Glud1 (BC052724) Mouse Tagged ORF Clone

CAT#: MG208853

  • TrueORF®

Glud1 (tGFP-tagged) - Mouse glutamate dehydrogenase 1 (cDNA clone MGC:64709 IMAGE:6812055)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 6,180.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI118167; Gdh-X; Glud; Gludl
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208853 representing BC052724
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCGCCGTCTGGGCGAAGCGCTGCTGCTGTCCCGCGCCGGGCCCGCTGCCCTGGGCTCTGCGGCTG
CAGACTCAGCCGCGCTGCTGGGCTGGGCCCGCGGACAGCCCTCCGCCGCCCCGCAACCCGGGCTCACGCC
GGTCGCCCGGCGCCACTACAGCGAAGCGGCCGCCGACCGCGAAGACGACCCCAACTTCTTCAAGATGGTG
GAGGGCTTCTTCGACCGCGGCGCCAGCATCGTAGAGGACAAGCTGGTGGAAGACCTGAAGACCCGGGAGA
GCGAGGAGCAGAAGCGGAACCGGGTGCGCGGCATCCTGAGGATCATCAAGCCCTGCAACCATGTGTTGAG
CCTCTCCTTCCCCATCCGGCGCGACGACGGCTCCTGGGAGGTCATCGAAGGCTACCGGGCCCAGCACAGC
CAGCACCGCACGCCCTGCAAGGGAGGTATCCGTTACAGCACTGACGTGAGTGTGGATGAAGTAAAAGCAC
TGGCTTCCTTAATGACATACAAGTGCGCTGTGGTCGATGTACCGTTTGGAGGTGCTAAAGCAGGCGTTAA
GATCAACCCCAAGAACTATACAGATAATGAATTAGAAAAAATTACACGGAGGTTCACTATGGAGCTGGCC
AAGAAGGGTTTTATTGGTCCTGGCATTGATGTGCCTGCCCCAGACATGAGCACGGGTGAGCGGGAGATGT
CCTGGATCGCTGACACCTATGCCAGCACCATAGGGCACTATGATATCAATGCGCATGCCTGTGTTACTGG
GAAACCCATCAGTCAAGGAGGCATCCACGGGCGCATCTCCGCTACTGGCCGGGGTGTCTTCCATGGAATT
GAAAACTTCATCAATGAGGCTTCTTACATGAGCATTTTAGGAATGACACCAGGCTTTGGCGATAAGACAT
TTGTTGTTCAGGGATTTGGTAATGTGGGCCTGCACTCTATGAGATATTTACATCGTTTTGGTGCTAAATG
TGTTGGTGTTGGAGAGTCTGATGGGAGTATATGGAATCCGGATGGGATTGACCCAAAAGAACTGGAAGAC
TTCAAGTTGCAACATGGATCAATTCTGGGCTTCCCCAAAGCCAAGGTCTATGAAGGAAGCATCTTGGAGG
CTGACTGTGACATTCTGATTCCTGCCGCCAGCGAGAAGCAGTTGACCAAATCCAATGCACCCAGAGTCAA
AGCCAAGATCATTGCTGAAGGTGCCAATGGGCCAACCACTCCAGAGGCTGATAAGATTTTCCTGGAAAGA
AACATCATGGTTATTCCCGATCTCTACTTAAATGCTGGAGGAGTAACAGTGTCTTACTTTGAGTGGCTAA
AGAATCTAAATCATGTCAGCTACGGCCGATTGACCTTCAAATATGAAAGGGACTCTAACTACCACTTGCT
CATGTCTGTTCAAGAGAGTTTAGAGAGAAAGTTTGGAAAGCATGGTGGAACTATTCCTGTGGTCCCCACA
GCAGAGTTCCAGGACAGGATATCGGGTGCATCTGAGAAAGACATTGTGCACTCTGGCTTGGCCTACACAA
TGGAGAGATCTGCCAGGCAAATTATGCGCACAGCCATGAAGTATAACCTGGGATTGGACCTGAGAACAGC
TGCCTATGTCAATGCTATCGAGAAAGTCTTCAAGGTGTACAATGAAGCTGGTGTGACCTTCACA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208853 representing BC052724
Red=Cloning site Green=Tags(s)

MYRRLGEALLLSRAGPAALGSAAADSAALLGWARGQPSAAPQPGLTPVARRHYSEAAADREDDPNFFKMV
EGFFDRGASIVEDKLVEDLKTRESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHS
QHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTMELA
KKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGI
ENFINEASYMSILGMTPGFGDKTFVVQGFGNVGLHSMRYLHRFGAKCVGVGESDGSIWNPDGIDPKELED
FKLQHGSILGFPKAKVYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLER
NIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQESLERKFGKHGGTIPVVPT
AEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052724
ORF Size 1676 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052724, AAH52724
RefSeq Size 3045 bp
RefSeq ORF 1676 bp
Locus ID 14661
Gene Summary Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...