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Efs (BC031543) Mouse Tagged ORF Clone
CAT＃: MG208875
- TrueORF®
Efs (tGFP-tagged) - Mouse embryonal Fyn-associated substrate (cDNA clone MGC:18407 IMAGE:3669513)
ORF Plasmid: DDK
"BC031543" in other vectors (3)
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CNY 3,520.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208875 representing BC031543
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATTGCCACGTCGGCCCAGCTTGCCCGGGCATTGTATGACAACACTGCTGAGTCCCCCCAGGAAC TGTCCTTCCGCCGAGGGGATGTTCTACGGGTACTGCAGAGGGAGGGTGCTGGTGGACTTGATGGTTGGTG CCTCTGCTCTCTGCATGGCCAGCAGGGTATTGTCCCTGCTAACAGAGTGAAGCTCCTTCCTGCTGGCCCA GCACCCAAGCCTAGCCTCTGCCCAGCATCTCCCACCCAGCCTGGCTCATCATGTCCCACCCCAGAGCGTG GCTGTGAGGAGCAGGAGGTGTATGTAATACCACCACCAGCTCGACCCTGCTCTGCCTCGGGACTTCCAGC TAGATCCTGCTCACCCTCTTCTGACTCCATCTACAAGGTCCCCCGAGTCAATGGGATGCAGCTGACTGCC TCCAGAGACGTGGCAGAGGTCTATGATGTGCCTCCCAACATCCTCCGGGCTCCCTCCAGCTGTCCCTATG ACTCCCCAGCCTCCTTTTCCTGTCCTGTGGCCCCAGTTGTCCCACAGCCCCCCAGAGAGGATGAAGCGCC CTACGATGTGCCTCTGGCCTTAAAGCCACCTGCAGAGCTGGAGCGGGATCTGGAGTGGGAAGGGGGCCGG GAGCCAGGGCCTCCTCTTTACGCTGCCCCTTCCAACCTGAAACGGGCCTCGGCTCTGCTCAACCTGTACG AAGCACCCGAGGAACTCCTGGCAAATGGAGAAAGCAGGGATGCAGATGAAGGCATTTACGATGTGCCTCT GCTGGGGCCAGAGCCACCGTCCCCAGAGCCTCCAGTGGCTTCTTCCTCCACTGACCTGGACACTGTGGCC CAGCTTCCGACCAGAAGCTCCCCACCCCAACACAGGCCCAGGCTACCATCCACTGAGAGCCTGTCCCGCC GCCCTCTGCCTGCCCTCCCTGTCTCTGAGGCTCCTGCTCCTTCTCCAGCTCCTTCTCCTGCCCCAGGCCG AAAAGGCAGTATCCAGGACAGGCCTCTACCCCCTCCCCCACCCTGCCTGCCTGGTTATGGGGGCCTCAAA CCCGAGGGAGATCCAGAGTGCAGGGAGGTAGCCAATGATCCAGCAGGCCCTCACAATGAATATGAAGGCA TCCCAATGGCCGAGGAATATGACTACGTGCATCTGAAGGGTGTGGATACAGCTCAGGGTTCTAGACCCCT GGATAAAGCTTTCCCAGTGGATCCTGAATTGCTGGAGAGGGGACTGGCGGAACGGAAGGAAGCTCTGTCC CCAGAGGAGCCACTGGTCCTGTCCACTGGAGACCTCCAACTTCTGCACTTCTATGCAGGGCAGTGCCAGA GCCATTACTCAGCTCTGCAAGCTGCCGTGGCAGCTCTCGTGGCCAGCACCCAGGCCAACCAGCCACCATG TCTCTTTGTGCCCCACGGCAAAAGAGTGGTGGTGGCTGCTCACCGCCTGGTGTTTGTTGGAGACACCCTA GGCCGGCTGGCAGCCTCTGCAGCTCTCAGAGCACAGGTGGGGGCTGCAGGCACAATGCTGGCCCAAACTC TGCGGGCCACTGTGCTTGCTGTCAAGGGGGCTGCCTTAGGCTACCCATCTGACACTGCAGTCCAAGAGAT GGCGCGATGCGTGGCAGAGCTTGCAGGGCAGGCCCTACGCTTTACCACCCTACTCGCTGGTCTGCTCCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208875 representing BC031543
Red=Cloning site Green=Tags(s) MAIATSAQLARALYDNTAESPQELSFRRGDVLRVLQREGAGGLDGWCLCSLHGQQGIVPANRVKLLPAGP APKPSLCPASPTQPGSSCPTPERGCEEQEVYVIPPPARPCSASGLPARSCSPSSDSIYKVPRVNGMQLTA SRDVAEVYDVPPNILRAPSSCPYDSPASFSCPVAPVVPQPPREDEAPYDVPLALKPPAELERDLEWEGGR EPGPPLYAAPSNLKRASALLNLYEAPEELLANGESRDADEGIYDVPLLGPEPPSPEPPVASSSTDLDTVA QLPTRSSPPQHRPRLPSTESLSRRPLPALPVSEAPAPSPAPSPAPGRKGSIQDRPLPPPPPCLPGYGGLK PEGDPECREVANDPAGPHNEYEGIPMAEEYDYVHLKGVDTAQGSRPLDKAFPVDPELLERGLAERKEALS PEEPLVLSTGDLQLLHFYAGQCQSHYSALQAAVAALVASTQANQPPCLFVPHGKRVVVAAHRLVFVGDTL GRLAASAALRAQVGAAGTMLAQTLRATVLAVKGAALGYPSDTAVQEMARCVAELAGQALRFTTLLAGLLP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031543 |
| ORF Size | 1682 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031543, AAH31543 |
| RefSeq Size | 2373 bp |
| RefSeq ORF | 1682 bp |
| Locus ID | 13644 |
| Gene Summary | Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May serve as an activator of SRC and a downstream effector. Interacts with the SH3 domain of FYN and with CRK, SRC, and YES.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208875 | Efs (Myc-DDK-tagged) - Mouse embryonal Fyn-associated substrate (cDNA clone MGC:18407 IMAGE:3669513) |
CNY 4,168.00 |
|
| MR208875L3 | Lenti ORF clone of Efs (Myc-DDK-tagged) - Mouse embryonal Fyn-associated substrate (cDNA clone MGC:18407 IMAGE:3669513) |
CNY 5,130.00 |
|
| MR208875L4 | Lenti ORF clone of Efs (mGFP-tagged) - Mouse embryonal Fyn-associated substrate (cDNA clone MGC:18407 IMAGE:3669513) |
CNY 5,130.00 |
