAKKPYNPIIGETFHCSWEVPKDRVKSKWTSPHPPISAHEHPMADDPSKS YKLRFVAEQVSHHPPISCFYCECKEKRLCVNTHVWTKSKFMGMSVGVSMIGEGVLRLLDHGEEYVFTLPS AYARSILTVPWVELGGKVNISCAKTGYSATVTFHTKPFYGGKVHRVTAEVKHNPTNTIVCKAHGEWNGTL EFTYSNGETKVIDTTTLPVYPKKLRPLEKQGPMESRNLWQEVTHYLRLGDIDAATEQKRRLEERQRVEER KRETLRTPWRPKYFIPEGDGWVYFNPLWKTH SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC027256
ORF Size	1775 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027256, AAH27256
RefSeq Size	1886 bp
RefSeq ORF	1775 bp
Locus ID	74486
Gene Summary	Probable lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane. Its ability to bind phosphatidylserine, suggests that it specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P. Plays a role in negative regulation of lipid biosynthesis. Negatively regulates APOB secretion from hepatocytes. Binds cholesterol and acidic phospholipids. Also binds 25-hydroxycholesterol. Binds phosphatidylserine.[UniProtKB/Swiss-Prot Function]

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