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Pigt (BC034175) Mouse Tagged ORF Clone
CAT＃: MR209067
- TrueORF®
Pigt (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752)
ORF Plasmid: tGFP
"BC034175" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-06, NDAP, Ndap7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209067 representing BC034175
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTCGGTTTGCCGTTGCGGTTACTGGTGCTGTTGCTGGTGGGACGGGGCTGCTGTGGCTGCGCGG AGGGCCCACGCGACAGCCTGCGAGAAGAGCTCGTTATCACTCCACTGCCTTCCGGCGACGTGGCCGCCAC ATTCCAGTTCCGCACGCGTTGGGATTCGGATCTGCAGCGGGAAGGAGTGTCCCATTACAGGCTCTTCCCT AAAGCCCTGGGACAGCTGATCTCCAAGTACTCCCTGCGGGAGCTCCACCTGTCGTTCACGCAAGGCTTTT GGAGGACGCGATACTGGGGGCCACCCTTCCTGCAGGCTCCGTCAGGTGCAGAGCTCTGGGTCTGGTTCCA AGACACTGTCACCGATGTGGATAAGTCCTGGAGGGAGCTCAGCAATGTCCTCTCGGGGATCTTCTGTGCA TCCCTCAACTTCATCGACGCCACCAATACCGTCACTCCCACCGCCTCCTTCAAACCTCTGGGACTGGCCA ATGACACTGACGACTACTTCCTGCGCTACGCTGTGCTGCCTCGGGAGGTTGTCTGCACTGAGAACCTCAC GCCATGGAAGAAGCTCTTGCCCTGCAGTTCCAAGGCCGGTCTCTCAGTGCTATTGAAAGCAGACCGCTTG TTCCACACCAGTTACCACTCCCAGGCAGTGCACATCCGGCCTATCTGCAGAAATGCTCACTGTACCAGCA TCTCCTGGGAGCTGAGGCAGACCCTTTCGGTTGTCTTTGATGCCTTCATCACAGGACAGGGGAAGAAAGA CTGGTCCCTCTTCCGCATGTTCTCCCGGACTCTCACAGAGGCCTGTCCATTGGCGTCTCAGAGCCTAGTT TATGTGGACATCACAGGCTACAGCCAGGATAATGAAACACTGGAGGTGAGCCCTCCCCCAACTTCCACAT ACCAGGATGTCATTTTGGGCACCAGGAAGACCTATGCCGTCTATGACTTGTTTGACACAGCCATGATCAA TAACTCTCGAAACCTCAACATCCAGCTCAAATGGAAGAGACCCCCAGATAATGAGGCCCTACCCGTGCCC TTCCTGCATGCGCAGCGGTACGTGAGTGGCTATGGACTGCAGAAGGGAGAGCTGAGTACTCTGCTGTACA ACTCCCATCCTTATCGGGCCTTCCCCGTGCTGCTGCTGGATGTCGTGCCCTGGTACCTGCGGCTGTATGT GCACACGCTCACCATTACCTCCAAGGGCAAGGAGAATAAACCAAGTTACATCCACTACCAACCTGCTCAG GACAGGCAGCAGCCCCACCTCCTGGAGATGCTCATTCAGCTGCCAGCCAACTCCGTCACCAAGGTCTCCA TCCAGTTTGAACGAGCCCTGCTCAAGTGGACAGAGTACACACCAGACCCCAACCACGGCTTCTATGTCAG CCCATCTGTCCTCAGCGCCCTTGTGCCCAGTGTGGTGGCAGCCAAACCAGTGGACTGGGAAGGGAGCCCT CTCTTCAACACCTTGTTCCCGGTGTCTGATGGCTCCAGCTACTTTGTCCGACTCTACACAGAGCCCTTGC TAGTGAACCTGCCCACCCCGGACTTCAGCATGCCCTACAATGTGATCTGCCTTACGTGCACGGTGGTGGC CGTGTGCTATGGCTCCTTCTACAATCTCCTCACCCGAACCTTTCACATCGAAGAGCCCAAGTCGGGCGGC CTGGCCAAGCGGCTGGCTAACCTCATCCGCCGTGCCCGTGGTGTGCCCCCTCTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR209067 representing BC034175
Red=Cloning site Green=Tags(s) MPLGLPLRLLVLLLVGRGCCGCAEGPRDSLREELVITPLPSGDVAATFQFRTRWDSDLQREGVSHYRLFP KALGQLISKYSLRELHLSFTQGFWRTRYWGPPFLQAPSGAELWVWFQDTVTDVDKSWRELSNVLSGIFCA SLNFIDATNTVTPTASFKPLGLANDTDDYFLRYAVLPREVVCTENLTPWKKLLPCSSKAGLSVLLKADRL FHTSYHSQAVHIRPICRNAHCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMFSRTLTEACPLASQSLV YVDITGYSQDNETLEVSPPPTSTYQDVILGTRKTYAVYDLFDTAMINNSRNLNIQLKWKRPPDNEALPVP FLHAQRYVSGYGLQKGELSTLLYNSHPYRAFPVLLLDVVPWYLRLYVHTLTITSKGKENKPSYIHYQPAQ DRQQPHLLEMLIQLPANSVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSVVAAKPVDWEGSP LFNTLFPVSDGSSYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEEPKSGG LAKRLANLIRRARGVPPL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034175 |
| ORF Size | 1734 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC034175.1 |
| RefSeq Size | 2322 bp |
| RefSeq ORF | 1736 bp |
| Locus ID | 78928 |
| MW | 85.1 kDa |
| Gene Summary | Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218498 | Pigt (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752), (10ug) |
CNY 6,940.00 |
|
| MG209067 | Pigt (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752) |
CNY 3,710.00 |
|
| MR209067L3 | Lenti ORF clone of Pigt (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752) |
CNY 5,230.00 |
|
| MR209067L4 | Lenti ORF clone of Pigt (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752) |
CNY 5,230.00 |
