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Ddx27 (BC011321) Mouse Tagged ORF Clone
CAT＃: MG208999
- TrueORF®
Ddx27 (tGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (cDNA clone MGC:18747 IMAGE:4007058)
ORF Plasmid: DDK
"BC011321" in other vectors (4)
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CNY 2,950.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C86129 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208999 representing BC011321
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTCTCCCGGCCCCTCCTGAAGGCCATTACAGCCATGGGCTTCAAGCAGCCCACGCCAATCCAGA AGGCGTGTATCCCTGTGGGTCTGCTGGGGAAGGACATCTGCGCCTGTGCAGCCACTGGGACAGGCAAAAC TGCAGCCTTTGCACTGCCCGTCTTGGAGCGTCTGATCTACAAGCCCCGCCAGGCTGCAGTCACTCGCGTG CTGGTGCTGGTCCCCACGAGAGAGCTGGGCATCCAAGTGCACTCTGTCACCAAGCAGCTGGCCCAGTTCT GCAGCATCACTACCTGCCTGGCTGTGGGTGGCCTGGATGTGAAGTCTCAGGAAGCCGCTCTTCGGGCAGC ACCAGACATCCTCATTGCCACACCTGGCCGGCTCATCGACCACCTCCATAACTGCCCCTCCTTCCACCTG AGCAGCATTGAAGTGCTCATCTTGGATGAGGCTGACAAGATGCTGGATGAGTATTTCGAGGAGCAGATGA AGGAGATTATCCGAATGTGTTCCCACCACCGCCAGACCATGCTCTTCTCGGCCACCATGACAGATGAGGT GAAAGATCTGGCTTCGGTCTCCTTAAAGAATCCTGTGCGGATATTTGTGAACAGCAACACAGATGTCGCT CCCTTCCTGCGGCAAGAGTTCATACGGATCCGGCCTAATAGGGAAGGGGACCGGGAGGCCATTGTGGCAG CTCTGCTGATGAGAACCTTCACTGACCACGTGATGCTCTTCACCCAGACCAAGAAGCAGGCCCATCGCAT GCACATCCTCCTGGGGCTGCTGGGGCTGCAGGTGGGCGAGCTCCACGGCAACTTGTCACAGACCCAGCGC CTGGAGGCCCTCCGGCGCTTTAAGGACGAACAGATTGACATCCTGGTGGCCACAGATGTGGCAGCCCGCG GTCTTGACATTGAAGGAGTGAAAACAGTGATCAACTTCACCATGCCCAACACTGTTAAACACTATGTGCA CCGGGTGGGGCGGACGGCCCGTGCTGGCAGGGCTGGACGCTCTGTCTCCCTAGTGGGAGAGGAGGAGCGG AAGATGCTGAAGGAGATCGTGAAGGCTGCCAAGGCGCCAGTGAAGGCTCGGATCCTTCCCCAGGATGTTA TCCTCAAGTTCCGGGACAAGATTGAGAAATTGGAGAAAGATGTGTATGCAGTGCTGCAGCTGGAGGCGGA GGAAAAAGAGATGCAGCAGTCGGAAGCCCAGATCGACACAGCACAGCGACTCTTAGCCAAAGGGAAAGAG ACAGCAGACCAGGAGCCTGAGAGGAGCTGGTTCCAGACCAAAGAAGAGAGGAAGAAGGAGAAGATTGCTA AGGCTCTACAGGAGTTTGACTTGGCCTTAAGAGGAAAGAAGAAAAGGAAGAAGTTTATGAAGGATGCCAA GAAAAAGGGGGAGATGACGGCAGAGGAGAGGTCCCAGTTTGAAATCCTCAAGGCACAGATGTTTGCAGAG CGGCTGGCCAAGAGGAATCGCAGAACCAAGCGAGCACGGGCAATGCCTGAGGACGAGCCTACAGGCCCTG CTAAAAAGCAGAAGCAGCAGCAGAAGTCTGTGTTTGATGAAGAACTCACCAACACAAGCAAGAAGGCCCT GAAACAGTATCGGGCTGGCCCTTCCTTTGAAGAGAGGAAACAGTCAGGTCTGCCTCGCCAGAGGAGAGGG AACTTCAAATCTAAGTCCAGGTACAAGAGAAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208999 representing BC011321
Red=Cloning site Green=Tags(s) MNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAAVTRV LVLVPTRELGIQVHSVTKQLAQFCSITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHL SSIEVLILDEADKMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVA PFLRQEFIRIRPNREGDREAIVAALLMRTFTDHVMLFTQTKKQAHRMHILLGLLGLQVGELHGNLSQTQR LEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTVKHYVHRVGRTARAGRAGRSVSLVGEEER KMLKEIVKAAKAPVKARILPQDVILKFRDKIEKLEKDVYAVLQLEAEEKEMQQSEAQIDTAQRLLAKGKE TADQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAE RLAKRNRRTKRARAMPEDEPTGPAKKQKQQQKSVFDEELTNTSKKALKQYRAGPSFEERKQSGLPRQRRG NFKSKSRYKRKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC011321 |
| ORF Size | 1718 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC011321, AAH11321 |
| RefSeq Size | 2483 bp |
| RefSeq ORF | 1718 bp |
| Locus ID | 228889 |
| Gene Summary | Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218009 | Ddx27 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (cDNA clone MGC:18747 IMAGE:4007058), (10ug) |
CNY 6,940.00 |
|
| MR208999 | Ddx27 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (cDNA clone MGC:18747 IMAGE:4007058) |
CNY 3,656.00 |
|
| MR208999L3 | Lenti ORF clone of Ddx27 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (cDNA clone MGC:18747 IMAGE:4007058) |
CNY 4,560.00 |
|
| MR208999L4 | Lenti ORF clone of Ddx27 (mGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (cDNA clone MGC:18747 IMAGE:4007058) |
CNY 4,560.00 |
