Pigt (BC034175) Mouse Tagged ORF Clone

CAT#: MG209067

  • TrueORF®

Pigt (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752)

ORF Plasmid: DDK tGFP



  "BC034175" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms CGI-06, NDAP, Ndap7
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209067 representing BC034175
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCTCGGTTTGCCGTTGCGGTTACTGGTGCTGTTGCTGGTGGGACGGGGCTGCTGTGGCTGCGCGG
AGGGCCCACGCGACAGCCTGCGAGAAGAGCTCGTTATCACTCCACTGCCTTCCGGCGACGTGGCCGCCAC
ATTCCAGTTCCGCACGCGTTGGGATTCGGATCTGCAGCGGGAAGGAGTGTCCCATTACAGGCTCTTCCCT
AAAGCCCTGGGACAGCTGATCTCCAAGTACTCCCTGCGGGAGCTCCACCTGTCGTTCACGCAAGGCTTTT
GGAGGACGCGATACTGGGGGCCACCCTTCCTGCAGGCTCCGTCAGGTGCAGAGCTCTGGGTCTGGTTCCA
AGACACTGTCACCGATGTGGATAAGTCCTGGAGGGAGCTCAGCAATGTCCTCTCGGGGATCTTCTGTGCA
TCCCTCAACTTCATCGACGCCACCAATACCGTCACTCCCACCGCCTCCTTCAAACCTCTGGGACTGGCCA
ATGACACTGACGACTACTTCCTGCGCTACGCTGTGCTGCCTCGGGAGGTTGTCTGCACTGAGAACCTCAC
GCCATGGAAGAAGCTCTTGCCCTGCAGTTCCAAGGCCGGTCTCTCAGTGCTATTGAAAGCAGACCGCTTG
TTCCACACCAGTTACCACTCCCAGGCAGTGCACATCCGGCCTATCTGCAGAAATGCTCACTGTACCAGCA
TCTCCTGGGAGCTGAGGCAGACCCTTTCGGTTGTCTTTGATGCCTTCATCACAGGACAGGGGAAGAAAGA
CTGGTCCCTCTTCCGCATGTTCTCCCGGACTCTCACAGAGGCCTGTCCATTGGCGTCTCAGAGCCTAGTT
TATGTGGACATCACAGGCTACAGCCAGGATAATGAAACACTGGAGGTGAGCCCTCCCCCAACTTCCACAT
ACCAGGATGTCATTTTGGGCACCAGGAAGACCTATGCCGTCTATGACTTGTTTGACACAGCCATGATCAA
TAACTCTCGAAACCTCAACATCCAGCTCAAATGGAAGAGACCCCCAGATAATGAGGCCCTACCCGTGCCC
TTCCTGCATGCGCAGCGGTACGTGAGTGGCTATGGACTGCAGAAGGGAGAGCTGAGTACTCTGCTGTACA
ACTCCCATCCTTATCGGGCCTTCCCCGTGCTGCTGCTGGATGTCGTGCCCTGGTACCTGCGGCTGTATGT
GCACACGCTCACCATTACCTCCAAGGGCAAGGAGAATAAACCAAGTTACATCCACTACCAACCTGCTCAG
GACAGGCAGCAGCCCCACCTCCTGGAGATGCTCATTCAGCTGCCAGCCAACTCCGTCACCAAGGTCTCCA
TCCAGTTTGAACGAGCCCTGCTCAAGTGGACAGAGTACACACCAGACCCCAACCACGGCTTCTATGTCAG
CCCATCTGTCCTCAGCGCCCTTGTGCCCAGTGTGGTGGCAGCCAAACCAGTGGACTGGGAAGGGAGCCCT
CTCTTCAACACCTTGTTCCCGGTGTCTGATGGCTCCAGCTACTTTGTCCGACTCTACACAGAGCCCTTGC
TAGTGAACCTGCCCACCCCGGACTTCAGCATGCCCTACAATGTGATCTGCCTTACGTGCACGGTGGTGGC
CGTGTGCTATGGCTCCTTCTACAATCTCCTCACCCGAACCTTTCACATCGAAGAGCCCAAGTCGGGCGGC
CTGGCCAAGCGGCTGGCTAACCTCATCCGCCGTGCCCGTGGTGTGCCCCCTCTC


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209067 representing BC034175
Red=Cloning site Green=Tags(s)

MPLGLPLRLLVLLLVGRGCCGCAEGPRDSLREELVITPLPSGDVAATFQFRTRWDSDLQREGVSHYRLFP
KALGQLISKYSLRELHLSFTQGFWRTRYWGPPFLQAPSGAELWVWFQDTVTDVDKSWRELSNVLSGIFCA
SLNFIDATNTVTPTASFKPLGLANDTDDYFLRYAVLPREVVCTENLTPWKKLLPCSSKAGLSVLLKADRL
FHTSYHSQAVHIRPICRNAHCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMFSRTLTEACPLASQSLV
YVDITGYSQDNETLEVSPPPTSTYQDVILGTRKTYAVYDLFDTAMINNSRNLNIQLKWKRPPDNEALPVP
FLHAQRYVSGYGLQKGELSTLLYNSHPYRAFPVLLLDVVPWYLRLYVHTLTITSKGKENKPSYIHYQPAQ
DRQQPHLLEMLIQLPANSVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSVVAAKPVDWEGSP
LFNTLFPVSDGSSYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEEPKSGG
LAKRLANLIRRARGVPPL

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC034175
ORF Size 1734 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC034175.1
RefSeq Size 2322 bp
RefSeq ORF 1736 bp
Locus ID 78928
Gene Summary Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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