Mllt3 (BC021420) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2210011H10Rik; 2610012I03Rik; 3830408D16Rik; Af9; D4Ertd321e
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208832 representing BC021420 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAGCTCGTGTGCCGTGCAGGTGAAGCTGGAGCTGGGGCACCGCGCCCAGGTGAGGAAGAAACCCA CAGTGGAGGGCTTCACCCACGACTGGATGGTGTTCGTGCGCGGCCCGGAGCACAGTAACATTCAGCACTT TGTGGAGAAAGTCGTCTTCCACTTGCACGAAAGCTTCCCTAGGCCAAAAAGAGTGTGCAAAGACCCACCT TACAAGGTAGAAGAGTCCGGGTACGCTGGCTTCATATTGCCAATTGAAGTTTATTTTAAAAACAAGGAAG AACCTAAGAAAGTCCGCTTTGATTATGACTTATTCCTGCATCTTGAAGGCCACCCACCAGTGAATCACCT CCGCTGTGAGAAACTAACTTTCAATAACCCCACGGAGGACTTTAGAAGAAAGTTGCTGAAGGCAGGAGGG GACCCTAACAGGAGCATTCATACCAGCAGTAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCA GCAGTAGCAGTAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTAGTACCAGTTTTTCAAAGCCTCACAAATT AATGAAGGAGCACAAGGAAAAACCTTCTAAGGACTCCAGAGAACATAAAAGTGCCTTCAAAGAACCTTCC AGGGATCACAACAAATCTTCCAAAGACTCCTCCAAGAAACCCAAAGAAAACAAACCACTGAAAGAAGAAA AAATAGTTCCTAAGATGGCCTTCAAGGAACCTAAACCCATGTCAAAAGAGCCAAAAGCAGACAGTAACTT ACTCACCGTCACCAGTGGGCAGCAGGACAAGAAGGCGCCCAGCAAAAGGCCCCCTGCTTCAGATTCTGAA GAACTCTCAGCCAAAAAAAGGAAAAAGAGTAGCTCGGAGGCTTTATTTAAAAGTTTTTCTAGTGCGCCCC CACTGATACTCACTTGTTCTGCTGACAAAAAACAGATAAAAGATAAATCTCATGTCAAGATGGGAAAGGT CAAAATTGAGAGTGAGACTTCAGAGAAGAAGAAGTCAACGTTGCCACCGTTTGATGACATCGTGGATCCC AATGACTCAGACGTGGAGGAGAACATGTCCTCGAAATCTGACTCAGAGCAACCCAGTCCTGCCAGCTCCA GCTCCAGTTCCAGCTCCAGCTTCACACCATCCCAGACCAGGCAACAAGGTCCTTTAAGGTCGATCATGAA AGATCTGCATTCTGATGACAACGAGGAGGAGTCTGATGAGGCAGAGGACAATGACAATGACTCGGAGATG GAAAGACCTGTAAATAGAGGAGGAAGCCGAAGTCGCAGGGTCAGCTTGAGTGATGGCAGTGACAGTGAAA GCAGCTCCGCCTCTTCCCCTCTCCATCATGAACCCCCGCCACCATTATTGAAAACTAACAACAACCAGAT CCTTGAAGTGAAAAGTCCAATAAAACAAAGCAAATCAGATAAGCAAATAAAAAATGGTGAATGTGACAAG GCATATCTAGATGAGTTGGTAGAGCTCCACAGAAGGTTAATGACACTGAGGGAACGACACATTCTGCAGC AGATCGTGAACCTTATAGAGGAAACTGGACACTTTCATATTACGAATACAACATTTGATTTTGATCTTTG CTCGCTGGACAAAACTACAGTCCGTAAATTACAGAGTTACCTGGAAACATCTGGAACATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208832 representing BC021420 Red=Cloning site Green=Tags(s) MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPP YKVEESGYAGFILPIEVYFKNKEEPKKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGG DPNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKDSREHKSAFKEPS RDHNKSSKDSSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKADSNLLTVTSGQQDKKAPSKRPPASDSE ELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKKKSTLPPFDDIVDP NDSDVEENMSSKSDSEQPSPASSSSSSSSSFTPSQTRQQGPLRSIMKDLHSDDNEEESDEAEDNDNDSEM ERPVNRGGSRSRRVSLSDGSDSESSSASSPLHHEPPPPLLKTNNNQILEVKSPIKQSKSDKQIKNGECDK AYLDELVELHRRLMTLRERHILQQIVNLIEETGHFHITNTTFDFDLCSLDKTTVRKLQSYLETSGTS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC021420
ORF Size	1673 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021420, AAH21420
RefSeq Size	3220 bp
RefSeq ORF	1673 bp
Locus ID	70122
Gene Summary	Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.[UniProtKB/Swiss-Prot Function]

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